Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
ATP1A1/ATP1A2/ATP1A3/ATP1A4 Antibody (M7-PB-E9) - Western Blotting - Image 353138
Na+/K+-ATPase α 抗体 (M7-PB-E9): sc-58628. HeLa (A) 和 Hep G2 (B) 全细胞裂解液以及人脑 (C) 和人肾 (D) 组织提取物中 Na+/K+-ATPase α 表达的 Western 印迹分析.
Na+/K+-ATPase α 抗体 (M7-PB-E9): sc-58628. HeLa (A) 和 Hep G2 (B) 全细胞裂解液以及人脑 (C) 和人肾 (D) 组织提取物中 Na+/K+-ATPase α 表达的 Western 印迹分析.

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9): sc-58628

4.3(10)
寫評論提問

说明书
  • ATP1A1/ATP1A2/ATP1A3/ATP1A4抗体(M7-PB-E9)是小鼠单克隆IgG1 κ, 在33篇文献中引用，规格为200 µg/ml
  • 免疫ovine物种的Na+/K+-ATPase α
  • ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) 推荐用于 WB, IP, IF 和 IHC(P)，检测 all α isoforms of Na+/K+-ATPase of mouse and human origin and Na+/K+-ATPase α3 of rat origin ; 也和以下物种反应，包括： 和 ovine, bovine, porcine and canine
  • 抗ATP1A1/ATP1A2/ATP1A3/ATP1A4抗体（M7-PB-E9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ATP1A1/ATP1A2/ATP1A3/ATP1A4 (M7-PB-E9): sc-58628的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Sodium/Potassium-ATPase Protein 抗体包括 H+/K+ ATPase β, Na+/K+-ATPase α, Na+/K+-ATPase β 1, Na+/K+-ATPase β 3 和 Na+/K+-ATPase α 1

Na+/K+-ATPase α 抗体（M7-PB-E9）是一种小鼠单克隆 IgG1 kappa 轻链抗体，专门用于识别 Na+/K+-ATPase α 亚基。Na+/K+-ATPase α 单克隆抗体（M7-PB-E9）是针对绵羊 Na+/K+-ATPase α 蛋白而制备的，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组化等应用，有效检测小鼠和人类的 α 异构体以及大鼠的 α3 异构体。此外，Na+/K+-ATPase α（M7-PB-E9）抗体还可与其他物种（包括绵羊、牛、猪和犬）发生反应，但不会与章鱼或鸡的α1异构体发生交叉反应，也不会与大鼠的α2或α3异构体发生交叉反应。Na+/K+-ATPase 是一种重要的膜结合酶，由 α、β 和 γ 亚基组成，负责将三个钠离子主动运出细胞，将两个钾离子主动运入细胞，从而维持重要的电化学梯度。这种活动对各种细胞过程至关重要，如调节细胞容积、维持静息膜电位以及促进其他溶质的二次主动转运。α亚基是催化核心，具有与 ATP 和离子结合的位点，而β亚基则确保正确的折叠和膜定位，γ亚基则调节酶对 ATP 的亲和力。Na+/K+-ATPase 与几种调节蛋白相互作用，包括与细胞骨架连接的ankyrin 和参与信号转导途径的 Src 激酶。这些相互作用对于酶调节和细胞信号机制至关重要。Na+/K+-ATPase α（M7-PB-E9）单克隆抗体是研究离子转运动力学、细胞稳态和支配生理过程的复杂蛋白质相互作用的研究人员不可或缺的工具，可在多种物种和实验技术中提供可靠的检测。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9)

sc-58628
200 µg/ml
$316.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 (M7-PB-E9): m-IgG Fc BP-HRP 套装

sc-528627
200 µg Ab; 10 µg BP
$354.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 (M7-PB-E9): m-IgGκ BP-HRP 套装

sc-521056
200 µg Ab, 40 µg BP
$354.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 (M7-PB-E9): m-IgG1 BP-HRP 套装

sc-543037
200 µg Ab; 20 µg BP
$354.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) AC

sc-58628 AC
500 µg/ml, 25% agarose
$416.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) HRP

sc-58628 HRP
200 µg/ml
$316.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) FITC

sc-58628 FITC
200 µg/ml
$330.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) PE

sc-58628 PE
200 µg/ml
$343.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) Alexa Fluor® 488

sc-58628 AF488
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) Alexa Fluor® 546

sc-58628 AF546
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) Alexa Fluor® 594

sc-58628 AF594
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) Alexa Fluor® 647

sc-58628 AF647
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) Alexa Fluor® 680

sc-58628 AF680
200 µg/ml
$357.00

ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) Alexa Fluor® 790

sc-58628 AF790
200 µg/ml
$357.00

Is it possible to use the Ab for IF staining of fixed cells? If yes, what is the suitable Ab concentration to use? Thank you, Gaia

Asked by: Gaia_Liv
Thank you for your question. Na+/K+-ATPase α Antibody (M7-PB-E9): sc-58628 is recommended for detection of all α isoforms of Na+/K+-ATPase of mouse and human origin and Na+/K+-ATPase α3 of rat origin by WB, IP, IF and IHC(P), so yes, it is suitable for what you describe. The appropriate antibody dilution can be found in the product datasheet: starting dilution 1:50, dilution range 1:50-1:500.
Answered by: Tech Support Europe
Date published: 2020-12-08

Does this antibody suitable for fish species?

Asked by: Gayathry
Thank you for your question. This antibody has not been tested for use on fish samples, so we can not recommend nor warranty it for that use. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-07

Can you recommend a positive control to use with Na+/K+-ATPase α (M7-PB-E9): sc-58628 monoclonal antibody for Western blot?

Asked by: jenni
We recommend HeLa (sc-2200) or Hep G2 (sc-2227) whole cell lysates or human brain (sc-364375) or human kidney (sc-363764) tissue extracts to use as positive controls as we have great Western blot data showing specific detection of Na+/K+-ATPase α in these cell lines.
Answered by: Technical Support 13
Date published: 2017-01-20

How can Na+/K+-ATPase α (M7-PB-E9): sc-58628 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Na+/K+-ATPase α (M7-PB-E9): sc-58628 monoclonal antibody is currently available conjugated to phycoerythrin (sc-58628 PE), fluorescein (sc-58628 FITC), Alexa Fluor® 488 (sc-58628 AF488) or Alexa Fluor® 647 (sc-58628 AF647)
Answered by: Technical Support 1
Date published: 2017-01-16
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_58628, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 116ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from NaK antibody for plasma membrane fractionsCritical for establishing fractionation methodology, the NaKATPase antibody played a pivotal role in confirming the presence of NaKATPase in the plasma membrane. Subsequently, its use allowed for the normalization of proteins within this fraction, and the distinct bands obtained facilitated straightforward quantification.
Date published: 2024-01-04
Rated 4 out of 5 by from Buen resultado en Western blotProbé este producto recientemente en Western blot con membranas de eritrocitos de oveja. Obtuve un buen resultado en muestras sin hervir, no funciona bien con muestras hervidas.
Date published: 2018-04-10
Rated 3 out of 5 by from Did not work in porcine samples.This did not give a band in western blot of cells from porcine kidney. However, another antibody for the same protein did (C464.8). Note that our own test was in pig cells, and depending on the protein, it is common for antibodies to show some species specificity. We have not tested in other species.
Date published: 2018-04-06
Rated 5 out of 5 by from The effect is obviousIt was used to detec rat spleen with human kidney tissue, the effect is very good
Date published: 2017-08-21
Rated 2 out of 5 by from Reacts well with human samples.I got a sample of this antibody and it gave me a signal for human sample but not with mouse.
Date published: 2017-07-20
Rated 5 out of 5 by from Outstanding WB and IHCGreat western data; strong signal with low background; also superb IHC data
Date published: 2017-01-25
Rated 5 out of 5 by from Works in many speciesThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-17
Rated 5 out of 5 by from Good antibodyGood antibody, can be used for multiple applications
Date published: 2015-12-10
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_58628, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 20ms
  • REVIEWS, PRODUCT
ATP1A1/ATP1A2/ATP1A3/ATP1A4 抗体 (M7-PB-E9) is rated 4.3 out of 5 by 10.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_10
  • loc_zh_CN, sid_58628, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 105ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。