Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
MDMX Antibody (G-10) - Western Blotting - Image 17025
MDMX 抗体 (G-10): sc-74467. 对 Sol8 全细胞裂解液 (A) 和 Hep G2 核提取物 (B) 中 MDMX 的表达进行 Western 印迹分析.
MDMX 抗体 (G-10): sc-74467. 对 Sol8 全细胞裂解液 (A) 和 Hep G2 核提取物 (B) 中 MDMX 的表达进行 Western 印迹分析.

MDMX 抗体 (G-10): sc-74467

3.7(6)
寫評論提問

说明书
  • MDMX 抗体 G-10 是小鼠单克隆 IgG2a κ，MDMX抗体, 在3篇文献中引用，规格为200 µg/ml
  • 针对氨基酸361-490，在MDMX的human起源的C-terminus处进行映射
  • MDMX 抗体 (G-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 MDMX
  • 抗MDMX抗体（G-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MDMX (G-10): sc-74467的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是MDMX Antibody (G-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MDMX Antibody (G-10) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

MDMX抗体（G-10）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的MDMX蛋白。抗MDMX抗体（G-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。MDMX蛋白在调节p53蛋白稳定性方面发挥着关键作用，而p53蛋白对于维持细胞完整性和防止肿瘤形成至关重要。MDMX是一种与MDM2相关的蛋白，通过不同的相互作用抑制由MDM2介导的p53降解，从而影响p53在细胞周期调节和细胞凋亡中的作用。MDMX（也称为MDM4）在恶性胶质瘤中经常被放大，这凸显了其在癌症生物学中的重要性。此外，MDMX与ARF蛋白相互作用，后者将MDMX隔离在核仁中，从而增强p53的转激活作用并提高p53的活性。MDMX、MDM2和p53之间的这种动态相互作用凸显了MDMX在调节关键细胞过程方面的重要性，以及其在癌症治疗中作为治疗靶点的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MDMX 抗体 (G-10) 参考文献:

  1. 靶向p53-MDM2-MDMX环路的癌症治疗。  |  Zhang, Q., et al. 2014. Subcell Biochem. 85: 281-319. PMID: 25201201
  2. MDM2和MDMX在癌症中的作用。  |  Karni-Schmidt, O., et al. 2016. Annu Rev Pathol. 11: 617-44. PMID: 27022975
  3. MDM2/MDMX-p53信号轴的小鼠模型。  |  Tackmann, NR. and Zhang, Y. 2017. J Mol Cell Biol. 9: 34-44. PMID: 28096294
  4. MDM2 和 MDMX 通过 PPARα 介导的脂质重塑促进铁变态反应。  |  Venkatesh, D., et al. 2020. Genes Dev. 34: 526-543. PMID: 32079652
  5. MDMX是一种普遍存在的白血病前期到急性髓细胞白血病过渡机制。  |  Ueda, K., et al. 2021. Cancer Cell. 39: 529-547.e7. PMID: 33667384
  6. MDMX驱动白血病前期进展。  |  Seton-Rogers, S. 2021. Nat Rev Cancer. 21: 280. PMID: 33762752
  7. miRNA在MDMX-p53相互作用中的作用。  |  Yu, D., et al. 2021. J Evid Based Med. 14: 152-160. PMID: 33988919
  8. 癌症治疗中的治疗机会：靶向p53-MDM2/MDMX相互作用。  |  Munisamy, M., et al. 2021. Am J Cancer Res. 11: 5762-5781. PMID: 35018225
  9. 适应性应激反应中的MDM2/MDMX/p53轴。  |  Wang, B., et al. 2020. Transl Cancer Res. 9: 1993-1997. PMID: 35117546
  10. 维尔贝纳林通过调节 MDMX/PPARα 介导的铁蛋白沉积，减轻酒精相关性脂肪性肝炎的肝损伤和线粒体功能障碍。  |  Dong, J., et al. 2023. J Ethnopharmacol. 307: 116227. PMID: 36739928

订购信息

产品名称产品编号规格价格数量收藏夹

MDMX 抗体 (G-10)

sc-74467
200 µg/ml
$316.00

MDMX (G-10): m-IgG Fc BP-HRP 套装

sc-525716
200 µg Ab; 10 µg BP
$354.00

MDMX (G-10): m-IgG2a BP-HRP 套装

sc-546376
200 µg Ab; 10 µg BP
$354.00

MDMX 抗体 (G-10) AC

sc-74467 AC
500 µg/ml, 25% agarose
$416.00

MDMX 抗体 (G-10) HRP

sc-74467 HRP
200 µg/ml
$316.00

MDMX 抗体 (G-10) FITC

sc-74467 FITC
200 µg/ml
$330.00

MDMX 抗体 (G-10) PE

sc-74467 PE
200 µg/ml
$343.00

MDMX 抗体 (G-10) Alexa Fluor® 488

sc-74467 AF488
200 µg/ml
$357.00

MDMX 抗体 (G-10) Alexa Fluor® 546

sc-74467 AF546
200 µg/ml
$357.00

MDMX 抗体 (G-10) Alexa Fluor® 594

sc-74467 AF594
200 µg/ml
$357.00

MDMX 抗体 (G-10) Alexa Fluor® 647

sc-74467 AF647
200 µg/ml
$357.00

MDMX 抗体 (G-10) Alexa Fluor® 680

sc-74467 AF680
200 µg/ml
$357.00

MDMX 抗体 (G-10) Alexa Fluor® 790

sc-74467 AF790
200 µg/ml
$357.00

I am using MDMX mouse monoclonal antibody (D-4) (SC-74468) on protein from MNCs for western blotting. I am not getting the 80kDa band but a band at ~43 kDa in every sample. Just wondered if this a nonpecific or a some form of cleaved MDM4. Suggestions?

Asked by: BKM8
Thank you for your question. We have not tested this antibody in mononuclear cells (MNCs), and have not observed a signal ~43 kDa by Western blotting in the cell lines tested. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-26

Can MDMX (G-10): sc-74467 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MDMX (G-10): sc-74467 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_5, h_6CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_74467, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 116ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from MDMXThis is the second MDMX antibody I used from SC and I see bands at 37kDa which is unexpected of.
Date published: 2018-04-12
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from Good for WB1:500 diluted with BSA,for the WB of rat protein,the band is special ,but not clear!
Date published: 2017-06-06
Rated 5 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of MDMX expression in Sol8 whole cell lysate and Hep G2 nuclear extract. -SCBT QC
Date published: 2015-06-04
Rated 5 out of 5 by from Clean bands in western blot using MCF7Clean bands in western blot using MCF7, U2OS, and SK-HEP-1 whole cell lysates. -SCBT Publication Review
Date published: 2015-05-03
Rated 5 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-04-08
  • y_2025, m_9, d_5, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_74467, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 19ms
  • REVIEWS, PRODUCT
MDMX抗体 (G-10) is rated 3.7 out of 5 by 6.
  • y_2025, m_9, d_5, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_zh_CN, sid_74467, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 111ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。