Date published: 2025-9-10
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Melanoma Marker Antibody (HMB45) - Western Blotting - Image 147497
黑色素瘤标记物抗体 (HMB45): sc-59305. Western 印迹分析 SK-MEL-28 全细胞裂解液中黑色素瘤标记物的表达.
Melanoma Marker Antibody (HMB45) - Western Blotting - Image 147497
黑色素瘤标记物抗体 (HMB45): sc-59305. Western 印迹分析 SK-MEL-28 全细胞裂解液中黑色素瘤标记物的表达.
Melanoma Marker Antibody (HMB45) - Western Blotting - Image 393169
黑色素瘤标记物抗体 (HMB45) Alexa Fluor® 790: sc-59305 AF790. 直接近红外 Western 印迹分析 A-431 全细胞裂解液中黑色素瘤标记物的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214. 用 Cruz Marker MW Tag-Alexa Fluor® 680 检测 Cruz Marker™ 分子量标准: sc-516730.
黑色素瘤标记物抗体 (HMB45): sc-59305. Western 印迹分析 SK-MEL-28 全细胞裂解液中黑色素瘤标记物的表达.

Melanoma Marker 抗体 (HMB45): sc-59305

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说明书
  • Melanoma Marker抗体(HMB45)是小鼠单克隆IgG1 κ, 在18篇文献中引用，规格为200 µg/ml
  • 针对提取物 pigmented melanoma metastases from lymph nodes human
  • 抗-Melanoma Marker 抗体 (HMB45) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes in junctional and blue nevus cells; 不会与 non-melanocytic cells产生交联反应
  • 抗Melanoma Marker抗体（HMB45）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Melanoma Marker (HMB45): sc-59305的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Melanoma Marker 抗体 (HMB45) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Melanoma Marker 抗体 (HMB45) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
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研究信息

関連項目

黑色素瘤标志物抗体（HMB45）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等应用检测小鼠、大鼠和人体样本中的黑色素瘤标志物。抗黑色素瘤标记物抗体（HMB45）可识别神经氨酸酶敏感的寡糖侧链，这种寡糖侧链存在于未成熟的黑色素体中，特别是在交界细胞和蓝痣细胞中。黑色素瘤标志物（HMB45）抗体在研究恶性黑色素瘤（一种由黑色素细胞引发的严重皮肤癌）的发展和恶化方面发挥着重要作用。黑色素瘤标记物单克隆抗体（HMB45）有助于研究人员了解影响细胞信号传递和肿瘤微环境中相互作用的复杂糖基化链。这些糖基化结构会影响黑色素瘤细胞逃避免疫反应和转移的方式，使它们成为治疗干预的重要靶点。抗黑色素瘤标志物抗体 (HMB45) 有非共轭型和与琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭的共轭型，可灵活满足不同的实验需求。鉴于黑色素瘤的发病率越来越高，而且与紫外线辐射照射有关，黑色素瘤标志物（HMB45）单克隆抗体是促进研究和开发有效治疗这种侵袭性癌症的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Melanoma Marker 抗体 (HMB45) 参考文献:

  1. 黑色素瘤患者外周血中酪氨酸酶和MART-1转录物的检测可重复性：使用实时定量RT-PCR的质量控制研究。  |  de Vries, TJ., et al. 1999. Br J Cancer. 80: 883-91. PMID: 10360670
  2. 恶性黑色素瘤标志物--黑色素皮质素 1 受体的组织分布和差异表达。  |  Salazar-Onfray, F., et al. 2002. Br J Cancer. 87: 414-22. PMID: 12177778
  3. 将血清左旋多巴/酪氨酸比率作为黑色素瘤标志物的评估。  |  Stoitchkov, K., et al. 2003. Melanoma Res. 13: 587-93. PMID: 14646622
  4. 黑色素瘤标记物碘苯甲酰胺的超结构细胞分布：提高 SIMS 成像在生命科学中的应用潜力。  |  Guerquin-Kern, JL., et al. 2004. Biomed Eng Online. 3: 10. PMID: 15068483
  5. 小脑桥脑角浸润性恶性黑色素瘤表皮样囊肿。  |  Meng, FG., et al. 2006. J Clin Neurosci. 13: 669-72. PMID: 16815016
  6. 黑色素瘤控制：答案不多，问题不少。  |  Dunbar, R., et al. 2006. N Z Med J. 119: U2172. PMID: 16998573
  7. 黑色素瘤的前哨节点活检。  |  Balch, CM. and Cascinelli, N. 2006. N Engl J Med. 355: 1370-1. PMID: 17005955
  8. 弥漫性大B细胞淋巴瘤的透明细胞变体：病例报告。  |  Manxhuka-Kerliu, S., et al. 2011. J Med Case Rep. 5: 182. PMID: 21569513
  9. 黑色素瘤标记物和乳腺癌  |  Lin, CS. 1990. Am J Clin Pathol. 94: 669-70. PMID: 2288610
  10. 黑色素瘤标记物（HMB-45）阳性的乳腺癌。正常乳腺和肿瘤性乳腺中的 HMB-45 免疫反应。  |  Bonetti, F., et al. 1989. Am J Clin Pathol. 92: 491-5. PMID: 2552794
  11. 诊断黑色素瘤的免疫组化和组织化学工具。  |  van Duinen, SG., et al. 1984. Cancer. 53: 1566-73. PMID: 6365305
  12. 皮肤恶性黑色素瘤的免疫组化分析：S-100 蛋白, HMB-45 单克隆抗体和 NKI/C3 单克隆抗体的比较。  |  Fernando, SS., et al. 1994. Pathology. 26: 16-9. PMID: 8165017

订购信息

产品名称产品编号规格价格数量收藏夹

Melanoma Marker 抗体 (HMB45)

sc-59305
200 µg/ml
$316.00

Melanoma Marker (HMB45): m-IgG Fc BP-HRP 套装

sc-526928
200 µg Ab; 10 µg BP
$354.00

Melanoma Marker (HMB45): m-IgG1 BP-HRP 套装

sc-532301
200 µg Ab; 20 µg BP
$354.00

Melanoma Marker 抗体 (HMB45) AC

sc-59305 AC
500 µg/ml, 25% agarose
$416.00

Melanoma Marker 抗体 (HMB45) HRP

sc-59305 HRP
200 µg/ml
$316.00

Melanoma Marker 抗体 (HMB45) FITC

sc-59305 FITC
200 µg/ml
$330.00

Melanoma Marker 抗体 (HMB45) PE

sc-59305 PE
200 µg/ml
$343.00

Melanoma Marker 抗体 (HMB45) Alexa Fluor® 488

sc-59305 AF488
200 µg/ml
$357.00

Melanoma Marker 抗体 (HMB45) Alexa Fluor® 546

sc-59305 AF546
200 µg/ml
$357.00

Melanoma Marker 抗体 (HMB45) Alexa Fluor® 594

sc-59305 AF594
200 µg/ml
$357.00

Melanoma Marker 抗体 (HMB45) Alexa Fluor® 647

sc-59305 AF647
200 µg/ml
$357.00

Melanoma Marker 抗体 (HMB45) Alexa Fluor® 680

sc-59305 AF680
200 µg/ml
$357.00

Melanoma Marker 抗体 (HMB45) Alexa Fluor® 790

sc-59305 AF790
200 µg/ml
$357.00

Would be possible to order sc-59305 with no gelatin? It would interfere with my assay

Asked by: Carlo Marchetti
Thank you for your question. Yes, this antibody is available without gelatin (in PBS + sodium azide only) as a special order. Please contact our Technical Service Department for more information and to request a quote. Please call us at 800-457-3801 option 2, or email <specialorders@scbt.com>.
Answered by: Technical Support
Date published: 2020-04-15

Hello, I have seen a publication where they used HMB-45 -APC antibody to be used on Flow-Cyto from Santa Cruz against human tumor tissue but I can't find it. Could you please point out if you have that conjugate? Best, Cristina

Asked by: Cristina C
Thank you for your question. Melanoma Marker Antibody (HMB45): sc-59305 is currently available conjugated to a variety of fluorescent dyes for use in flow cytometry, however APC is not a standard conjugate. Please contact our Special Orders Department to inquire about availability. Please call our Technical Service Department, or email <specialorders@scbt.com>.
Answered by: Technical Support
Date published: 2020-02-26

what is molecular weight of HMB 45 protein which you detected by WB?

Asked by: alexeyt
Thank you for your question. We have observed a signal at ~100KDa in Western blot tests.
Answered by: Tech Service
Date published: 2019-03-01

Can this mouse monoclonal Melanoma Marker Antibody (HMB45): sc-59305, be used for staining of mouse tissue?

Asked by: jerojero
Yes, this antibody is recommended for detection in mouse. The best way to stain mouse tissue with a mouse monoclonal, is to use a directly conjugated primary. This antibody is available conjugated to HRP (sc-59305 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-59305 PE), fluorescein (sc-59305 FITC), Alexa Fluor® 488 (sc-59305 AF488) or Alexa Fluor® 647 (sc-59305 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Support 10
Date published: 2021-04-06

How much is in each vial; I only see a concentration, not a volume

Asked by: Lisa
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Rated 4 out of 5 by from Buon funzionamento su melanoma amelanotico di caneAbbiamo testato l'anticorpo su melanoma orale di cane, con buona reattività nei melanociti tumorali.
Date published: 2024-03-19
Rated 5 out of 5 by from Strong Staining of MelanomaWe received this as a sample. We got this to work on the first try! HIER using Tris buffer pH9.0 for 30 minutes and a concentration of 1:200 looks great! We ran human (melanoma) and normal mouse skin. Both look great although we only need it for human staining.
Date published: 2017-12-20
Rated 5 out of 5 by from good resultsBand clear obtained using this antibody at 1/1000 dilution
Date published: 2017-09-08
Rated 4 out of 5 by from FonctionnelJ'ai essayé cet anticorps en culture 2 D et ça marche bien
Date published: 2017-07-25
Rated 5 out of 5 by from Effect satisfactionI highly recommend use 5% BSA diluted it at 1:500 for WB, bands were clear
Date published: 2017-06-01
Rated 5 out of 5 by from pretty resutltWe get pretty results in WB assay, with a specific band. Our samples are mouse cell , the working dilution is 1: 300.
Date published: 2017-05-27
Rated 5 out of 5 by from very happyMelanoma Marker antibody for WB detection, gives clean band
Date published: 2017-05-20
Rated 5 out of 5 by from Strong band for WBMelanoma Marker (HMB45) targets the “gp100” protein. Great for Western blot (WB).
Date published: 2016-12-28
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Melanoma Marker 抗体 (HMB45) is rated 4.8 out of 5 by 12.
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