MMAB Double Nickase Plasmid (h): sc-409091-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MMAB Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MMAB Double Nickase Plasmid (h) and MMAB Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MMAB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MMAB Antibody (G-3): sc-271424
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MMAB Double Nickase Plasmid (h) | sc-409091-NIC | 20 µg | $410.00 | |||
MMAB Double Nickase Plasmid (h2) | sc-409091-NIC-2 | 20 µg | $410.00 |
MMAB encodes an adenosyltransferase required for intracellular vitamin B12 (cobalamin) metabolism, catalyzing conversion of cobalamin to adenosylcobalamin, the essential cofactor for methylmalonyl-CoA mutase in mitochondrial propionate catabolism. Through this pathway, MMAB supports efficient flux from methylmalonyl-CoA to succinyl-CoA, linking amino acid and odd-chain fatty acid breakdown to the tricarboxylic acid cycle. Loss of MMAB function disrupts cobalamin-dependent mitochondrial metabolism and is associated with methylmalonic acidemia and related inborn errors of metabolism characterized by methylmalonate accumulation. MMAB therefore serves as a key node for studying mitochondrial cofactor biogenesis, metabolic stress responses, and genotype–phenotype relationships in cobalamin pathway defects.
MMAB Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MMAB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MMAB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MMAB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MMAB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.