Date published: 2025-9-6
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myoglobin Antibody (A-6) - Western Blotting - Image 291969
肌红蛋白抗体 (A-6): sc-393020. 大鼠骨骼肌 (A), 大鼠心脏 (B), 人类心脏 (C), 小鼠心脏 (D) 和小鼠骨骼肌 (E) 组织提取物中肌红蛋白表达的 Western 印迹分析.
myoglobin Antibody (A-6) - Western Blotting - Image 291969
肌红蛋白抗体 (A-6): sc-393020. 大鼠骨骼肌 (A), 大鼠心脏 (B), 人类心脏 (C), 小鼠心脏 (D) 和小鼠骨骼肌 (E) 组织提取物中肌红蛋白表达的 Western 印迹分析.
myoglobin Antibody (A-6) - Western Blotting - Image 400035
肌红蛋白抗体 (A-6) HRP: sc-393020 HRP. 小鼠心脏 (A) 和大鼠心脏 (B) 组织提取物中肌红蛋白表达的直接 Western 印迹分析.
myoglobin Antibody (A-6) - Western Blotting - Image 362154
肌红蛋白抗体 (A-6): sc-393020. 人心脏 (A) 和人骨骼肌 (B) 组织提取物中肌红蛋白表达的 Western 印迹分析.
肌红蛋白抗体 (A-6): sc-393020. 大鼠骨骼肌 (A), 大鼠心脏 (B), 人类心脏 (C), 小鼠心脏 (D) 和小鼠骨骼肌 (E) 组织提取物中肌红蛋白表达的 Western 印迹分析.

myoglobin 抗体 (A-6): sc-393020

4.5(2)
寫評論提問

说明书
  • myoglobin 抗体 A-6 是小鼠单克隆 IgG1 κ，myoglobin抗体, 在8篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的myoglobin的C-terminus的氨基酸123-151之间
  • myoglobin 抗体 (A-6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 myoglobin
  • 抗myoglobin抗体（A-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取myoglobin (A-6): sc-393020的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是myoglobin Antibody (A-6) for WB applications. 的首选辅助检测试剂，这些试剂现与myoglobin Antibody (A-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

肌红蛋白抗体（A-6）是一种IgG1κ小鼠单克隆肌红蛋白抗体（也称为MB抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的肌红蛋白蛋白。肌红蛋白抗体（A-6）既可作为非偶联的抗肌红蛋白抗体形式，也可作为多种偶联形式的抗肌红蛋白抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肌红蛋白是一种细胞溶质氧结合蛋白，负责肌细胞内氧的储存和扩散。肌红蛋白的表达在骨骼肌和心肌中最高。肌红蛋白在重度和持续收缩活动期间维持线粒体呼吸是必需的，并且被认为是从红细胞运输氧气到线粒体。肌红蛋白的基因组结构在广泛的物种中似乎是保守的，并且包含一个推定的聚腺苷酸化信号和一个富含嘧啶的区域。人肌红蛋白由一个单一基因指定，已在人类平滑肌中鉴定。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

myoglobin 抗体 (A-6) 参考文献:

  1. CO失活对肌红蛋白功能的影响。  |  Chung, Y., et al. 2006. Am J Physiol Cell Physiol. 290: C1616-24. PMID: 16421206
  2. 工程钌肌红蛋白的氧化还原性质。  |  Li, CZ., et al. 2009. Bioelectrochemistry. 75: 182-8. PMID: 19427819
  3. 用于测定肌红蛋白的定量核磁测定法。  |  Delanghe, JR., et al. 1990. Clin Chem. 36: 1675-8. PMID: 2208710
  4. 人类结合珠蛋白与人类肌红蛋白结合。  |  Sakata, S., et al. 1986. Biochim Biophys Acta. 873: 312-5. PMID: 3756181
  5. 肌红蛋白光产物的低温稳定性。  |  Sassaroli, M., et al. 1986. J Biol Chem. 261: 13704-13. PMID: 3759989
  6. 肌肉毛细血管对外源性肌红蛋白的通透性。  |  Simionescu, N., et al. 1973. J Cell Biol. 57: 424-52. PMID: 4696549
  7. 甲状腺疾病中的血清肌红蛋白。  |  Karlsson, FA., et al. 1980. Acta Endocrinol (Copenh). 94: 184-7. PMID: 6893384
  8. 肌红蛋白诱导的淋巴结细胞对肌红蛋白和肌红蛋白合成抗原肽的体外反应。  |  Stavitsky, AB., et al. 1978. Adv Exp Med Biol. 98: 199-219. PMID: 82384
  9. 氰化氢与肌红蛋白结合的机理。  |  Dou, Y., et al. 1996. Biochemistry. 35: 7107-13. PMID: 8679537
  10. 心肌细胞中肌红蛋白浓度的校准组织化学。  |  Lee-de Groot, MB., et al. 1998. J Histochem Cytochem. 46: 1077-84. PMID: 9705974

订购信息

产品名称产品编号规格价格数量收藏夹

myoglobin 抗体 (A-6)

sc-393020
200 µg/ml
$316.00

myoglobin (A-6): m-IgG Fc BP-HRP 套装

sc-527663
200 µg Ab; 10 µg BP
$354.00

myoglobin (A-6): m-IgG1 BP-HRP 套装

sc-533036
200 µg Ab; 20 µg BP
$354.00

myoglobin 抗体 (A-6) AC

sc-393020 AC
500 µg/ml, 25% agarose
$416.00

myoglobin 抗体 (A-6) HRP

sc-393020 HRP
200 µg/ml
$316.00

myoglobin 抗体 (A-6) FITC

sc-393020 FITC
200 µg/ml
$330.00

myoglobin 抗体 (A-6) PE

sc-393020 PE
200 µg/ml
$343.00

myoglobin 抗体 (A-6) Alexa Fluor® 488

sc-393020 AF488
200 µg/ml
$357.00

myoglobin 抗体 (A-6) Alexa Fluor® 546

sc-393020 AF546
200 µg/ml
$357.00

myoglobin 抗体 (A-6) Alexa Fluor® 594

sc-393020 AF594
200 µg/ml
$357.00

myoglobin 抗体 (A-6) Alexa Fluor® 647

sc-393020 AF647
200 µg/ml
$357.00

myoglobin 抗体 (A-6) Alexa Fluor® 680

sc-393020 AF680
200 µg/ml
$357.00

myoglobin 抗体 (A-6) Alexa Fluor® 790

sc-393020 AF790
200 µg/ml
$357.00

myoglobin (A-6) 中和勝肽

sc-393020 P
100 µg/0.5 ml
$68.00

Thank you for the correction. But which amino acids belong exactly to the epitope of blocking peptide sc-393020 P? You wrote that it is within aa123-151 of P04247. This is a long sequence for a epitope and not exact.

Asked by: Anonymous
The exact peptide sequence used to develop this antibody, and for the blocking peptide (sc-393020 P) is considered proprietary information. We are able to provide a very short range of amino acids 123-151. In addition, the exact epitope recognized by this mouse monoclonal antibody has not been determined.
Answered by: Technical Support
Date published: 2020-03-19

You sent me the following message: Thank you for your question. The blocking peptide maps within amino acids 123-151 of mouse myoglobin, accession#P02144. P02144 is the accession of human myoglobin not of mouse myoglobin. What is correct now?

Asked by: Anonymous
Thank you for your message, and thank you for catching the error in my answer. It is of course the mouse accession number that was supposed to be in the answer. The epitope maps within amino acids 123-151 of accession# P04247.
Answered by: Tech Service
Date published: 2020-03-18

Welche Aminosäure-Sequenz hat das Blocking peptide sc-393020 P?

Asked by: Anonymous
Thank you for your question. The blocking peptide maps within amino acids 123-151 of mouse myoglobin, accession#P02144.
Answered by: Tech Service
Date published: 2020-03-17

What application is the blocking peptide sc-393020 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2020-03-17
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Rated 4 out of 5 by from Works like a charm!Easy to optimize. There is quite a bit of non-specific binding but using 2 types of blocking buffers took care of that; one for blocking overnight and one to dilute the primary antibody in.
Date published: 2018-07-24
Rated 5 out of 5 by from Good Western blot data of myoglobin expressionGood Western blot data of myoglobin expression in rat skeletal muscle, rat heart, human heart, mouse heart and mouse skeletal muscle tissue extracts. -SCBT QC
Date published: 2014-04-28
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myoglobin 抗体 (A-6) is rated 4.5 out of 5 by 2.
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