Date published: 2025-9-6

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Mfn1/Mitofusin 1 抗体 (D-10): sc-166644

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说明书
  • Mfn1/Mitofusin 1 抗体 D-10 是小鼠单克隆 IgG2a κ,Mfn1/Mitofusin 1抗体, 在114篇文献中引用,规格为200 µg/ml
  • 针对氨基酸10-74的抗原,在human起源的Mfn1的N-terminal细胞质域内
  • Mfn1/Mitofusin 1 抗体 (D-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Mitofusin 1
  • 抗Mfn1/Mitofusin 1抗体(D-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mfn1/Mitofusin 1 (D-10): sc-166644免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是Mfn1/Mitofusin 1 Antibody (D-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Mfn1/Mitofusin 1 Antibody (D-10) 打包提供(请参阅下面的订购信息)。

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関連項目

Mfn1抗体(D-10)是一种IgG2a κ小鼠单克隆Mfn1抗体(也被称为Mitofusin-1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Mfn1蛋白。Mfn1抗体(D-10)既有非偶联抗Mfn1抗体形式,也有多种偶联形式的抗Mfn1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Mitofusin 1(Mfn1)和mitofusin 2(Mfn2)是果蝇蛋白fuzzy onion(Fzo)的同源物。它们是线粒体膜蛋白,是线粒体融合的介质。Mfn蛋白的功能需要GTPase结构域,但GTPase依赖性反应的分子机制以及两种Mfn蛋白的功能划分尚不清楚。它们对于胚胎发育至关重要,并可能在肥胖症的病理生物学中发挥作用。尽管Mfn1和Mfn2基因广泛表达,但它们在不同组织中的表达水平不同。两种Mfn1转录本在心脏中升高,而Mfn2 mRNA在心脏和肌肉组织中大量表达,但在许多其他组织中仅低水平表达。Mfn1定位于线粒体,并以GTP依赖的方式参与至少两种不同的高分子量蛋白质复合物。纯化的重组Mfn1表现出比Mfn2高约八倍的GTPase活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mfn1/Mitofusin 1 抗体 (D-10) 参考文献:

  1. 人类丝裂蛋白对线粒体形态的控制  |  Santel, A. and Fuller, MT. 2001. J Cell Sci. 114: 867-74. PMID: 11181170
  2. 哺乳动物中无处不在的跨膜 GTP 酶 Fzo 的同源物 mitofusins 的膜拓扑结构和线粒体靶向。  |  Rojo, M., et al. 2002. J Cell Sci. 115: 1663-74. PMID: 11950885
  3. 丝裂蛋白Mfn1和Mfn2协调调节线粒体融合,对胚胎发育至关重要。  |  Chen, H., et al. 2003. J Cell Biol. 160: 189-200. PMID: 12527753
  4. Mitofusin-2决定线粒体网络结构和线粒体代谢。肥胖症改变了一种新的调节机制。  |  Bach, D., et al. 2003. J Biol Chem. 278: 17190-7. PMID: 12598526
  5. Mitofusin-1 蛋白是哺乳动物细胞中一种普遍表达的线粒体融合介质。  |  Santel, A., et al. 2003. J Cell Sci. 116: 2763-74. PMID: 12759376
  6. Mitofusin 1 和 2 通过 GTPase 活性在线粒体融合反应中发挥着不同的作用。  |  Ishihara, N., et al. 2004. J Cell Sci. 117: 6535-46. PMID: 15572413
  7. 神经支配通过miR-142a-5p/MFN1轴驱动骨骼肌萎缩并诱导线粒体功能障碍、线粒体自噬和细胞凋亡。  |  Yang, X., et al. 2020. Theranostics. 10: 1415-1432. PMID: 31938072
  8. lncRNA Malat1通过miR-26b-5p/Mfn1轴介导的线粒体动力学在小鼠心肌梗死后调节微血管功能。  |  Chen, Y., et al. 2021. Redox Biol. 41: 101910. PMID: 33667993
  9. STING1通过MFN1/2依赖的线粒体融合促进铁死亡。  |  Li, C., et al. 2021. Front Cell Dev Biol. 9: 698679. PMID: 34195205
  10. FUNDC2通过抑制MFN1介导的线粒体融合促进肝癌的发生。  |  Li, S., et al. 2022. Nat Commun. 13: 3486. PMID: 35710796
  11. 恩格列净靶向Mfn1和Opa1,以减轻视网膜缺血和再灌注损伤中小胶质细胞介导的神经炎症。  |  Yang, Z., et al. 2023. J Neuroinflammation. 20: 296. PMID: 38082266

订购信息

产品名称产品编号规格价格数量收藏夹

Mfn1/Mitofusin 1 抗体 (D-10)

sc-166644
200 µg/ml
$316.00

Mfn1/Mitofusin 1 (D-10): m-IgG Fc BP-HRP 套装

sc-529016
200 µg Ab; 10 µg BP
$354.00

Mfn1/Mitofusin 1 (D-10): m-IgGκ BP-HRP 套装

sc-521689
200 µg Ab, 40 µg BP
$354.00

Mfn1/Mitofusin 1 (D-10): m-IgG2a BP-HRP 套装

sc-547236
200 µg Ab; 10 µg BP
$354.00

Mfn1/Mitofusin 1 抗体 (D-10) AC

sc-166644 AC
500 µg/ml, 25% agarose
$416.00

Mfn1/Mitofusin 1 抗体 (D-10) HRP

sc-166644 HRP
200 µg/ml
$316.00

Mfn1/Mitofusin 1 抗体 (D-10) FITC

sc-166644 FITC
200 µg/ml
$330.00

Mfn1/Mitofusin 1 抗体 (D-10) PE

sc-166644 PE
200 µg/ml
$343.00

Mfn1/Mitofusin 1 抗体 (D-10) Alexa Fluor® 488

sc-166644 AF488
200 µg/ml
$357.00

Mfn1/Mitofusin 1 抗体 (D-10) Alexa Fluor® 546

sc-166644 AF546
200 µg/ml
$357.00

Mfn1/Mitofusin 1 抗体 (D-10) Alexa Fluor® 594

sc-166644 AF594
200 µg/ml
$357.00

Mfn1/Mitofusin 1 抗体 (D-10) Alexa Fluor® 647

sc-166644 AF647
200 µg/ml
$357.00

Mfn1/Mitofusin 1 抗体 (D-10) Alexa Fluor® 680

sc-166644 AF680
200 µg/ml
$357.00

Mfn1/Mitofusin 1 抗体 (D-10) Alexa Fluor® 790

sc-166644 AF790
200 µg/ml
$357.00

What is the best secondary to use with Mfn1 (D-10): sc-166644 monoclonal antibody in Western Blot?

Asked by: jenni
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-10
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Rated 1 out of 5 by from No bandsNo specific bands. Concentration of this antibody : 1/50. 10 ug of proteins
Date published: 2022-05-02
Rated 5 out of 5 by from It worked greatIt works great without background. The dilution I used was 1:500.
Date published: 2021-10-25
Rated 3 out of 5 by from We didn't get any bands.The sample was obtained from rat brain. The protein content is more than 50 micro-gram. The concentration of this antibody we used was 1:50. However, we didn't get any bands.
Date published: 2021-07-21
Rated 4 out of 5 by from Good antibody in WBThe antibody works as expected with PC12 whole cell lysates.
Date published: 2021-05-04
Rated 5 out of 5 by from 效果很好This antibody is easy to use, and the WB band is clear.
Date published: 2021-04-28
Rated 3 out of 5 by from okSingle weak band detected with western blot analysis
Date published: 2020-02-06
Rated 5 out of 5 by from The antibidy is very good.The antibidy is very good for western blot. It works very well.
Date published: 2017-09-28
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
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Mfn1/Mitofusin 1 抗体 (D-10) is rated 4.5 out of 5 by 31.
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