MEK-3抗体 (5F7) 推荐用于 mouse, rat, human 和 canine 来源的MEK-3 WB, IP 和 IF检测

MEK-3 抗体 (5F7): sc-81474

MEK-3抗体 (5F7) is rated 4.0 out of 5 by 2.
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说明书

    • MEK-3 抗体 5F7 是小鼠单克隆 IgG1,提供 50 µg/0.5 ml
    • raised against N-terminus of MEK-3 of human origin
    • 推荐用于 mouse, rat, human 和 canine 来源的MEK-3 WB, IP 和 IF检测
    • 目前,我们还没有完成MEK-3 抗体 (5F7)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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I am using MEK-3 (5F7): sc-81474 for immunofluorescence, what dilution of this antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Works well for endogenous detection of MEK Works well for endogenous detection of MEK-3; used Jurkat and NIH/3T3 cell lysates. -SCBT QC
Date published: 2015-05-11
Rated 4 out of 5 by from Strong bands present in WB analysis using Strong bands present in WB analysis using transfected lysates. -SCBT QC
Date published: 2014-11-25
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