Date published: 2025-9-7
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MKP-2 Antibody (F-10) - Western Blotting - Image 2328
MKP-2 抗体 (F-10): sc-17821. Hela + UV 全细胞裂解液中 MKP-2 表达的 Western 印迹分析.
MKP-2 Antibody (F-10) - Western Blotting - Image 2328
MKP-2 抗体 (F-10): sc-17821. Hela + UV 全细胞裂解液中 MKP-2 表达的 Western 印迹分析.
MKP-2 抗体 (F-10): sc-17821. Hela + UV 全细胞裂解液中 MKP-2 表达的 Western 印迹分析.

MKP-2 抗体 (F-10): sc-17821

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说明书
  • MKP-2 抗体 F-10 是小鼠单克隆 IgG2a κ，MKP-2抗体, 在4篇文献中引用，规格为200 µg/ml
  • 针对氨基酸78-144的抗原，靠近N-terminus
  • MKP-2 抗体 (F-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 MKP-2; 也和以下物种反应，包括： 和 equine, bovine and porcine
  • 抗MKP-2抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MKP-2 (F-10): sc-17821的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是MKP-2 Antibody (F-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MKP-2 Antibody (F-10) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外MKP抗体，包括MKP-1, MKP-2, MKP-3, MKP-4, MKP-5和MKP-6

MKP-2抗体（F-10）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的MKP-2蛋白。MKP-2 (F-10) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。MKP-2，也称为MAP激酶磷酸酶2或双特异性蛋白磷酸酶4（DUSP4），在调节促分裂原活化蛋白激酶（MAPK）信号通路中起着关键作用，该信号通路对于细胞生长、分化、凋亡等多种细胞过程至关重要。MKP-2主要位于细胞质中，作为双特异性磷酸酶，它能够特异性地脱除MAP激酶ERK1和ERK2上的苏氨酸和酪氨酸残基。这种定位至关重要，因为MKP-2能够有效调节激酶活性，从而影响细胞对压力和生长因子的反应。MKP-2受转录因子E2F-1调控，E2F-1与MKP-2启动子结合并增强其表达，从而将MKP-2与细胞周期调控和细胞凋亡相关通路联系起来。MKP-2能够微调MAPK信号，这凸显了其在癌症治疗中作为治疗靶标的潜力，因为该通路的失调通常与肿瘤发生有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MKP-2 抗体 (F-10) 参考文献:

  1. 丝裂原活化蛋白激酶磷酸酶 2：p53 在细胞凋亡中的新转录靶标  |  Shen, WH., et al. 2006. Cancer Res. 66: 6033-9. PMID: 16778175
  2. 利用氢/氘交换质谱法绘制 ERK2-MKP3 结合界面图。  |  Zhou, B., et al. 2006. J Biol Chem. 281: 38834-44. PMID: 17046812
  3. 核ERK信号调节二倍体人类细胞的复制衰老。  |  Tresini, M., et al. 2007. J Biol Chem. 282: 4136-51. PMID: 17145763
  4. HoxA10 可激活骨髓细胞中编码丝裂原活化蛋白激酶磷酸酶 2（Mkp2）基因的转录。  |  Wang, H., et al. 2007. J Biol Chem. 282: 16164-76. PMID: 17430893
  5. E2F-1 与 MAPK 级联之间的分子联系。  |  Wang, J., et al. 2007. J Biol Chem. 282: 18521-18531. PMID: 17452331
  6. 在体节分割钟中，Hes7摆动的引发和传播受Fgf和notch信号的协同调控。  |  Niwa, Y., et al. 2007. Dev Cell. 13: 298-304. PMID: 17681139
  7. 在对氧化应激的信号反应中，几种双重特异性磷酸酶协调控制着 JNK 激活的程度和持续时间。  |  Teng, CH., et al. 2007. J Biol Chem. 282: 28395-28407. PMID: 17681939
  8. 促分裂原活化蛋白激酶磷酸酶2（MKP-2）调节急性肺损伤的早期炎症。  |  Cornell, TT., et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303: L251-8. PMID: 22683570
  9. 乳腺癌对他莫昔芬敏感性的MKP-1和MKP-2功能分析。  |  Haagenson, KK., et al. 2014. Oncotarget. 5: 1101-10. PMID: 24658355
  10. MKP2通过JNK依赖性途径抑制TGF-β1诱导的肾小管上皮细胞上皮-间质转化。  |  Li, Z., et al. 2018. Clin Sci (Lond). 132: 2339-2355. PMID: 30322849
  11. MKP2通过抑制JNK抑制TGF-β1诱导的上皮-间质转化。  |  Loeffler, I. 2019. Clin Sci (Lond). 133: 545-550. PMID: 30760641
  12. 肥胖症中MKP-2上调的代谢影响促进胰岛素抵抗和脂肪肝疾病。  |  Fernando, S., et al. 2022. Nutrients. 14: PMID: 35745205

订购信息

产品名称产品编号规格价格数量收藏夹

MKP-2 抗体 (F-10)

sc-17821
200 µg/ml
$316.00

MKP-2 (F-10): m-IgG Fc BP-HRP 套装

sc-525490
200 µg Ab; 10 µg BP
$354.00

MKP-2 (F-10): m-IgG2a BP-HRP 套装

sc-546285
200 µg Ab; 10 µg BP
$354.00

MKP-2 抗体 (F-10) AC

sc-17821 AC
500 µg/ml, 25% agarose
$416.00

MKP-2 抗体 (F-10) HRP

sc-17821 HRP
200 µg/ml
$316.00

MKP-2 抗体 (F-10) FITC

sc-17821 FITC
200 µg/ml
$330.00

MKP-2 抗体 (F-10) PE

sc-17821 PE
200 µg/ml
$343.00

MKP-2 抗体 (F-10) Alexa Fluor® 488

sc-17821 AF488
200 µg/ml
$357.00

MKP-2 抗体 (F-10) Alexa Fluor® 546

sc-17821 AF546
200 µg/ml
$357.00

MKP-2 抗体 (F-10) Alexa Fluor® 594

sc-17821 AF594
200 µg/ml
$357.00

MKP-2 抗体 (F-10) Alexa Fluor® 647

sc-17821 AF647
200 µg/ml
$357.00

MKP-2 抗体 (F-10) Alexa Fluor® 680

sc-17821 AF680
200 µg/ml
$357.00

MKP-2 抗体 (F-10) Alexa Fluor® 790

sc-17821 AF790
200 µg/ml
$357.00

Are there any issues with using sc-17821: MKP-2 (F-10) mouse monoclonal primary antibody on mouse samples?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-17821 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 3 out of 5 by from Good antibodyI tried this antibody on two different melanoma cell lines lysates (A375 and Sk-Mel-5). When i used it the first time at a concentration of 1:1000 i obtained a band at the predicted molecular weight but then the antobody deteriorate pretty fast
Date published: 2022-11-22
Rated 4 out of 5 by from Good for IFThis MKP-2 Antibody (F-10), sc-17821, shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2018-02-20
Rated 1 out of 5 by from NO bandsNo bands in Raw cell lysates (dilution 1:250) and a lot of background. The block step was performed with BSA 5% in TBS-T...
Date published: 2018-02-20
Rated 1 out of 5 by from No visible band in WBNo visible band in WB using 20 ug mouse cell lysates (1:200 dilution).
Date published: 2017-09-08
Rated 4 out of 5 by from work well in WBIn primary hepatocytes, we examined a plate between 55kd and 37kd using 1:100 dilution in WB.
Date published: 2017-06-20
Rated 1 out of 5 by from No band in Western using mouse cell lysatesWe failed to detect a band at the predicted size in a western blot using protein extracts of mouse cells.
Date published: 2017-03-09
Rated 5 out of 5 by from Excellent, versatile antibodyThe MKP-2 Antibody (F-10): sc-17821 works well for multiple applications including WB, IP, IF and ELISA. This antibody has also been cited several times for further verification
Date published: 2017-01-18
Rated 4 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. Appeared to be detection of the ~43 kDa target protein in a HeLa/Jurkat cell lysate mix, but not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
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MKP-2 抗体 (F-10) is rated 3.5 out of 5 by 12.
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