Date published: 2025-9-8

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Mcl-1 抗体 (B-6): sc-74436

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说明书
  • Mcl-1抗体(B-6)是小鼠单克隆IgG2a κ, 在9篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Mcl-1的氨基酸1-260
  • 抗-Mcl-1 抗体 (B-6) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Mcl-1
  • 抗Mcl-1抗体(B-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mcl-1 (B-6): sc-74436免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是Mcl-1 Antibody (B-6) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Mcl-1 Antibody (B-6) 打包提供(请参阅下面的订购信息)。

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関連項目

Mcl-1抗体(B-6)是一种IgG2aκ小鼠单克隆Mcl-1抗体(也称为MCL1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Mcl-1。Mcl-1抗体(B-6)可作为非偶联的抗Mcl-1抗体形式,以及多种偶联形式的抗Mcl-1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。B细胞慢性淋巴细胞白血病/淋巴瘤2(Bcl-2)能阻止细胞在接受各种刺激后发生死亡,并在生长因子撤除后赋予某些造血细胞系以抗死亡效应。髓系细胞白血病1(Mcl-1)与Bcl-2具有序列同源性,并且与Bcl-2进一步相似,因为它的表达促进了细胞活性。p53和Mcl-1通过介导Bcl-2拮抗剂杀手(Bak)活性对线粒体凋亡产生相反的作用。Mcl-1是早期造血祖细胞稳态所必需的重要且特异的调节因子。糖原合成酶激酶3(GSK3)控制Mcl-1的稳定性,这影响生长因子、PI 3激酶和AKT对细胞凋亡的调控。Mcl-1蛋白缺乏的小鼠显示B淋巴细胞和T淋巴细胞显著减少,这与在IL-7或IL-7R缺乏的小鼠中观察到的效果相似。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Mcl-1 抗体 (B-6)

sc-74436
200 µg/ml
$316.00

Mcl-1 (B-6): m-IgG Fc BP-HRP 套装

sc-525707
200 µg Ab; 10 µg BP
$354.00

Mcl-1 (B-6): m-IgG2a BP-HRP 套装

sc-546369
200 µg Ab; 10 µg BP
$354.00

Mcl-1 抗体 (B-6) AC

sc-74436 AC
500 µg/ml, 25% agarose
$416.00

Mcl-1 抗体 (B-6) HRP

sc-74436 HRP
200 µg/ml
$316.00

Mcl-1 抗体 (B-6) FITC

sc-74436 FITC
200 µg/ml
$330.00

Mcl-1 抗体 (B-6) PE

sc-74436 PE
200 µg/ml
$343.00

Mcl-1 抗体 (B-6) Alexa Fluor® 488

sc-74436 AF488
200 µg/ml
$357.00

Mcl-1 抗体 (B-6) Alexa Fluor® 546

sc-74436 AF546
200 µg/ml
$357.00

Mcl-1 抗体 (B-6) Alexa Fluor® 594

sc-74436 AF594
200 µg/ml
$357.00

Mcl-1 抗体 (B-6) Alexa Fluor® 647

sc-74436 AF647
200 µg/ml
$357.00

Mcl-1 抗体 (B-6) Alexa Fluor® 680

sc-74436 AF680
200 µg/ml
$357.00

Mcl-1 抗体 (B-6) Alexa Fluor® 790

sc-74436 AF790
200 µg/ml
$357.00

How can Mcl-1 (B-6): sc-74436 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: TinTin
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to HRP (sc-74436 HRP), phycoerythrin (sc-74436 PE), fluoroscein (sc-74436 FITC) and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Good for WB1:500 diluted for wb of rat heart protein,there exists two bands just as the example picture in the direction
Date published: 2017-11-05
Rated 3 out of 5 by from Pas de problème d'aspécificitéAnticorps utilisé en western blot pour détecter Mcl1 dans des extraits protéiques de cellules COV434 (13 µg d'extraits). Une seule bande de faible intensité détectée après 30 minutes d'exposition. Cette bande pourrait correspondre à la forme longue de Mcl1. Il est possible que Mcl1 soit trop faiblement exprimé dans les COV434.
Date published: 2017-10-11
Rated 5 out of 5 by from Great!We received a sample antibody to try for immunofluorescence. It worked well!
Date published: 2016-12-29
Rated 5 out of 5 by from Strong band in WB in mouse transfected 293TStrong band in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by from Robust signal in WB in RamosRobust signal in WB in Ramos, K-562 and AML-193 whole cell lysates. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Good specificity in WB in BJAB whole cell lysatesGood specificity in WB in BJAB whole cell lysates. -SCBT QC
Date published: 2015-01-26
Rated 5 out of 5 by from Good publishable data in WB in human myelomaGood publishable data in WB in human myeloma cell line. -SCBT Publication Review
Date published: 2014-11-10
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Mcl-1 抗体 (B-6) is rated 4.7 out of 5 by 7.
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