Date published: 2025-9-6
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MyoD Antibody (G-1) - Western Blotting - Image 380062
MyoD 抗体 (G-1): sc-377460. HeLa (A), HL-60 (B), SJRH30 (C), NIH/3T3 (D) 和 RD (E) 全细胞裂解液及 A-673 核提取物 (F) 中 MyoD 表达的 Western 印迹分析.
MyoD 抗体 (G-1): sc-377460. HeLa (A), HL-60 (B), SJRH30 (C), NIH/3T3 (D) 和 RD (E) 全细胞裂解液及 A-673 核提取物 (F) 中 MyoD 表达的 Western 印迹分析.

MyoD 抗体 (G-1): sc-377460

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说明书
  • MyoD 抗体 G-1 是小鼠单克隆 IgG2b κ，MyoD抗体, 在144篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的MyoD的氨基酸1-318
  • MyoD 抗体 (G-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MyoD
  • 抗MyoD抗体（G-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-377460 X, 200 µg/0.1 ml)
  • 关于如何获取MyoD (G-1): sc-377460的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 MyoD 抗体 (G-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MyoD 抗体 (G-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

MyoD抗体（G-1）是一种IgG2b κ小鼠单克隆MyoD抗体（也称为MYOD1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的MyoD蛋白。MyoD抗体（G-1）既可以是非偶联的抗MyoD抗体形式，也可以是多种偶联形式的抗MyoD抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。成肌细胞的分化受多种正负作用因子的调控。一个已知在调节肌肉细胞发育中起重要作用的特征明确的螺旋-环-螺旋（HLH）蛋白质家族包括MyoD、成肌细胞生成素、Myf-5和Myf-6（也称为MRF-4或herculin）。值得注意的是，在分化状态下，大多数肌肉细胞表达MyoD或Myf-5，但当被诱导分化时，所有细胞都会开启肌细胞生成素的表达。MyoD转录因子与更广泛表达的bHLH基因家族（E家族）的产物形成异二聚体，该家族至少由三个不同的基因组成：E2A、IF2和HEB。MyoD-E异二聚体与共识（CANNTG）E盒靶向位点紧密结合，这些位点是许多肌肉特异性终末分化基因上游调控序列中的功能重要元件。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MyoD 抗体 (G-1) 参考文献:

  1. 细胞周期调节因子与骨骼肌成纤维细胞中肌源性因子MyoD之间的相互作用。  |  Kitzmann, M. and Fernandez, A. 2001. Cell Mol Life Sci. 58: 571-9. PMID: 11361092
  2. [对MyoD调控的新见解：参与横纹肌肉瘤途径？  |  Tintignac, LA., et al. 2001. Bull Cancer. 88: 545-8. PMID: 11459700
  3. MyoD与E蛋白HEB beta协同诱导肌分化。  |  Parker, MH., et al. 2006. Mol Cell Biol. 26: 5771-83. PMID: 16847330
  4. MyoD在去神经、废用和锻炼肌肉中的作用。  |  Legerlotz, K. and Smith, HK. 2008. Muscle Nerve. 38: 1087-100. PMID: 18642380
  5. MyoD是髓母细胞瘤中的肿瘤抑制基因。  |  Dey, J., et al. 2013. Cancer Res. 73: 6828-37. PMID: 24092238
  6. 组蛋白去甲基化酶KDM4B与MyoD相互作用，调节C2C12成肌细胞中的肌源性分化。  |  Choi, JH., et al. 2015. Biochem Biophys Res Commun. 456: 872-8. PMID: 25534856
  7. PRMT1 通过 MyoD R121 处的精氨酸甲基化激活肌生成素转录。  |  Liu, Q., et al. 2019. Biochim Biophys Acta Gene Regul Mech. 1862: 194442. PMID: 31634638
  8. FOXP1 与 MyoD 相互作用，抑制其转录和肌细胞转化  |  Wright, WE., et al. 2021. J Cell Signal. 2: 9-26. PMID: 33554216
  9. 生成MyoD敲入报告小鼠系，研究肌肉干细胞的动态和异质性。  |  Fujita, R., et al. 2023. iScience. 26: 106592. PMID: 37250337
  10. MyoD和c-fos的表达。  |  Walsh, K. 1993. Nature. 365: 611-2. PMID: 8413625

订购信息

产品名称产品编号规格价格数量收藏夹

MyoD 抗体 (G-1)

sc-377460
200 µg/ml
$316.00

MyoD (G-1): m-IgGκ BP-HRP 套装

sc-523335
200 µg Ab, 40 µg BP
$354.00

MyoD (G-1): m-IgG2b BP-HRP 套装

sc-549109
200 µg Ab; 10 µg BP
$354.00

MyoD 抗体 (G-1) X

sc-377460 X
200 µg/0.1 ml
$316.00

MyoD 抗体 (G-1) AC

sc-377460 AC
500 µg/ml, 25% agarose
$416.00

MyoD 抗体 (G-1) HRP

sc-377460 HRP
200 µg/ml
$316.00

MyoD 抗体 (G-1) FITC

sc-377460 FITC
200 µg/ml
$330.00

MyoD 抗体 (G-1) PE

sc-377460 PE
200 µg/ml
$343.00

MyoD 抗体 (G-1) Alexa Fluor® 488

sc-377460 AF488
200 µg/ml
$357.00

MyoD 抗体 (G-1) Alexa Fluor® 546

sc-377460 AF546
200 µg/ml
$357.00

MyoD 抗体 (G-1) Alexa Fluor® 594

sc-377460 AF594
200 µg/ml
$357.00

MyoD 抗体 (G-1) Alexa Fluor® 647

sc-377460 AF647
200 µg/ml
$357.00

MyoD 抗体 (G-1) Alexa Fluor® 680

sc-377460 AF680
200 µg/ml
$357.00

MyoD 抗体 (G-1) Alexa Fluor® 790

sc-377460 AF790
200 µg/ml
$357.00

I am using the MyoD antibody (G-1, sc-377460) and need to confirm the exact epitope it recognizes. My protein is a truncated MYOD1 (from Cys101 to C-terminus with an N-terminal His-tag). Could you clarify if this antibody binds to the C-terminal region？

Asked by: crazy emsa
Thank you for your question. MyoD Antibody (G-1): sc-377460 was raised against amino acids 1-318 of MyoD of mouse origin, representing full-length protein. No epitope mapping has been performed for this antibody as of yet, so its exact binding site is not known at this point in time.
Answered by: Tech Support Europe
Date published: 2025-07-01

Can sc-377460 recognize bovine MyoD in Western blot analysis and IHC?

Asked by: M Kobayashi
I tried this antibody (x1000) to detect recombinant bovine MyoD expressed in 293T cells and native MyoD in differentiating C2C12 cells by Western analysis. Approximately 45 kDa protein was clearly detected in 293T cells transfected with MyoD-expressing vector and differentiating C2C12 cells. However, a strong non-specific signal (~50 kDa) with some fainter bands were also detected in both cell line. Therefore, I would like to conclude that this antibody can detect bovine MyoD in the Western blot analysis. M Kobayashi
Answered by: M Kobayashi
Date published: 2025-04-24

Is there a preferred way to fix C2C12 cells to use the Anti-MyoD Antibody (G-1) Alexa Fluor® 546?

Asked by: JEFZ
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-12-03

いつもお世話になります。 御社の下記の抗体を使用しています。 MyoD (G-1)  データシート等に掲載されているWBの予測分子量は約45kDaです。 しかしながら、この45kDaに加え、約70kDa近辺に強いバンドを検出しています。 我々の結果の場合、45kDaより70kDaのバンドの方が強く検出されます。 御社のホームページ上でも70kDa近辺に強いバンドが検出されているデータが掲載されています。 この70kDaのバンドの由来に関する情報をお持ちでしたら、ご教示ください。

Asked by: Shuji
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

您好，我想问一下，我买的myod的抗体，应该放在四度的，但是放在-20度冰箱24个小时了，请问会有影响吗？十分着急！麻烦你帮我解答一下谢谢！

Asked by: 诸葛钢铁
感谢您的提问。MyoD 抗体 (G-1): sc-377460 应该保存在4°C，不能冷冻。冷冻后对产品的质量可能会造成不良影响。 Thank you for your question. MyoD Antibody (G-1): sc-377460 should be stored at 4 °C. Do NOT FREEZE. The Quality of the product may be affected after freezing.
Answered by: Technical Support 12
Date published: 2021-07-07

I had used MyoD C-20 to detect MyoD in chicken myoblasts. It was great. However, C-20 was discontiuned and MyoD G-1 was produced instead. So, I brought the G-1, but the detection was not as good as C-20. Is there any way to get C-20?

Asked by: megimetel
Thank you for your question. Unfortunately, the rabbit polyclonal antibody, MyoD (C-20): sc-304, has been discontinued since 2016. It is no longer available for sale or distribution. We do not have any data or product citations yet using this mouse monoclonal antibody in chicken or avian samples. Please contact our Technical Service Department and we could assist you by performing sequence analysis to predict potential cross reactivity of our MyoD antibodies with chicken samples.
Answered by: Technical Support
Date published: 2020-03-26

HI, I'm interested in detecting MYOD in bovine cells . do you know if it cross rects? thanks

Asked by: Anonymous
Thank you for your question. This antibody hasn’t been tested in bovine samples. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined, so we can not perform sequence analysis at this time to predict potential cross reactivity with that species.
Answered by: Tech Service
Date published: 2019-06-28

Buenos días, estoy haciendo una inmunoprecipitacion con MyoD y quiero ver su anticuerpo HDAC1, pero la banda que me sale a 60 kDa no tengo claro si es HDAC1 o es la IgG, podrian decirme el peso al que me deberian salir las IgG??

Asked by: Anonymous
Muchas gracias por su pregunta. El peso molecular de la IgG es de 150 kDa, la cadena pesada de aprox 50 kDa mientras la ligera de aprox 25 kDa. Si está realizando otro método de detección, puede encontrar todos nuestros protocolos en: https://www.scbt.com/scbt/es/resources/protocols. Si necesita más información, no dude en contactar con nosotros: spain@scbt.com
Answered by: Technical Support Europe
Date published: 2019-02-27
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Rated 1 out of 5 by from Nuclear staining in all cellsIn my hand this antibody stains all cells in the lymph node. No specificity at all. I've tried dilution in the range 1:50 to 1:1000 with different physical antigen retrival using different buffers.
Date published: 2023-01-23
Rated 5 out of 5 by from Good antibodyWe purchased this antibody for immunostainning and western blot. It works very well for both the applications in cultured myoblasts.
Date published: 2018-05-06
Rated 4 out of 5 by from good abI got a free sample from SCBT, and i tested it on primary satellite cell at 1/50. it worked well for Immunofluorescence.
Date published: 2017-10-13
Rated 5 out of 5 by from Great for pull down assays.Got this antibody for my immunoprecipitation project for human samples. Works great. Western blotting with MyoD western antibody shows clear double bands below 50KD IgG heavy chain band which is commonly detect in human samples. Decided to move on our work with this antibody.
Date published: 2017-09-29
Rated 5 out of 5 by from Great productWe used this product for immunostaining in satellite cells. Works well for us.
Date published: 2017-09-27
Rated 5 out of 5 by from Great AntibodyI received the free trial and found that this antibody was very specific for the primary myoblast cultures I am working with. Fixed cells with methanol at -20C for 5 minutes and obtained great images.
Date published: 2017-08-03
Rated 4 out of 5 by from DearI bought it a mouth ago.I think it is very well.The concentration of MyoD is 1：1000.
Date published: 2017-07-05
Rated 4 out of 5 by from It is okUsed at 1:1000 in C2C12 cells. It is ok. There are also some non specific bands there.
Date published: 2017-06-02
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MyoD 抗体 (G-1) is rated 4.6 out of 5 by 20.
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