Date published: 2025-9-6
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ATP1A1/ATP1A2/ATP1A3 Antibody (H-3) - Western Blotting - Image 11797
Na+/K+-ATPase a (H-3): sc-48345. MDCK (A) 和 HeLa (B) 全细胞裂解液中 Na+/K+-ATPase a 表达的 Western 印迹分析.
Na+/K+-ATPase a (H-3): sc-48345. MDCK (A) 和 HeLa (B) 全细胞裂解液中 Na+/K+-ATPase a 表达的 Western 印迹分析.

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3): sc-48345

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说明书
  • ATP1A1/ATP1A2/ATP1A3 抗体 H-3 是小鼠单克隆 IgG2b κ，ATP1A1/ATP1A2/ATP1A3抗体, 在92篇文献中引用，规格为200 µg/ml
  • 针对氨基酸551-850的图谱，在Na+/K+-ATPase α1的内部区域，human起源
  • ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Na+/K+-ATPase α1, 2 and 3; 也和以下物种反应，包括： canine
  • 抗ATP1A1/ATP1A2/ATP1A3抗体（H-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ATP1A1/ATP1A2/ATP1A3 (H-3): sc-48345的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Sodium/Potassium-ATPase Protein 抗体包括 H+/K+ ATPase β, Na+/K+-ATPase α, Na+/K+-ATPase β 1, Na+/K+-ATPase β 3 和 Na+/K+-ATPase α 1

Na+/K+-ATPase α 抗体（H-3）是一种小鼠单克隆 IgG2b kappa 轻链抗体，经过工程改造，可识别小鼠、大鼠、人类和犬的 Na+/K+-ATPase α 亚基（也称为 ATP1A1）。Na+/K+-ATPase α 单克隆抗体（H-3）在蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）中均表现出色。有非结合型和结合型两种形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，Na+/K+-ATPase α (H-3)抗体可满足各种研究需求。Na+/K+-ATPase，通常被称为钠钾泵，是一种重要的质膜蛋白，负责通过主动将三个钠离子运出细胞，将两个钾离子运入细胞，从而维持细胞内环境的稳态。这种活动对于调节细胞体积、维持静息膜电位以及促进多种溶质的二次主动转运至关重要。Na+/K+-ATP酶位于质膜上，在神经冲动传递、肌肉收缩和肾功能等关键生理过程中发挥着重要作用。该蛋白质复合物由α、β和γ亚基的多个同工型组成，其中α亚基含有ATP和转运离子的结合位点，β亚基确保正确的折叠和膜定位，γ亚基调节特定组织中酶的ATP亲和力。了解Na+/K+-ATPase α1的定位和功能对于研究各种细胞机制和疾病状态（如高血压、心脏病和神经系统疾病）至关重要。Na+/K+-ATPase α (H-3) 单克隆抗体使研究人员能够有效地研究这种重要蛋白在不同生物学环境中的表达、调节和作用，从而加深对细胞生理和病理的认识。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) 参考文献:

  1. Na,K-ATPase gamma 亚基的 gamma a 和 gamma b 形式的功能作用和免疫细胞化学定位。  |  Pu, HX., et al. 2001. J Biol Chem. 276: 20370-8. PMID: 11278761
  2. 化学修饰是阐明钠泵结构与功能关系的一种方法。  |  Pedemonte, CH. and Kaplan, JH. 1990. Am J Physiol. 258: C1-23. PMID: 2154108
  3. 钠泵需要其 beta 亚基。  |  McDonough, AA., et al. 1990. FASEB J. 4: 1598-605. PMID: 2156741
  4. Na,K-ATPaseα-亚基和β-亚基在正确的翻译后处理和细胞内转运方面相互依赖。  |  Ackermann, U. and Geering, K. 1990. FEBS Lett. 269: 105-8. PMID: 2167238
  5. 一种与 Na,K-ATP 酶相关的蛋白脂质对 ATP 酶的活性并非必不可少。  |  Hardwicke, PM. and Freytag, JW. 1981. Biochem Biophys Res Commun. 102: 250-7. PMID: 6272791
  6. 异源二聚体 P 型 ATP 酶 beta 亚基的功能意义。  |  Chow, DC. and Forte, JG. 1995. J Exp Biol. 198: 1-17. PMID: 7891030
  7. Na+,K+ -ATP酶。  |  Lingrel, JB. and Kuntzweiler, T. 1994. J Biol Chem. 269: 19659-62. PMID: 8051040
  8. 在酵母细胞中表达功能性钠泵的亚基要求。  |  Scheiner-Bobis, G. and Farley, RA. 1994. Biochim Biophys Acta. 1193: 226-34. PMID: 8054343
  9. Na,K-ATP 酶的α-亚基具有独立于β-亚基的催化活性。  |  Blanco, G., et al. 1994. J Biol Chem. 269: 23420-5. PMID: 8089106
  10. 在杆状病毒感染的昆虫细胞中表达, 定位和组装功能性 Na,K-ATP 酶多肽。  |  DeTomaso, AW., et al. 1993. J Biol Chem. 268: 1470-8. PMID: 8380413
  11. 与 Na,K-ATP 酶相关的小蛋白--γ 多肽的分子克隆和免疫学特征。  |  Mercer, RW., et al. 1993. J Cell Biol. 121: 579-86. PMID: 8387529
  12. Na,K 泵的结构和功能：分子生物学十年。  |  Pressley, TA. 1996. Miner Electrolyte Metab. 22: 264-71. PMID: 8933497

订购信息

产品名称产品编号规格价格数量收藏夹

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3)

sc-48345
200 µg/ml
$316.00

ATP1A1/ATP1A2/ATP1A3 (H-3): m-IgG Fc BP-HRP 套装

sc-528521
200 µg Ab; 10 µg BP
$354.00

ATP1A1/ATP1A2/ATP1A3 (H-3): m-IgGκ BP-HRP 套装

sc-520917
200 µg Ab, 40 µg BP
$354.00

ATP1A1/ATP1A2/ATP1A3 (H-3): m-IgG2b BP-HRP 套装

sc-550049
200 µg Ab; 10 µg BP
$354.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) AC

sc-48345 AC
500 µg/ml, 25% agarose
$416.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) HRP

sc-48345 HRP
200 µg/ml
$316.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) FITC

sc-48345 FITC
200 µg/ml
$330.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) PE

sc-48345 PE
200 µg/ml
$343.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) Alexa Fluor® 488

sc-48345 AF488
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) Alexa Fluor® 546

sc-48345 AF546
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) Alexa Fluor® 594

sc-48345 AF594
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) Alexa Fluor® 647

sc-48345 AF647
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) Alexa Fluor® 680

sc-48345 AF680
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) Alexa Fluor® 790

sc-48345 AF790
200 µg/ml
$357.00

Can Na+/K+-ATPase α Antibody (H-3): sc-48345 be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenni
The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Na+/K+-ATPase α Antibody (H-3): sc-48345 is available in a variety of direct conjugations, such as HRP, PE, FITC, AlexaFluor 488 and AlexaFluor 647.
Answered by: Technical Support 8
Date published: 2017-01-06
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Rated 4 out of 5 by from Good for immunostainingWe use this antibody for immunostaining (1:50 dilution) and can detect.
Date published: 2020-12-02
Rated 5 out of 5 by from good workThis antibody can identify membrane fractions well.
Date published: 2020-10-01
Rated 5 out of 5 by from Great for western blotExcellent for plasma membrane fraction identification.
Date published: 2018-07-24
Rated 3 out of 5 by from Does not work in porcine samples.This did not give a band in western blot of cells from porcine kidney. However, another antibody for the same protein did (C464.8). Note that our own test was in pig cells, and depending on the protein, it is common for antibodies to show some species specificity. We did not test in other species.
Date published: 2018-04-06
Rated 4 out of 5 by from Good Antibody - band at the desired MW.I used this antibody to detect the channel protein in HK2 cells and got band near to the 100kDa marker band.
Date published: 2018-02-22
Rated 5 out of 5 by from Works well for immunostainingTested antibody on coverslip-grown rat myoblast cells. Works well at a 1:100 dilution with overnight incubation at 4˚C.
Date published: 2018-02-13
Rated 5 out of 5 by from WB for mouse brainWestern blot against mouse brain lysate was worked well.
Date published: 2017-11-22
Rated 5 out of 5 by from work well1:1000 used, I am satisfied with the result, this ab has good specificity
Date published: 2017-08-11
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ATP1A1/ATP1A2/ATP1A3 抗体 (H-3) is rated 4.6 out of 5 by 16.
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