Date published: 2025-9-11
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MBP-probe Antibody (R29.6) - Western Blotting - Image 5599
MBP探针抗体（R29.6）：sc-13564。MBP标签融合蛋白的Western blot分析。
MBP-probe Antibody (R29.6) - Western Blotting - Image 5599
MBP探针抗体（R29.6）：sc-13564。MBP标签融合蛋白的Western blot分析。
MBP探针抗体（R29.6）：sc-13564。MBP标签融合蛋白的Western blot分析。

MBP-probe 抗体 (R29.6): sc-13564

4.8(8)
寫評論提問

说明书
  • MBP-probe 抗体 R29.6 是小鼠单克隆 IgG1 κ，MBP-probe抗体, 在27篇文献中引用，规格为200 µg/ml
  • 针对麦芽糖结合蛋白（MBP）融合蛋白
  • MBP-probe 抗体 (R29.6) 推荐用于 WB 和 IP，检测 MBP fusion proteins
  • MBP-probe抗体（R29.6）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MBP-probe (R29.6): sc-13564的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成MBP-probe 抗体 (R29.6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

Maltodextrin Binding Protein 抗体

MBP-probe抗体（R29.6）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）检测MBP-probe蛋白。抗MBP探针抗体（R29.6）有非结合型和多种结合型，包括琼脂糖、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。MBP-探针蛋白源自大肠杆菌的malE基因，由370个氨基酸组成，在促进混合融合蛋白的纯化方面发挥着关键作用。这在分子生物学中尤为重要，因为MBP结构域允许使用淀粉酶树脂通过亲和色谱法有效分离目标蛋白。一旦MBP融合蛋白与树脂结合，就可以通过凝血因子Xa的切割或过量游离麦芽糖的洗脱与目标蛋白分离，从而使研究人员获得高纯度的蛋白质用于进一步分析。通过蛋白质印迹（WB）和免疫沉淀（IP）等技术对这些蛋白质进行可视化，可提高MBP探针（R29.6）抗体的实用性，使其成为在原核和真核系统中研究融合蛋白的必备工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MBP-probe 抗体 (R29.6) 参考文献:

  1. 纯化与麦芽糖结合蛋白融合的蛋白质。  |  Lebendiker, M. and Danieli, T. 2011. Methods Mol Biol. 681: 281-93. PMID: 20978971
  2. 麦芽糖结合蛋白是一种潜在的口服免疫载体。  |  Bellot, P., et al. 2013. Vet Immunol Immunopathol. 152: 101-8. PMID: 23078905
  3. 提高融合伙伴的溶解度是麦芽糖结合蛋白的固有特性，但它们的折叠要么是自发的，要么是由伴侣介导的。  |  Raran-Kurussi, S. and Waugh, DS. 2012. PLoS One. 7: e49589. PMID: 23166722
  4. 麦芽糖结合蛋白（MBP）--哺乳动物蛋白质表达系统的分泌增强标记。  |  Reuten, R., et al. 2016. PLoS One. 11: e0152386. PMID: 27029048
  5. 通过与麦芽糖结合蛋白融合，促进外来多肽在大肠杆菌中表达和纯化的载体。  |  di Guan, C., et al. 1988. Gene. 67: 21-30. PMID: 2843437
  6. 单步纯化在大肠杆菌中表达的与谷胱甘肽 S-转移酶融合的多肽。  |  Smith, DB. and Johnson, KS. 1988. Gene. 67: 31-40. PMID: 3047011
  7. 大肠杆菌载体，通过与麦芽糖结合蛋白融合和分离，表达和纯化外来蛋白。  |  Maina, CV., et al. 1988. Gene. 74: 365-73. PMID: 3073105
  8. 一个有用的表位标签来自麦芽糖结合蛋白。  |  Lénon, M., et al. 2021. Protein Sci. 30: 1235-1246. PMID: 33896065
  9. 肽配体与标记为降钙素基因相关肽受体的麦芽糖结合蛋白的相互作用：受体 N-糖基化的抑制作用。  |  Lee, S. 2022. Peptides. 150: 170735. PMID: 35007660
  10. 大肠杆菌 K12 的 malE 基因及其产物麦芽糖结合蛋白的序列。  |  Duplay, P., et al. 1984. J Biol Chem. 259: 10606-13. PMID: 6088507
  11. 多功能原核蛋白质表达系统：过量生产, 亲和纯化和选择性检测。  |  Kroll, DJ., et al. 1993. DNA Cell Biol. 12: 441-53. PMID: 7686011

订购信息

产品名称产品编号规格价格数量收藏夹

MBP-probe 抗体 (R29.6)

sc-13564
200 µg/ml
$316.00

MBP-probe 抗体 (R29.6) AC

sc-13564 AC
500 µg/ml, 25% agarose
$416.00

MBP-probe 抗体 (R29.6) FITC

sc-13564 FITC
200 µg/ml
$330.00

MBP-probe 抗体 (R29.6) PE

sc-13564 PE
200 µg/ml
$343.00

MBP-probe 抗体 (R29.6) Alexa Fluor® 488

sc-13564 AF488
200 µg/ml
$357.00

MBP-probe 抗体 (R29.6) Alexa Fluor® 546

sc-13564 AF546
200 µg/ml
$357.00

MBP-probe 抗体 (R29.6) Alexa Fluor® 594

sc-13564 AF594
200 µg/ml
$357.00

MBP-probe 抗体 (R29.6) Alexa Fluor® 647

sc-13564 AF647
200 µg/ml
$357.00

MBP-probe 抗体 (R29.6) Alexa Fluor® 680

sc-13564 AF680
200 µg/ml
$357.00

MBP-probe 抗体 (R29.6) Alexa Fluor® 790

sc-13564 AF790
200 µg/ml
$357.00

Is it possible to use as secondary antibody the anti-mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit (Sigma A9044) against the MBP-probe antibody (R29.6) (Santa Cruz-sc-13564)?

Asked by: Izis
Thank you for your question. For secondary detection of our antibodies using HRP we recommend our secondary binding protein m-IgGκ BP-HRP: sc-516102.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

What is the species reactivity of this antibody?

Asked by: AbPolly
Thank you for your question. This antibody is not species specific. It will detect MBP fusion proteins in any sample.
Answered by: Technical Support 7
Date published: 2017-02-07
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Rated 5 out of 5 by from funktiooniert gut im western blotschönes signal bei aufreinigung von fusionsproteinen
Date published: 2017-09-29
Rated 5 out of 5 by from Excellent detectionThis antibody gave excellent detection of an MBP-tagged protein of large size.
Date published: 2017-08-11
Rated 5 out of 5 by from Reliable antibodyMBP-probe Antibody (R29.6) is a reliable choice for detection of MBP fusion proteins and has been featured in over a dozen publications!
Date published: 2017-01-26
Rated 3 out of 5 by from Worked well with MBPWorked well with MBP-tagged fusion protein. -SCBT QC
Date published: 2015-06-14
Rated 5 out of 5 by from Successful application of this antibody inSuccessful application of this antibody in WB using HeLa S-100 and HEK293T cells -SCBT Publication Review
Date published: 2015-04-25
Rated 5 out of 5 by from Publishable ELISA and confocal microscopyPublishable ELISA and confocal microscopy data with human Beas-2B - SCBT Publication Review
Date published: 2015-02-25
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for WB on monkey and mouse Cos-7, N1E-115 cells, and 293T cells - SCBT Publication Review
Date published: 2015-02-16
Rated 5 out of 5 by from WB publishable data with eWB publishable data with e. coli recombinant proteins - SCBT Publication Review
Date published: 2013-12-28
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MBP-probe 抗体 (R29.6) is rated 4.8 out of 5 by 8.
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