Date published: 2025-9-8
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MAN2A1 Antibody (D-5) - Western Blotting - Image 375394
MAN2A1 抗体 (D-5): sc-377204. 在 JAR (A), NCI-H929 (B) 和 U-87 MG (C) 全细胞裂解液中 MAN2A1 表达的 Western 印迹分析.
MAN2A1 抗体 (D-5): sc-377204. 在 JAR (A), NCI-H929 (B) 和 U-87 MG (C) 全细胞裂解液中 MAN2A1 表达的 Western 印迹分析.

MAN2A1 抗体 (D-5): sc-377204

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说明书
  • MAN2A1 抗体 D-5 是小鼠单克隆 IgG1 κ，MAN2A1抗体, 在2篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列584-805位于human物种的MAN2A1的内部区域内
  • MAN2A1 抗体 (D-5) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 MAN2A1
  • 抗MAN2A1抗体（D-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MAN2A1 (D-5): sc-377204的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是MAN2A1 Antibody (D-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与MAN2A1 Antibody (D-5) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外mannosidase抗体，包括MAN1B1, MAN2A1和MAN2C1

MAN2A1抗体（D-5）是一种IgG1 κ型小鼠单克隆MAN2A1抗体（也称为MAN2A1抗体），可以通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附试验等方法检测人源MAN2A1蛋白。MAN2A1抗体（D-5）可以以非共轭抗MAN2A1抗体形式提供，也可以以多种共轭形式的抗MAN2A1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。α-甘露糖苷酶（包括MAN1A1、MAN1A2、MAN2A1和MAN2A2）是一组可溶性蛋白质，它们定位于内质网、高尔基体或细胞质中。这些蛋白质根据其细胞定位参与新合成的N-聚糖的加工或降解。MAN2A1（甘露糖苷酶α类2A成员1）是一种单次跨膜II型膜蛋白，定位于高尔基体的膜囊，并参与蛋白质修饰途径。更具体地说，MAN2A1使用锌作为辅因子来催化N-聚糖形成过程中的第一个关键步骤，即寡糖Man5(GlcNAc)3中末端α-D-甘露糖残基的水解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MAN2A1 抗体 (D-5) 参考文献:

  1. 人类胚胎肾293T细胞系在高尔基α-甘露糖苷酶II基因座发生突变。  |  Crispin, M., et al. 2009. J Biol Chem. 284: 21684-95. PMID: 19465480
  2. HCV29膀胱上皮细胞上皮-间质转化过程中N-糖链及其相关糖原基因的表达变化。  |  Guo, J., et al. 2014. Molecules. 19: 20073-90. PMID: 25470275
  3. 通过Cas9介导的自杀基因插入靶向肿瘤细胞中的基因组重排。  |  Chen, ZH., et al. 2017. Nat Biotechnol. 35: 543-550. PMID: 28459452
  4. 甘露糖糖基化是NIS定位和功能在人类乳腺癌细胞中不可或缺的步骤。  |  Rathod, M., et al. 2019. J Cell Sci. 132: PMID: 31455607
  5. 对与败血症相关的基因座的功能注释优先考虑免疫和内皮细胞通路。  |  Le, KTT., et al. 2019. Front Immunol. 10: 1949. PMID: 31475010
  6. 精神分裂症患者精神分裂症相关基因和认知指标的估算表达。  |  Fabbri, C., et al. 2022. Mol Genet Genomic Med. 10: e1942. PMID: 35488718
  7. 反复发作的肝肿瘤MAN2A1-FER蛋白缺乏激酶活性：对酪氨酸激酶抑制剂使用的启示。  |  Desaunay, M., et al. 2024. Cell Mol Gastroenterol Hepatol. 17: 667-669. PMID: 38141924
  8. 采用多组学分析了解围产期白介素-6动物模型中肾脏发育的变化。  |  Panzade, G., et al. 2024. Cells. 13: PMID: 39404429
  9. 全外显子组测序揭示了直肠神经内分泌肿瘤进展过程中的新基因组特征和潜在治疗靶点。  |  Xu, S., et al. 2024. Cell Death Dis. 15: 833. PMID: 39548061
  10. 对Friend白血病整合1转录因子作为类风湿性关节炎的新型免疫炎症生物标志物的多级整合研究：生物信息学、临床队列和机制验证的桥梁。  |  Zhang, M., et al. 2025. J Inflamm Res. 18: 3105-3123. PMID: 40059948

订购信息

产品名称产品编号规格价格数量收藏夹

MAN2A1 抗体 (D-5)

sc-377204
200 µg/ml
$316.00

MAN2A1 (D-5): m-IgG Fc BP-HRP 套装

sc-526089
200 µg Ab; 10 µg BP
$354.00

MAN2A1 抗体 (D-5) AC

sc-377204 AC
500 µg/ml, 25% agarose
$416.00

MAN2A1 抗体 (D-5) HRP

sc-377204 HRP
200 µg/ml
$316.00

MAN2A1 抗体 (D-5) FITC

sc-377204 FITC
200 µg/ml
$330.00

MAN2A1 抗体 (D-5) PE

sc-377204 PE
200 µg/ml
$343.00

MAN2A1 抗体 (D-5) Alexa Fluor® 488

sc-377204 AF488
200 µg/ml
$357.00

MAN2A1 抗体 (D-5) Alexa Fluor® 546

sc-377204 AF546
200 µg/ml
$357.00

MAN2A1 抗体 (D-5) Alexa Fluor® 594

sc-377204 AF594
200 µg/ml
$357.00

MAN2A1 抗体 (D-5) Alexa Fluor® 647

sc-377204 AF647
200 µg/ml
$357.00

MAN2A1 抗体 (D-5) Alexa Fluor® 680

sc-377204 AF680
200 µg/ml
$357.00

MAN2A1 抗体 (D-5) Alexa Fluor® 790

sc-377204 AF790
200 µg/ml
$357.00

Is this antibody suitable for flow cytometry if stained by intracellular staining? 

Asked by: Nucake
Thank you for your question. Unconjugated MAN2A1 Antibody (D-5) is only recommended for detection of MAN2A1 of human origin by WB, IP, IF and ELISA. We also offer Anti-MAN2A1 Antibody (D-5) conjugated to either AlexaFluor, phycoerythrin, or FITC for IF, IHC(P) and FCM.
Answered by: Tech Support Europe
Date published: 2024-03-21

What is the recommended starting dilution for Western blot for sc-377204: MAN2A1 (D-5) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from PhDThis antibody had great specificity against human MAN2A1 with a single band in the western blot assay. In addition, the binding affinity of the antibody with human MAN2A1 is very high. This antibody also had really low cross-reactivity against mouse, rat, rabbit origin MAN2A1. This antibody is suitable for IP, IF and ELISA assay.
Date published: 2020-12-29
Rated 1 out of 5 by from Not good for IFI used it for IF on PFA fixed HeLa cells at 1:50 and 1:100 dilution. It failed to stain golgi in both cases.
Date published: 2017-05-30
Rated 5 out of 5 by from good antibodyused this antibody for western blotting of whole cell lysate of a human fibroblast cell line. works well, only one clean band. Also works for IF in methanol fixed cells.
Date published: 2017-03-28
Rated 5 out of 5 by from Produced positive Western blot data of MAN2A1Produced positive Western blot data of MAN2A1 expression in Hep G2 and JAR whole cell lysates. -SCBT QC
Date published: 2014-09-14
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MAN2A1 抗体 (D-5) is rated 4.0 out of 5 by 4.
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