Date published: 2025-9-7

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myogenin 抗体 (5FD): sc-52903

4.8(14)
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说明书
  • myogenin 抗体 5FD 是小鼠单克隆 IgG1 κ,myogenin抗体, 在82篇文献中引用,规格为200 µg/ml
  • 免疫rat物种的myogenin的氨基酸144-158
  • 推荐用于 mouse, rat 和 human 来源的myogenin WB, IP, IF 和 IHC(P)检测
  • 还可偶联HRP 用于WB, IHC(P) 和ELISA;偶联phycoerythrin 以及 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488Alexa Fluor® 647,用于IF, IHC(P) 和 FCM
  • 关于如何获取myogenin (5FD): sc-52903免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 myogenin 抗体 (5FD) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与myogenin 抗体 (5FD) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

肌细胞生成素抗体(5FD)是一种IgG1 κ小鼠单克隆肌细胞生成素抗体(也被称为MyoD1抗体),可以通过蛋白质印迹法、免疫沉淀法、免疫荧光法和免疫组织化学法(P)检测小鼠、大鼠和人类的肌细胞生成素蛋白。肌细胞生成素抗体(5FD)既有非偶联形式的抗肌细胞生成素抗体,也有多种偶联形式的抗肌细胞生成素抗体,包括辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。肌源性细胞的分化受多种正负作用因子的调节。一个已知在肌肉细胞发育调控中发挥重要作用的特征明确的螺旋-环-螺旋(HLH)蛋白家族包括Myo D、肌细胞生成素、Myf-5和Myf-6(也被称为MRF-4或herculin)。有趣的是,大多数肌肉细胞在稳定状态下表达Myo D或Myf-5,但当诱导其分化时,所有细胞都会启动肌细胞生成素的表达。Myo D转录因子与表达更广泛的bHLH基因家族(E家族)的产物形成异二聚体,该家族至少包含三个不同的基因:E2A、IF2和HEB。Myo D-E异二聚体强烈结合到共识(CANNTG)E盒靶位点,这些靶位点是许多肌肉特异性终末分化基因上游调控序列中的功能重要元件。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

myogenin 抗体 (5FD)

sc-52903
200 µg/ml
$316.00

myogenin (5FD): m-IgG Fc BP-HRP 套装

sc-525567
200 µg Ab; 10 µg BP
$354.00

myogenin 抗体 (5FD) HRP

sc-52903 HRP
200 µg/ml
$316.00

myogenin 抗体 (5FD) FITC

sc-52903 FITC
200 µg/ml
$330.00

myogenin 抗体 (5FD) PE

sc-52903 PE
200 µg/ml
$343.00

myogenin 抗体 (5FD) Alexa Fluor® 488

sc-52903 AF488
200 µg/ml
$357.00

myogenin 抗体 (5FD) Alexa Fluor® 647

sc-52903 AF647
200 µg/ml
$357.00

I read some papers in which the M-225 was successively used in zebrafish but in your site it is stated it has been discontinued and replaced with the sc-52903. Is it the same antibody? Do you know if this antibody works with zebrafish? I thank you

Asked by: Luciaaidos
Thank you for your question. myogenin Antibody (5FD): sc-52903 is a fundamentally different antibody and is only recommended for detection of myogenin of mouse, rat and human origin by WB, IP, IF and IHC(P) based on our tests with the product. We do not have experimental data determining viability in fish species.
Answered by: Tech Support Europe
Date published: 2024-03-28

Can you recommend a positive control to use with sc-52903: myogenin (5FD) for Western blot?

Asked by: jenniferc
We recommend SJRH30: sc-2287 or RD: sc-364791 whole cell lysates as we have great Western blot data showing specific detection of myogenin in these cell lines.
Answered by: Technical Support 7
Date published: 2017-01-10
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Rated 5 out of 5 by from Good result in IFWe tested this antibody in IF at 1:100, with antigen retrieval, on paraffin sections of rainbow trout skeletal muscle with excellent results. The positive control was pig skeletal muscle.
Date published: 2024-09-12
Rated 4 out of 5 by from band in the right sizeI got this sample and tried it using western blot and it working BUT a second higher band was there too
Date published: 2018-03-15
Rated 5 out of 5 by from Worked very well for western blottingReceived a free sample of this antibody a month back to test on differentiated L6 cells. The antibody worked very well at a 1:200 dilution in 5% milk in TBS for western blotting using 30ug of cell lysate per well.
Date published: 2017-03-06
Rated 4 out of 5 by from C2C12 experimentwe detected the band in Western blot analysis. sensitive not very good
Date published: 2017-02-20
Rated 4 out of 5 by from specific but cross-reacts other muscle proteinsMyogenin antibody is specific for myogenin but also cross-reacts with a number of other muscle proteins
Date published: 2017-02-19
Rated 5 out of 5 by from Highly specific, great antbodyI first tried free sample of this antibody and was happy with the result. Then I ordered it. Single band in Western blot. Love it.
Date published: 2017-01-31
Rated 5 out of 5 by from very goodthe antibody is very specific. the band is very strong! worth to use it!
Date published: 2017-01-05
Rated 5 out of 5 by from Versatile antibody!This antibody works great for a number of applications with verification in multiple publications for Western blot and IHC.
Date published: 2016-12-16
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myogenin 抗体 (5FD) is rated 4.8 out of 5 by 14.
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