MEK-1/2抗体 (9G3) 推荐用于 mouse, rat, human 和 canine 来源的the activation loop of MEK-1 and MEK-2 (independent of its phosphorylation status) WB 和 IP检测

MEK-1/2 抗体 (9G3): sc-81504

MEK-1/2抗体 (9G3) is rated 4.5 out of 5 by 8.
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说明书

    • 抗-MEK-1/2 抗体 9G3 是小鼠单克隆 IgG2aMEK-1/2抗体, 在35篇文献中引用,规格为50 µg/0.5 ml
    • raised against phosphopeptide corresponding to the activation loop of MEK-1/2 of human origin
    • 推荐用于 mouse, rat, human 和 canine 来源的the activation loop of MEK-1 and MEK-2 (independent of its phosphorylation status) WB 和 IP检测
    • 看 MEK-1 (H-8): sc-6250,可查看有标记的 MEK-1/2 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • 关于如何获取MEK-1/2 (9G3): sc-81504免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP 是 MEK-1/2 Antibody (9G3) for WB首选应用二抗。这些试剂现在以套装形式提供MEK-1/2 Antibody (9G3) (见下方订购信息).
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I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-02-17
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Rated 4 out of 5 by from Not very clear blot Detected the bands but the film was quite unclear. Incubated the antibody in 3% v/w milk.
Date published: 2021-03-17
Rated 2 out of 5 by from please use this antibody with caution! I have previously recommended this antibody. I have thought this antibody can equally recognise the phosphor- and non-phosphor-form of MEK-1/2, according to the statement "recommended for detection of the activation loop of MEK-1 and MEK-2 (independent of its phosphorylation status)". However, recently I did a growth factor stimulation experiment. I suddenly realised that MEK-1/2 Antibody (9G3) appears to have a strong preference for the p-MEK-1/2 over non-p-MEK-1/2, but is not totally clean with non-p-MEK-1/2. Thus, it would be problematic to use this antibody, especially when the phosphorylation level is low. So, please use this antibody with caution!
Date published: 2017-10-26
Rated 5 out of 5 by from great for WB This ab has very good specificity and sensitivity for WB with MEK-1/2 from human, mouse and rat. The epitope is in the activation loop, for sequential WB using a phosphor-MEK-1/2 and this Ab, whether the binding of one ab will interfere with the binding of the other is not unknown.
Date published: 2017-01-30
Rated 5 out of 5 by from Excellent! This antibody works great in western blot analysis of MEK-1/2 expression in serum starved HeLa, Hep G2, HEK293, SH-SY5Y, MDCK, PC-12, CMT 93, Neuro 2A whole cell lysates.
Date published: 2017-01-20
Rated 5 out of 5 by from Published data for IP and WB in MCF7 breast Published data for IP and WB in MCF7 breast cancer cells. -SCBT Publication Review
Date published: 2015-05-15
Rated 5 out of 5 by from Published data for WB in rat (VSMCs) vascular Published data for WB in rat (VSMCs) vascular smooth muscle cells. -SCBT Publication Review
Date published: 2015-04-25
Rated 5 out of 5 by from Published data for IP in HEK Published data for IP in HEK-293 cells. -SCBT Publication Review
Date published: 2015-03-13
Rated 5 out of 5 by from Good Good, clear band visualized in WB in serum starved HeLa, Hep G2, HEK293, SH-SY5Y, MDCK, PC-12, CMT 93, Neuro 2A whole cell lysates. -SCBT QC
Date published: 2014-03-29
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