Date published: 2025-9-7
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megalin/LRP2 Antibody (G-9) - Immunohistochemistry - Image 369242
megalin 抗体 (G-9): sc-515750. 对福尔马林固定, 石蜡包埋的小鼠肾组织进行免疫过氧化物酶染色, 显示肾小管细胞顶端膜染色. 用 m-IgGκ BPHRP (小鼠 IgGκ 结合蛋白-HRP) 检测: sc-516102.
megalin 抗体 (G-9): sc-515750. 对福尔马林固定, 石蜡包埋的小鼠肾组织进行免疫过氧化物酶染色, 显示肾小管细胞顶端膜染色. 用 m-IgGκ BPHRP (小鼠 IgGκ 结合蛋白-HRP) 检测: sc-516102.

megalin/LRP2 抗体 (G-9): sc-515750

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说明书
  • megalin/LRP2 抗体 G-9 是小鼠单克隆 IgG1 κ，megalin/LRP2抗体, 在13篇文献中引用，规格为200 µg/ml
  • specific for an epitope mapping between amino acids 4581-4604 within a C-terminal cytoplasmic domain of megalin of rat origin
  • megalin/LRP2 抗体 (G-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse 和rat 来源的 megalin
  • 抗megalin/LRP2抗体（G-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取megalin/LRP2 (G-9): sc-515750的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 megalin/LRP2 抗体 (G-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与megalin/LRP2 抗体 (G-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Megalin抗体（G-9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠和大鼠来源的巨蛋白。Megalin (G-9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Megalin，也称为GP330，是低密度脂蛋白受体基因家族的一员，其独特结构包括富含半胱氨酸的A类重复序列簇、表皮生长因子样重复序列、YWTD重复序列和O型糖链结构域。这种复杂的结构介导着各种配体的内吞作用，包括载脂蛋白J（也称为Clusterin），后者在清除与阿尔茨海默病相关的β-淀粉样肽方面发挥着重要作用。Megalin主要位于上皮细胞的顶膜上，特别是在肾脏近端小管、肠道和脑膜细胞中，在这些地方，Megalin对于维生素B12等重要分子的重吸收至关重要。受体相关蛋白（RAP）是一种分子伴侣，通过阻止所有已知配体的吸收，促进巨蛋白的正常折叠和运输，确保细胞过程的有效功能。巨蛋白在大鼠海曼肾炎中充当抗原决定因子，凸显了巨蛋白在肾脏生理和病理中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

megalin/LRP2 抗体 (G-9) 参考文献:

  1. 在缺乏 VLDL 受体和 ApoE 受体 2 的基因敲除小鼠中，Reeler/Disabled 类神经元迁移中断。  |  Trommsdorff, M., et al. 1999. Cell. 97: 689-701. PMID: 10380922
  2. 极低密度脂蛋白受体的功能域：配体结合和酸依赖性配体解离机制的分子分析。  |  Mikhailenko, I., et al. 1999. J Cell Sci. 112 (Pt 19): 3269-81. PMID: 10504332
  3. 低密度脂蛋白受体基因家族的结构, 生化和信号特性。  |  Hussain, MM. 2001. Front Biosci. 6: D417-28. PMID: 11229872
  4. RAP 在脂蛋白受体生物生成过程中的作用。  |  Bu, G. and Marzolo, MP. 2000. Trends Cardiovasc Med. 10: 148-55. PMID: 11239794
  5. Megalin和cubilin：肾近曲小管中协同作用的内细胞受体。  |  Christensen, EI. and Birn, H. 2001. Am J Physiol Renal Physiol. 280: F562-73. PMID: 11249847
  6. 膜相关鸟苷酸激酶蛋白 MAGI-1 与巨球蛋白结合，存在于肾小球荚膜细胞中。  |  Patrie, KM., et al. 2001. J Am Soc Nephrol. 12: 667-677. PMID: 11274227
  7. 尿megalin与糖尿病肾病进展因素相关。  |  Akour, A., et al. 2019. Bratisl Lek Listy. 120: 532-535. PMID: 31602990
  8. 体内实时成像显示，巨蛋白是氨基糖苷类药物庆大霉素进入耳蜗的转运蛋白，抑制耳蜗中的庆大霉素可保护耳蜗。  |  Kim, J. and Ricci, AJ. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35197290
  9. 开发夹心酶联免疫吸附测定法，用于定量小鼠尿megalin，这是一种新型近端肾小管生物标志物。  |  Sofuku, R., et al. 2024. J Immunol Methods. 534: 113763. PMID: 39374646
  10. 确定糖蛋白 330 是脂蛋白 J/clusterin 的内细胞受体。  |  Kounnas, MZ., et al. 1995. J Biol Chem. 270: 13070-5. PMID: 7768901
  11. 脂蛋白 J-淀粉样 beta 肽复合物与低密度脂蛋白受体相关蛋白-2/megalin 的相互作用。防止淀粉样 beta 肽病理累积的机制。  |  Hammad, SM., et al. 1997. J Biol Chem. 272: 18644-9. PMID: 9228033
  12. 人类低密度脂蛋白（LDL）受体家族的受体。大型家族成员通过与复杂配体的相互作用发挥多种功能。  |  Gliemann, J. 1998. Biol Chem. 379: 951-64. PMID: 9792428

订购信息

产品名称产品编号规格价格数量收藏夹

megalin/LRP2 抗体 (G-9)

sc-515750
200 µg/ml
$316.00

megalin/LRP2 (G-9): m-IgG1 BP-HRP 套装

sc-531811
200 µg Ab; 20 µg BP
$354.00

megalin/LRP2 抗体 (G-9) AC

sc-515750 AC
500 µg/ml, 25% agarose
$416.00

megalin/LRP2 抗体 (G-9) HRP

sc-515750 HRP
200 µg/ml
$316.00

megalin/LRP2 抗体 (G-9) FITC

sc-515750 FITC
200 µg/ml
$330.00

megalin/LRP2 抗体 (G-9) PE

sc-515750 PE
200 µg/ml
$343.00

megalin/LRP2 抗体 (G-9) Alexa Fluor® 488

sc-515750 AF488
200 µg/ml
$357.00

megalin/LRP2 抗体 (G-9) Alexa Fluor® 546

sc-515750 AF546
200 µg/ml
$357.00

megalin/LRP2 抗体 (G-9) Alexa Fluor® 594

sc-515750 AF594
200 µg/ml
$357.00

megalin/LRP2 抗体 (G-9) Alexa Fluor® 647

sc-515750 AF647
200 µg/ml
$357.00

megalin/LRP2 抗体 (G-9) Alexa Fluor® 680

sc-515750 AF680
200 µg/ml
$357.00

megalin/LRP2 抗体 (G-9) Alexa Fluor® 790

sc-515750 AF790
200 µg/ml
$357.00

Hi, I have some questions in regards to this reply "We used a 3-8% gradient gel to obtain the Western blot data image shown for our megalin Antibody (G-9): sc-515750." What kind of stacking gel was used? And what were the transfer conditions? Thanks

Asked by: Mims
Thank you for your question. We use a 4% stacking gel. The conditions for the transfer depends on the electroblotting apparatus used, so please consult your manufacturer´s protocols. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2023-12-19

How can I get a sample of megalin Antibody (G-9): sc-515750 ?

Asked by: Sayed
Thank you for your question. We are currently offering a free 10 µg sample of this megalin Antibody (G-9): sc-515750. You may contact us directly, or our authorized distributor in Egypt to request a sample. For direct support, please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat. Alternatively, you can find the contact information for our distributor using the link below. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-08-22

What kind of gel was the sample image representing? Any differences between megalin Antibody (G-9) and megalin Antibody (H-10)? Have these mAbs been tested with mouse samples, in particular for mouse brains (WB and/or IHC)?

Asked by: Mtrf
Thank you for your question. We used a 3-8% gradient gel to obtain the Western blot data image shown for our megalin Antibody (G-9): sc-515750. We do offer two different clones for megalin, (G-9) and (H-10). Both antibodies are raised against the same immunogen corresponding to amino acids 4581-4604 of megalin of rat origin. At this time, the (H-10) clone has been confirmed to be reactive with mouse, rat and human samples. And the (G-9) clone has been confirmed to be reactive with mouse and rat samples only. Both antibodies have published IHC(P) data in mouse kidney tissues.
Answered by: Technical Support
Date published: 2017-03-21

Can megalin (G-9): sc-515750 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, megalin (G-9): sc-515750 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Good for Western Blot of megalinThis antibody works consistently in rat kidney tissue extract. -SCBT QC
Date published: 2023-09-14
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megalin/LRP2 抗体 (G-9) is rated 5.0 out of 5 by 1.
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