Date published: 2025-9-9
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N-SMase2 Antibody (G-6) - Western Blotting - Image 312779
N-SMase2 抗体 (G-6): sc-166637. CCRF-CEM (A), Jurkat (B) 和 K-562 (C) 全细胞裂解液中 N-SMase2 表达的 Western 印迹分析.
N-SMase2 抗体 (G-6): sc-166637. CCRF-CEM (A), Jurkat (B) 和 K-562 (C) 全细胞裂解液中 N-SMase2 表达的 Western 印迹分析.

N-SMase2 抗体 (G-6): sc-166637

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说明书
  • N-SMase2 抗体 G-6 是小鼠单克隆 IgG2a κ，N-SMase2抗体, 在33篇文献中引用，规格为200 µg/ml
  • 针对氨基酸461-655的抗原，在N-SMase2的C-terminus处映射，human来源
  • N-SMase2 抗体 (G-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 sphingomyelin phosphodiesterase 3
  • 抗N-SMase2抗体（G-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取N-SMase2 (G-6): sc-166637的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 N-SMase2 抗体 (G-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与N-SMase2 抗体 (G-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

N-SMase 抗体

N-SMase2抗体（G-6）是一种IgG2a κ小鼠单克隆N-SMase2抗体（也称为NSMA2抗体、鞘磷脂磷酸二酯酶3抗体、中性鞘磷脂酶II抗体、中性鞘磷脂酶2抗体、NSMase-2抗体、NSMASE2抗体或鞘磷脂磷酸二酯酶3中性膜（中性鞘磷脂酶II）抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的N-SMase2蛋白。N-SMase2抗体（G-6）既可以是非偶联的抗N-SMase2抗体形式，也可以是多种偶联形式的抗N-SMase2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。N-SMase2（中性鞘磷脂酶2），也称为NSMASE2或SMPD3（鞘磷脂磷酸二酯酶3），是一种普遍表达的655个氨基酸的镁依赖磷酸水解酶蛋白家族成员。N-SMase2定位于高尔基体膜上，其功能是催化鞘磷脂水解形成神经酰胺和磷脂胆碱两种蛋白质，介导细胞生长停滞和凋亡。N-SMase2被不饱和脂肪酸和磷脂酰丝氨酸酶激活，并通过调节神经酰胺的合成参与生长抑制和出生后发育。N-SMase2的表达在细胞周期的G0/G1阶段上调，N-SMase2的最佳活性发生在略偏碱性的pH 7.5。N-SMase2缺乏是导致软骨发育不良的原因，这是一种以骨骼生长受损为特征的遗传性疾病，会导致身材矮小、弓形腿和关节发育不良。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

N-SMase2 抗体 (G-6) 参考文献:

  1. 哺乳动物大脑特异性, Mg2+依赖性中性鞘磷脂酶的克隆和特征。  |  Hofmann, K., et al. 2000. Proc Natl Acad Sci U S A. 97: 5895-900. PMID: 10823942
  2. 哺乳动物中性鞘磷脂酶 2 的生化特性及其在鞘磷脂代谢中的作用。  |  Marchesini, N., et al. 2003. J Biol Chem. 278: 13775-83. PMID: 12566438
  3. 中性鞘磷脂酶 2（smpd3）在控制出生后生长发育中的作用  |  Stoffel, W., et al. 2005. Proc Natl Acad Sci U S A. 102: 4554-9. PMID: 15764706
  4. 编码鞘磷脂磷酸二酯酶 3（Smpd3）的基因缺失会导致小鼠骨生成和牙本质发育不全。  |  Aubin, I., et al. 2005. Nat Genet. 37: 803-5. PMID: 16025116
  5. 肿瘤坏死因子α通过中性鞘磷脂酶 2, 鞘磷脂激酶 1 和 1 磷酸鞘磷脂受体激活内皮一氧化氮合酶：与内皮病理生理学相关的新途径。  |  De Palma, C., et al. 2006. Arterioscler Thromb Vasc Biol. 26: 99-105. PMID: 16269668
  6. 新型肿瘤坏死因子响应型哺乳动物中性鞘磷脂酶-3 是一种 C 尾锚定蛋白。  |  Krut, O., et al. 2006. J Biol Chem. 281: 13784-13793. PMID: 16517606
  7. 氧化应激可调节 nSMase2 的活化和迁移，从而诱导细胞凋亡。  |  Levy, M., et al. 2006. Biochem Biophys Res Commun. 344: 900-5. PMID: 16631623
  8. 中性鞘磷脂酶和 nSMase2：缩小差距。  |  Clarke, CJ. and Hannun, YA. 2006. Biochim Biophys Acta. 1758: 1893-901. PMID: 16938269
  9. 中性鞘磷脂酶（SMPD3）缺乏症会导致一种新型软骨发育不良和侏儒症，Col2A1驱动的smpd3转基因表达可挽救这种疾病。  |  Stoffel, W., et al. 2007. Am J Pathol. 171: 153-61. PMID: 17591962
  10. 音速刺猬素抑制剂环丙胺通过一氧化氮依赖性中性鞘磷脂酶 2/ 甘油酰胺诱导细胞凋亡的脱靶功能  |  Meyers-Needham, M., et al. 2012. Mol Cancer Ther. 11: 1092-102. PMID: 22452947
  11. 鉴定热休克蛋白 60 作为中性鞘磷脂酶 2 的调控因子及其在多巴胺摄取中的作用  |  Ahn, KH., et al. 2013. PLoS One. 8: e67216. PMID: 23840630
  12. 新生儿中风会增强小胶质细胞衍生的细胞外囊泡与小胶质细胞的相互作用。  |  Lecuyer, M., et al. 2021. Neurobiol Dis. 157: 105431. PMID: 34153465

订购信息

产品名称产品编号规格价格数量收藏夹

N-SMase2 抗体 (G-6)

sc-166637
200 µg/ml
$316.00

N-SMase2 (G-6): m-IgG Fc BP-HRP 套装

sc-529014
200 µg Ab; 10 µg BP
$354.00

N-SMase2 (G-6): m-IgGκ BP-HRP 套装

sc-521685
200 µg Ab, 40 µg BP
$354.00

N-SMase2 (G-6): m-IgG2a BP-HRP 套装

sc-547235
200 µg Ab; 10 µg BP
$354.00

N-SMase2 抗体 (G-6) AC

sc-166637 AC
500 µg/ml, 25% agarose
$416.00

N-SMase2 抗体 (G-6) HRP

sc-166637 HRP
200 µg/ml
$316.00

N-SMase2 抗体 (G-6) FITC

sc-166637 FITC
200 µg/ml
$330.00

N-SMase2 抗体 (G-6) PE

sc-166637 PE
200 µg/ml
$343.00

N-SMase2 抗体 (G-6) Alexa Fluor® 488

sc-166637 AF488
200 µg/ml
$357.00

N-SMase2 抗体 (G-6) Alexa Fluor® 546

sc-166637 AF546
200 µg/ml
$357.00

N-SMase2 抗体 (G-6) Alexa Fluor® 594

sc-166637 AF594
200 µg/ml
$357.00

N-SMase2 抗体 (G-6) Alexa Fluor® 647

sc-166637 AF647
200 µg/ml
$357.00

N-SMase2 抗体 (G-6) Alexa Fluor® 680

sc-166637 AF680
200 µg/ml
$357.00

N-SMase2 抗体 (G-6) Alexa Fluor® 790

sc-166637 AF790
200 µg/ml
$357.00

Is it possible to provide the detailed staining protocol of the stained tissue sections which are shown as examples for the nSMase2 antibody (sc-166637)? Thanks in advance

Asked by: acef
Thank you for your question. The assays for creating these images were carried out following our standard protocols, which you can find by following this link: https://www.scbt.com/scbt/resources/protocols
Answered by: Tech Support Europe
Date published: 2020-06-22

To Santa Cruz, I have purchased the antibody for SMPD3 (N-SMase2, G-6, sc-166637), but there are so many bands. It is not clear which band is the one indeed corresponding to that of SMPD3. Could you give a suggestion?

Asked by: ESH11
Thank you for your question. The expected molecular weight for N-SMase2 is around 70-80kDa. Please contact your local distributor or Technical Service directly for assistance regarding your WB results, we are happy to discuss your results with you.
Answered by: Tech Service
Date published: 2019-04-15

What is the recommended secondary to use with this mouse monoclonal antibody?

Asked by: AbPolly
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-16
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Rated 1 out of 5 by from This Ab is not validated by siRNADon't buy this antibody, SCBT please validate your Ab before selling to the public and wasting everybody's time like you wasted my time. Long story short, don't be fooled, while this Ab gives a clean band around 70kDa, this couldn't be verified by siRNA knockdown of nSMase2. The discontinued Rabbit polyclonal Ab was better and was verified perfectly by siRNA knockdown. Don't buy!
Date published: 2020-04-22
Rated 4 out of 5 by from Bastante buena señal para WBLa banda sale un poco más abajo de lo esperado. Cercana a 60kDa, pero se ve clara.
Date published: 2018-12-13
Rated 1 out of 5 by from Don't buy thisAntibody was not good. The rabbit polyclonal which has been stopped was way better than this monoclonal. I did not see any band for my blot even after using the antibody at 1:50.
Date published: 2017-12-17
Rated 5 out of 5 by from good for WB and IFIt works very well for IF staining of PFA fixed cells at a dilution of 1:500 and Western blot at a dilution of 1:8000.Both gave strong singal.
Date published: 2017-08-12
Rated 5 out of 5 by from The experiment was successfulChondrodysplasia is a hereditary disease characterized by impaired bone growth. This is the study is to verify the textbook said, resulting in short stature N-SMase2 defects is the cause of chondrocytosis,
Date published: 2017-01-24
Rated 5 out of 5 by from Clean bands for WBN-SMase2 (G-6) gives a strong signal at 70 kDa in Jurkat whole cell lysate.
Date published: 2016-12-21
Rated 5 out of 5 by from Strong signal strength in WB in CCRFStrong signal strength in WB in CCRF-CEM, Jurkat and K-562 whole cell lysates. -SCBT QC
Date published: 2015-05-04
Rated 5 out of 5 by from Good specificity in WB in IMRGood specificity in WB in IMR-32 whole cell lysate. -SCBT QC
Date published: 2015-03-23
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N-SMase2 抗体 (G-6) is rated 4.1 out of 5 by 10.
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