Date published: 2025-9-10

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MESP1 抗体 (JH.12): sc-130461

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说明书
  • MESP1 抗体 JH.12 是小鼠单克隆 IgG2a κ,MESP1抗体, 在5篇文献中引用,规格为100 µg/ml
  • 针对部分重组蛋白,其氨基酸序列在MESP1的human起源的1-63范围内
  • 推荐用于 mouse, rat 和 human 来源的MESP1 WB 和 IP检测
  • 关于如何获取MESP1 (JH.12): sc-130461免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是MESP1 Antibody (JH.12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MESP1 Antibody (JH.12) 打包提供(请参阅下面的订购信息)。

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関連項目

MESP1抗体(JH.12)是一种IgG2a κ型小鼠单克隆MESP1抗体(也称为MESP1抗体),可通过WB和IP检测小鼠、大鼠和人类来源的MESP1蛋白。MESP1抗体(JH.12)为非共轭形式的抗MESP1抗体。MESP1(中胚层后部1同源物),也称为bHLHc5,是一种含有268个氨基酸的蛋白质,包含一个碱性螺旋-环-螺旋(bHLH)结构域,该结构域可介导蛋白质二聚化,并能与DNA的E-box序列结合。MESP1定位于细胞核,作为转录因子,通过其bHLH结构域参与上皮化以及心脏和中胚层体节的发育。MESP1在原肠胚形成和体节形成过程中高度表达,对于心脏成熟过程中单一心脏管的形成至关重要。早期检测MESP1可能是发育中胚胎心脏前体细胞形成的指标。此外,MESP1在体节的头尾模式中发挥作用,该事件影响特定的Notch信号通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MESP1 抗体 (JH.12) 参考文献:

  1. MesP1 在心脏前体细胞中表达,是单个心脏管形成所必需的。  |  Saga, Y., et al. 1999. Development. 126: 3437-47. PMID: 10393122
  2. MesP1 和 MesP2 对心脏中胚层的发育至关重要。  |  Kitajima, S., et al. 2000. Development. 127: 3215-26. PMID: 10887078
  3. Mesp1 的表达是心血管发育的最早迹象。  |  Saga, Y., et al. 2000. Trends Cardiovasc Med. 10: 345-52. PMID: 11369261
  4. Mesp1 和 Mesp2 基因的转录调控:发育过程中增强子的不同用法。  |  Haraguchi, S., et al. 2001. Mech Dev. 108: 59-69. PMID: 11578861
  5. 突变的 MESP2 会导致人类脊柱骨发育不良。  |  Whittock, NV., et al. 2004. Am J Hum Genet. 74: 1249-54. PMID: 15122512
  6. MesP1 通过 Dkk-1 介导的 Wnt 信号阻断作用驱动脊椎动物的心血管分化。  |  David, R., et al. 2008. Nat Cell Biol. 10: 338-45. PMID: 18297060
  7. Mesp1 协调调控分化中的 ESCs 的心血管命运限制和上皮-间质转化。  |  Lindsley, RC., et al. 2008. Cell Stem Cell. 3: 55-68. PMID: 18593559
  8. Mesp1 是多能心血管祖细胞规范化的主调节器。  |  Bondue, A., et al. 2008. Cell Stem Cell. 3: 69-84. PMID: 18593560
  9. Mesp1 控制着早期心血管祖细胞时空模式化所必需的染色质和增强子景观。  |  Lin, X., et al. 2022. Nat Cell Biol. 24: 1114-1128. PMID: 35817961
  10. MESP1-RING1A 复合物在心脏分化中的重要作用  |  Liang, Q., et al. 2022. Dev Cell. 57: 2533-2549.e7. PMID: 36413948
  11. 早期心脏前体转录物和表观基因组规范中依赖 Mesp1 的发育断点  |  Krup, AL., et al. 2023. Development. 150: PMID: 36994838
  12. MesP1:一种在小鼠胃形成过程中表达于新生中胚层细胞的新型碱性螺旋-环-螺旋蛋白。  |  Saga, Y., et al. 1996. Development. 122: 2769-78. PMID: 8787751

订购信息

产品名称产品编号规格价格数量收藏夹

MESP1 抗体 (JH.12)

sc-130461
100 µg/ml
$333.00

MESP1 (JH.12): m-IgG Fc BP-HRP 套装

sc-537333
100 µg Ab; 10 µg BP
$354.00

MESP1 (JH.12): m-IgGκ BP-HRP 套装

sc-534574
100 µg Ab; 40 µg BP
$354.00

MESP1 (JH.12): m-IgG2a BP-HRP 套装

sc-547983
100 µg Ab; 10 µg BP
$354.00

I am using MESP1 (JH.12): sc-130461 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Good choice for immunofluorescenceThis antibody has been used for Immunofluorescence in published research. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Good antibodyI used 1 microliter for 10 ml and it worked very well thanks.
Date published: 2023-01-14
Rated 3 out of 5 by from Not too bad for IHCWe are continuing to work on the IHC with this antibody, but there first couple stains using buffer/heat antigen retrieval and amplification of the signal looks promising.
Date published: 2017-09-20
Rated 5 out of 5 by from excellentI have used this antibody sample in differentiation of embryonic stem cells,result shows that the antibody is very specific。
Date published: 2016-11-16
Rated 4 out of 5 by from ICC publishable data with human human pluripotentICC publishable data with human human pluripotent stem cells (hPSCs) - SCBT Publication Review
Date published: 2014-09-02
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MESP1 抗体 (JH.12) is rated 4.4 out of 5 by 5.
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