Date published: 2025-9-6
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MLH1 Antibody (B-12) - Western Blotting - Image 350804
MLH1 抗体 (B-12): sc-271978. KNRK 核提取物 (A) 和 SW480 (B), MDA-MB-231 (C), WI-38 (D), NTERA-2 cl. D1 (E) 和 TT (F) 全细胞裂解液中 MLH1 表达的 Western 印迹分析.
MLH1 抗体 (B-12): sc-271978. KNRK 核提取物 (A) 和 SW480 (B), MDA-MB-231 (C), WI-38 (D), NTERA-2 cl. D1 (E) 和 TT (F) 全细胞裂解液中 MLH1 表达的 Western 印迹分析.

MLH1 抗体 (B-12): sc-271978

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寫評論提問

说明书
  • MLH1 抗体 B-12 是小鼠单克隆 IgG1 κ，MLH1抗体, 在7篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的MLH1的C-terminus附近的氨基酸737-756之间
  • MLH1 抗体 (B-12) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的MLH1; 也和以下物种反应，包括： 和 canine, bovine, porcine and avian
  • 抗MLH1抗体（B-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MLH1 (B-12): sc-271978的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是MLH1 Antibody (B-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MLH1 Antibody (B-12) 打包提供（请参阅下面的订购信息）。

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描述
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说明书与实验方案
研究信息

関連項目

额外MLH抗体，包括MLH3

MLH1抗体（B-12）是一种IgG1 κ小鼠单克隆MLH1抗体（也称为DNA错配修复蛋白Mlh1抗体、COCA2抗体、HNPCC抗体、FCC2抗体、MutL同源物1结肠癌非息肉病2型（E. Coli）抗体、结肠癌非息肉病2型抗体、MutL蛋白同源物1抗体或HML抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的MLH1蛋白。MLH1抗体（B-12）既可以是非偶联的抗MLH1抗体形式，也可以是多种偶联形式的抗MLH1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。DNA错配修复（MMR）是维持遗传稳定性的重要过程。功能性DNA错配修复途径的缺失是多种不同类型人类癌症的共同特征，这可能是由于MMR基因突变或启动子甲基化基因沉默所导致。MLH1是负责错配修复的蛋白质复合物的重要组成部分，在淋巴细胞、心脏、结肠、乳腺、肺、脾脏、睾丸、前列腺、甲状腺和胆囊中表达，并在多种卵巢肿瘤中发生甲基化。MLH1蛋白表达的丧失与突变表型、微卫星不稳定性和癌症易感性相关。在遗传性非息肉病性结直肠癌（HNPCC）中，这是一种常染色体显性遗传性癌症综合征，标志着结直肠癌和其他多种类型癌症的高风险，MLH1基因表现出致病性突变。某些癌细胞系，包括白血病CCRF-CEM、结肠HCT 116和KM12以及卵巢癌SK-OV-3和IGROV-1，完全缺乏MLH1的表达，而MLH1在60%的黑色素瘤、70%的非侵袭性鳞状细胞癌和30%的侵袭性鳞状细胞癌中有表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MLH1 抗体 (B-12) 参考文献:

  1. 子宫颈浸润性和原位鳞状细胞癌中 Msh2, Mlh1, Fhit, p53, Bcl-2 和 Bax 的表达。  |  Giarnieri, E., et al. 2000. Clin Cancer Res. 6: 3600-6. PMID: 10999751
  2. 监测 DNA 错配修复基因突变携带者。  |  Järvinen, HJ. and Aarnio, M. 2000. Ann Chir Gynaecol. 89: 207-10. PMID: 11079789
  3. NCI 抗癌药物筛选细胞系中 MLH1 和 MSH2 DNA 错配修复蛋白的特征。  |  Taverna, P., et al. 2000. Cancer Chemother Pharmacol. 46: 507-16. PMID: 11138465
  4. 分析恶性黑色素瘤中 DNA 错配修复蛋白的表达。  |  Korabiowska, M., et al. 2000. Anticancer Res. 20: 4499-505. PMID: 11205295
  5. MSH2种系突变的Muir-Torre患者皮脂腺肿瘤的 '第二次打击'：等位基因缺失不是首选的失活模式。  |  Kruse, R., et al. 2001. J Invest Dermatol. 116: 463-5. PMID: 11231323
  6. 原发性卵巢癌显示出涉及已知肿瘤抑制基因的多种甲基化表型。  |  Strathdee, G., et al. 2001. Am J Pathol. 158: 1121-7. PMID: 11238060
  7. 黑色素瘤淋巴结和肝转移灶细胞学标本中 DNA 倍性状态与 DNA 错配修复基因 MLH1 和 MSH2 表达的关系。  |  Korabiowska, M., et al. 2001. Diagn Cytopathol. 24: 157-62. PMID: 11241897
  8. 哺乳动物 MLH1 参与过氧化物诱导的氧化应激的凋亡反应  |  Hardman, RA., et al. 2001. Cancer Res. 61: 1392-7. PMID: 11245440
  9. 解读错义变异：比较人类疾病基因 CDKN2A, MLH1, MSH2, MECP2 和酪氨酸酶 (TYR) 的计算方法。  |  Chan, PA., et al. 2007. Hum Mutat. 28: 683-93. PMID: 17370310
  10. 恒河猴MLH1-rheMac遗传性非息肉病性结直肠癌综合征。  |  Brammer, DW., et al. 2018. Proc Natl Acad Sci U S A. 115: 2806-2811. PMID: 29490919
  11. MLH1 缺乏会导致线粒体代谢紊乱。  |  Rashid, S., et al. 2019. Cell Death Dis. 10: 795. PMID: 31641109

订购信息

产品名称产品编号规格价格数量收藏夹

MLH1 抗体 (B-12)

sc-271978
200 µg/ml
$316.00

MLH1 (B-12): m-IgG Fc BP-HRP 套装

sc-529295
200 µg Ab; 10 µg BP
$354.00

MLH1 (B-12): m-IgGκ BP-HRP 套装

sc-522112
200 µg Ab, 40 µg BP
$354.00

MLH1 (B-12): m-IgG1 BP-HRP 套装

sc-543435
200 µg Ab; 20 µg BP
$354.00

MLH1 抗体 (B-12) AC

sc-271978 AC
500 µg/ml, 25% agarose
$416.00

MLH1 抗体 (B-12) HRP

sc-271978 HRP
200 µg/ml
$316.00

MLH1 抗体 (B-12) FITC

sc-271978 FITC
200 µg/ml
$330.00

MLH1 抗体 (B-12) PE

sc-271978 PE
200 µg/ml
$343.00

MLH1 抗体 (B-12) Alexa Fluor® 488

sc-271978 AF488
200 µg/ml
$357.00

MLH1 抗体 (B-12) Alexa Fluor® 546

sc-271978 AF546
200 µg/ml
$357.00

MLH1 抗体 (B-12) Alexa Fluor® 594

sc-271978 AF594
200 µg/ml
$357.00

MLH1 抗体 (B-12) Alexa Fluor® 647

sc-271978 AF647
200 µg/ml
$357.00

MLH1 抗体 (B-12) Alexa Fluor® 680

sc-271978 AF680
200 µg/ml
$357.00

MLH1 抗体 (B-12) Alexa Fluor® 790

sc-271978 AF790
200 µg/ml
$357.00

MLH1 (B-12) 中和勝肽

sc-271978 P
100 µg/0.5 ml
$68.00

I would like to map MLH1 sites in the pig genome by ChIP-Seq in pig (Sus scrofa) spermatocytes and wonder whether this antibody will be suitable for this technology and species. Thanks in advance

Asked by: Alex Clop
Thank you for your question. Anti-MLH1 Antibody (B-12): sc-271978 is recommended for detection of MLH1 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian. It has not been tested for ChIP-Seq applications in particular, but the agarose conjugate sc-271978 AC is suitable for pulldown experiments.
Answered by: Tech Support Europe
Date published: 2021-09-15

What application is the blocking peptide sc-271978 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from LOOKS GOODI want to use this antibody in my study. I know this antidody from article because of its perfect performance
Date published: 2020-11-20
Rated 1 out of 5 by from Use for staining for chromosome spreadI tested it for immunofluorescence staining on mouse spermatocyte surface spread and expected for a focus staining on crossover site. Unfortunately, it seems not working for this specific application in my hands. Not working， not suitable. Too hard
Date published: 2020-09-21
Rated 4 out of 5 by from Worked wellWe used MLH1 in immunofluorescence at 1:100 dilution. It the nuclear staining was clear in paraformaldehyde fixed cells.
Date published: 2019-10-20
Rated 5 out of 5 by from Worked in Western blot10ug protein from Hek293 cells were tested in western blot, Block:5%milk in PBST, Primary antibody 1/100 in 1% milk PBST, cold room overnight secondary antibody,1/5000 anti-mouse HRP in 1% milk PBST ECL dura substrate 5 min develop in Bio-rad geldoc
Date published: 2017-11-29
Rated 5 out of 5 by from perfect1:1000 for WB in DU145、PC3 cell,the band is clear and have no background
Date published: 2017-09-23
Rated 3 out of 5 by from Not working for meiotic crossover markVery glad to get this sample antibody for free from SCBT for our application test. I tested it for immunofluorescence staining on mouse spermatocyte surface spread and expected for a focus staining on crossover site. Unfortunately, it seems not working for this specific application in my hands. Anyway, big thanks to SCBT staff.
Date published: 2017-09-14
Rated 5 out of 5 by from MLH1 antibody in Western BlotAccurately detected cell clones with a CRISPR-mediated gene deletion.
Date published: 2017-07-24
Rated 5 out of 5 by from easy operated antibodyWe study MLH1 expression and cell proliferation in cervical cancer use this antibdoy.It a good quality and very effective ,we obtained the target band 85kda very easily by WB.
Date published: 2017-07-15
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MLH1 抗体 (B-12) is rated 4.3 out of 5 by 15.
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