Date published: 2025-9-7
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MCT4 Antibody (F-10) - Western Blotting - Image 376206
MCT4 抗体 (F-10): sc-376101. SJRH30 (A), BC3H1 (B) 和 Neuro-2A (C) 全细胞裂解液中 MCT4 表达的 Western 印迹分析.
MCT4 抗体 (F-10): sc-376101. SJRH30 (A), BC3H1 (B) 和 Neuro-2A (C) 全细胞裂解液中 MCT4 表达的 Western 印迹分析.

MCT4 抗体 (F-10): sc-376101

3.8(5)
寫評論提問

说明书
  • MCT4抗体(F-10)是小鼠单克隆IgG1 κ, 在10篇文献中引用，规格为200 µg/ml
  • 免疫human来源MCT4位于C-terminal细胞质域的376-465氨基酸
  • 抗-MCT4 抗体 (F-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MCT4
  • 抗MCT4抗体（F-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MCT4 (F-10): sc-376101的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是MCT4 Antibody (F-10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与MCT4 Antibody (F-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外MCT抗体，包括MCT1, MCT11, MCT13, MCT2和MCT4

MCT4抗体（F-10）是一种IgG1 κ小鼠单克隆MCT4抗体（也被称为MCT4抗体或SLC16A3抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类来源的MCT4。MCT4抗体（F-10）既有非偶联形式的抗MCT4抗体，也有多种偶联形式的抗MCT4抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。单羧酸盐，如乳酸和丙酮酸，在细胞代谢中起着不可或缺的作用。乳酸是糖酵解过程中大量产生的，在正常生理条件下为细胞提供大部分ATP。然而，乳酸的积累会导致细胞内pH值下降和糖酵解停止。为了使糖酵解以高速率继续进行，必须将乳酸运出细胞。这一转运过程由单羧酸盐转运体（MCTs）家族完成，它们作为质子同向转运体，对L-乳酸具有立体选择性。MCT家族至少包括八个成员，即MCT1-8，它们含有10-12个跨膜螺旋（TM）结构域，氨基和羧基末端位于细胞质中。MCT1广泛表达，是肿瘤细胞和红细胞中MCT的主要形式。MCT2在肝脏和睾丸中高表达，而MCT3和MCT4主要在骨骼肌中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MCT4 抗体 (F-10)

sc-376101
200 µg/ml
$316.00

MCT4 (F-10): m-IgG Fc BP-HRP 套装

sc-529821
200 µg Ab; 10 µg BP
$354.00

MCT4 (F-10): m-IgGκ BP-HRP 套装

sc-522844
200 µg Ab, 40 µg BP
$354.00

MCT4 (F-10): m-IgG1 BP-HRP 套装

sc-543707
200 µg Ab; 20 µg BP
$354.00

MCT4 抗体 (F-10) AC

sc-376101 AC
500 µg/ml, 25% agarose
$416.00

MCT4 抗体 (F-10) HRP

sc-376101 HRP
200 µg/ml
$316.00

MCT4 抗体 (F-10) FITC

sc-376101 FITC
200 µg/ml
$330.00

MCT4 抗体 (F-10) PE

sc-376101 PE
200 µg/ml
$343.00

MCT4 抗体 (F-10) Alexa Fluor® 488

sc-376101 AF488
200 µg/ml
$357.00

MCT4 抗体 (F-10) Alexa Fluor® 546

sc-376101 AF546
200 µg/ml
$357.00

MCT4 抗体 (F-10) Alexa Fluor® 594

sc-376101 AF594
200 µg/ml
$357.00

MCT4 抗体 (F-10) Alexa Fluor® 647

sc-376101 AF647
200 µg/ml
$357.00

MCT4 抗体 (F-10) Alexa Fluor® 680

sc-376101 AF680
200 µg/ml
$357.00

MCT4 抗体 (F-10) Alexa Fluor® 790

sc-376101 AF790
200 µg/ml
$357.00

I am using MCT4 (F-10): sc-376101 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Trav11
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from not good for my mouse sample.only show weak band at proper molecular site, which sample have show a good band using antibody from other source.
Date published: 2017-11-02
Rated 5 out of 5 by from Versatile Monoclonal AntibodyExcellent monoclonal antibody for multiple applications. Observed strong signal by WB, IHC (P) and IF in multiple samples
Date published: 2017-01-21
Rated 4 out of 5 by from Good signal with HeLa whole cell lysate in WBGood signal with HeLa whole cell lysate in WB. -SCBT QC
Date published: 2015-04-21
Rated 4 out of 5 by from Worked well for us at a dilution of 1:200Worked well for us at a dilution of 1:200 with a secondary anti-mouse HRP at 1:5000. It was on Panc1 cell lysates and we loaded 15 ug per sample. It was a little smeary at higher molecular weight but it was cleaner at lower molecular weights until the band which was pretty well defined.
Date published: 2015-03-26
Rated 5 out of 5 by from Great immunoperoxidase membrane and cytoplasmicGreat immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-02-19
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MCT4 抗体 (F-10) is rated 3.8 out of 5 by 5.
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