Date published: 2025-9-5
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MCT4 Antibody (D-1) - Western Blotting - Image 372688
MCT4 抗体 (D-1): sc-376140. HeLa (A), HCT-116 (B), NCI-H460 (C) 和 PC-3 (D) 全细胞裂解液中 MCT4 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
MCT4 抗体 (D-1): sc-376140. HeLa (A), HCT-116 (B), NCI-H460 (C) 和 PC-3 (D) 全细胞裂解液中 MCT4 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

MCT4 抗体 (D-1): sc-376140

4.1(28)
寫評論提問

说明书
  • MCT4 抗体 D-1 是小鼠单克隆 IgG2a κ，MCT4抗体, 在83篇文献中引用，规格为200 µg/ml
  • 免疫human来源MCT4位于C-terminal细胞质域的376-465氨基酸
  • MCT4 抗体 (D-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MCT4
  • 抗MCT4抗体（D-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MCT4 (D-1): sc-376140的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是MCT4 Antibody (D-1) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与MCT4 Antibody (D-1) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

额外MCT抗体，包括MCT1, MCT11, MCT13, MCT2和MCT4

MCT4抗体（D-1）是一种IgG2a κ小鼠单克隆MCT4抗体（也被称为SLC16A3抗体、溶质载体家族16成员3抗体或MCT3抗体），可通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人类来源的MCT4蛋白。MCT4抗体（D-1）既有非偶联形式的抗MCT4抗体，也有多种偶联形式的抗MCT4抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。单羧酸盐，如乳酸和丙酮酸，在细胞代谢中起着不可或缺的作用。乳酸是糖酵解过程中大量产生的，在正常生理条件下为细胞提供大部分ATP。然而，乳酸的积累会导致细胞内pH值下降和糖酵解停止。为了使糖酵解以高速率继续进行，必须将乳酸运出细胞。这一转运过程由单羧酸盐转运体（MCTs）家族完成，它们作为质子同向转运体，对L-乳酸具有立体选择性。MCT家族至少包括八个成员，即MCT1-8，它们含有10-12个跨膜螺旋（TM）结构域，氨基和羧基末端位于细胞质中。MCT1广泛表达，是肿瘤细胞和红细胞中MCT的主要形式。MCT2在肝脏和睾丸中高表达，而MCT3和MCT4主要在骨骼肌中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MCT4 抗体 (D-1) 参考文献:

  1. 骨骼肌中的乳酸盐转运--单羧酸盐转运体的作用和调节。  |  Juel, C. and Halestrap, AP. 1999. J Physiol. 517 (Pt 3): 633-42. PMID: 10358105
  2. 质子连接单羧酸盐转运体（MCT）家族：结构, 功能和调节。  |  Halestrap, AP. and Price, NT. 1999. Biochem J. 343 Pt 2: 281-99. PMID: 10510291
  3. 心脏和骨骼肌中的乳酸转运体（MCT 蛋白）。  |  Bonen, A. 2000. Med Sci Sports Exerc. 32: 778-89. PMID: 10776897
  4. 心脏和快速运动骨骼肌中 MCT1 和 MCT4 的丰度和亚细胞分布。  |  Bonen, A., et al. 2000. Am J Physiol Endocrinol Metab. 278: E1067-77. PMID: 10827010
  5. 成年大鼠和哺乳大鼠脑内皮细胞和神经胶质对单羧酸盐转运体 MCT1 的表达。  |  Gerhart, DZ., et al. 1997. Am J Physiol. 273: E207-13. PMID: 9252498
  6. 与心肌缺血有关的心脏乳酸转运  |  Halestrap, AP., et al. 1997. Am J Cardiol. 80: 17A-25A. PMID: 9293952
  7. 四种新的哺乳动物单羧酸盐转运体（MCT）同源物的克隆和测序证实了一个古老的转运体家族的存在。  |  Price, NT., et al. 1998. Biochem J. 329 (Pt 2): 321-8. PMID: 9425115
  8. 人类单羧酸盐转运体 2（MCT2）是一种高亲和性丙酮酸盐转运体。  |  Lin, RY., et al. 1998. J Biol Chem. 273: 28959-65. PMID: 9786900

订购信息

产品名称产品编号规格价格数量收藏夹

MCT4 抗体 (D-1)

sc-376140
200 µg/ml
$316.00

MCT4 (D-1): m-IgGκ BP-HRP 套装

sc-522851
200 µg Ab, 40 µg BP
$354.00

MCT4 抗体 (D-1) AC

sc-376140 AC
500 µg/ml, 25% agarose
$416.00

MCT4 抗体 (D-1) HRP

sc-376140 HRP
200 µg/ml
$316.00

MCT4 抗体 (D-1) FITC

sc-376140 FITC
200 µg/ml
$330.00

MCT4 抗体 (D-1) PE

sc-376140 PE
200 µg/ml
$343.00

MCT4 抗体 (D-1) Alexa Fluor® 488

sc-376140 AF488
200 µg/ml
$357.00

MCT4 抗体 (D-1) Alexa Fluor® 546

sc-376140 AF546
200 µg/ml
$357.00

MCT4 抗体 (D-1) Alexa Fluor® 594

sc-376140 AF594
200 µg/ml
$357.00

MCT4 抗体 (D-1) Alexa Fluor® 647

sc-376140 AF647
200 µg/ml
$357.00

MCT4 抗体 (D-1) Alexa Fluor® 680

sc-376140 AF680
200 µg/ml
$357.00

MCT4 抗体 (D-1) Alexa Fluor® 790

sc-376140 AF790
200 µg/ml
$357.00

FCM用にsc-376140 AF488を購入したのですが、こちらについては検証されていないのでしょうか？MCT4 (D-1) is available conjugated to Alexa Fluor® 488 (sc-376140 AF488) for WB (RGB), IF, IHC(P) and FC.とあるのですが。

Asked by: Tomoya
Thank you for your product review.Please contact our Technical Service Department of Japan for the consultation optimization suggestion and more information. 商品の問題、又は質問がございましたら、日本のディーラーを連絡し、お尋ねください。宜しくお願い致します。
Answered by: Jiajun Z
Date published: 2024-11-07

I am planning to use this antibody for detecting mouse MCT4 by FCM. Can you confirm that this antibody specifically targets the cytoplasmic domain of MCT4 and is suitable for use in both mouse and human samples?

Asked by: Tomoya
Thank you for your question. The MCT4 Antibody (D-1): sc-376140 has not been tested for FCM and is not recommended for FCM at this time. 質問ありがとうございます。なお、このmct4抗体(d-1) sc-376140については、fcm用に試験しておりませんので、現時点では推奨しておりません。
Answered by: Yanru Tao
Date published: 2024-11-05

MCT-4抗体の10 μgの無料サンプルを頂きIHCをお試したいのですが、いかがでしょうか。

Asked by: RyosukeY
質問頂き、誠にありがとうございます。抗体の購入とサンプル品の申請は日本のディーラーを連絡し、お尋ねください。宜しくお願い致します。 https://www.scbt.com/ja/resources/distributors?&_requestid=1655 Thank you very much for your question. Please contact your Japanese dealer and ask them to purchase antibodies and apply for sample products. Thank you for your cooperation. https://www.scbt.com/ja/resources/distributors?&_requestid=1655
Answered by: Jiajun Z
Date published: 2024-09-20

Which specific antigen retrieval do you recommand using MCT4 (D-1): sc-376140 antibody between :10 mM sodium citrate buffer, pH 6.0 (sc-294091); 50 mM glycine-HCl buffer (sc-29096), pH 3.5, and 0.01% (w/v) EDTA (sc-29092)?

Asked by: Nina 44
Thank you for your question. For antigen retrieval in experiments with this antibody we recommend heat treatment: Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Tech Support Europe
Date published: 2022-06-22

Which specific antigen retrieval do you recommand using MCT4 (D-1): sc-376140 antibody for IHC(P)? Your protocol (immunoperoxidase-staining Antigen retrieval is described in step C) does not specify for this antibody specifically.

Asked by: Nina 44
Thank you for your question. For antigen retrieval in experiments with this antibody we recommend heat treatment: Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Tech Support Europe
Date published: 2022-06-21

Can MCT4 antibody (D-1): sc-376140 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections? Which antigen retrieval do you recommand?

Asked by: Nina 44
Thank you for your question. sc-376140 is recommended to be used in IHC(P). You can find a detailed protocol by following the link below: https://www.scbt.com/resources/protocols/immunoperoxidase-staining Antigen retrieval is described in step C.
Answered by: Tech Support Europe
Date published: 2022-06-21

Would it be possible to purchase a sample of this antibody? MCT4 Antibody (D-1): sc-376140

Asked by: LCos
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service 1
Date published: 2021-01-28

I tried on mouse sample (4T1 cells lysate ) for WB, but doesn't work. is it possible to have the an aliquot of the discontinued MCT4 (sc-50329) that was working in my previous experiments?

Asked by: AnnaTo
Thank you for your question. The rabbit polyclonal antibody, MCT4 (H-90): sc-50329, has been discontinued since 2016, and is no longer available for sale or distribution. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2022-06-21
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Rated 5 out of 5 by from 功能很好我们团队在此买过抗体，说是功能很好，现在也推荐我来买了，看看这一次功能怎么样，用过一段时间之后我再来评论。
Date published: 2025-03-03
Rated 4 out of 5 by from Works wellUsed in WB and the clear band was detected. it worked well with rat samples.
Date published: 2023-06-21
Rated 3 out of 5 by from Worked for mouse proteinThis antibody seemed to pick up only the mouse protein, as opposed to a different one I tried before that only provided signal for the human cell line. At least I know they both express it!
Date published: 2022-01-05
Rated 5 out of 5 by from GoodUsed on HeLa, PC-3 and MCF7 cells, dil 1:1000, both Western blotting and immunofluorescence. Works well
Date published: 2021-06-24
Rated 5 out of 5 by from WonderfulThis is a very stable antibody, and the price is very good
Date published: 2020-09-02
Rated 5 out of 5 by from good for IHC and wester blottingStaining of 10um lung tissue slices looked good following concentrations suggested by SCBT. For Western blotting, 1/000 dillution is enough.
Date published: 2019-06-09
Rated 1 out of 5 by from no bandsno bands being detected in human melanoma cells, even the positive control was negative
Date published: 2019-04-16
Rated 5 out of 5 by from Anticuerpo con buen resultado por WBHemos obtenido unos resultados buenos en WB usando una concentración 1/1000. Se obtiene una banda es fuerte y específica.
Date published: 2019-03-18
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MCT4 抗体 (D-1) is rated 4.1 out of 5 by 28.
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