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LXR alpha/beta/NR1H3/NR1H2 Antibody (H-7) - Western Blotting - Image 378668
LXRα/β 抗体 (H-7): sc-377260. THP-1 (A), c4 (B), M1 (C), A549 (D) 和 U-87 MG (E) 全细胞裂解液中 LXRα/β 表达的 Western 印迹分析.
LXRα/β 抗体 (H-7): sc-377260. THP-1 (A), c4 (B), M1 (C), A549 (D) 和 U-87 MG (E) 全细胞裂解液中 LXRα/β 表达的 Western 印迹分析.

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7): sc-377260

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寫評論提問

说明书
  • LXR alpha/beta/NR1H3/NR1H2 抗体 H-7 是小鼠单克隆 IgG1 κ，LXR alpha/beta/NR1H3/NR1H2抗体, 在22篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的LXRβ的C-terminus的氨基酸433-461之间
  • LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和 来源的LXRα and LXRβ; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗LXR alpha/beta/NR1H3/NR1H2抗体（H-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-377260 X, 200 µg/0.1 ml)
  • 关于如何获取LXR alpha/beta/NR1H3/NR1H2 (H-7): sc-377260的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是LXR alpha/beta/NR1H3/NR1H2 Antibody (H-7) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与LXR alpha/beta/NR1H3/NR1H2 Antibody (H-7) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加LXR抗体，包括LXR α和LXR β

LXRα/β抗体（H-7）是一种IgG1 κ小鼠单克隆LXRα/β抗体（也称为LXRα/β抗体、NR1H2/NR1H3抗体或LXRA/LXRB抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源性LXRα/β蛋白。LXRα/β抗体（H-7）既有非偶联型抗LXRα/β抗体形式，也有多种偶联型抗LXRα/β抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。维A酸是维生素A(视黄醇)的代谢产物，被认为是脊椎动物发育和组织分化过程中重要的信号分子。肝X受体(LXRs) α和β与维甲酸X受体(RXR)协同调节肝细胞和巨噬细胞脂质代谢相关基因的表达。RXR是9-顺式维甲酸的受体，与VDR、TR、PPAR以及肝X受体LXRα(也称为RLD-1)、LXRβ和FXR二聚体形成。FXR和LXR属于孤儿受体的一类，因为它们缺乏明确的功能，而LXR则缺乏明确的配体。LXR/ RXR和FXR/RXR异二聚体均保留对9-顺式维甲酸的反应性。LXRα和LXRβ具有相当的序列同源性和多种功能，对相同的内源性和合成配体产生反应，并在维持脂质稳态中发挥重要作用。LXRβ在神经和内分泌组织中广泛表达和富集。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7)

sc-377260
200 µg/ml
$316.00

LXR alpha/beta/NR1H3/NR1H2 (H-7): m-IgGκ BP-HRP 套装

sc-523253
200 µg Ab, 40 µg BP
$354.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) X

sc-377260 X
200 µg/0.1 ml
$316.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) AC

sc-377260 AC
500 µg/ml, 25% agarose
$416.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) HRP

sc-377260 HRP
200 µg/ml
$316.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) FITC

sc-377260 FITC
200 µg/ml
$330.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) PE

sc-377260 PE
200 µg/ml
$343.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor® 488

sc-377260 AF488
200 µg/ml
$357.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor® 546

sc-377260 AF546
200 µg/ml
$357.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor® 594

sc-377260 AF594
200 µg/ml
$357.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor® 647

sc-377260 AF647
200 µg/ml
$357.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor® 680

sc-377260 AF680
200 µg/ml
$357.00

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor® 790

sc-377260 AF790
200 µg/ml
$357.00

LXR alpha/beta/NR1H3/NR1H2 (H-7) 中和勝肽

sc-377260 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-377260 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from LXRα/β (H-7) western blotting liver and brainLXRα/β expression levels were measured in the brain and the liver of both wt and 3xTg-AD mice brain homogenates. 20 micrograms of total proteins were loaded Bloking overnight in 5% BSA in TTBS Antibody diluted 1:500 in BSA 2% in TTBS Good sensitivity and specificity
Date published: 2024-07-17
Rated 5 out of 5 by from 美しいバンドが検出されます。WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 5 out of 5 by from Excellent in WBWestern blotting dilution 1:1000 C2C12 cell lysate specific and very clear band
Date published: 2018-10-05
Rated 2 out of 5 by from CHIP failed with sampleI applied a sample to do the CHIP experiment, the sample was the mouse liver, the ultrasound fragmented between 300-800bp, each sample used 3ug antibody, the results shown in the figure
Date published: 2017-09-12
Rated 4 out of 5 by from working good!I got this two weeks ago and used 1:1000. working good on my WB.
Date published: 2017-07-21
Rated 5 out of 5 by from great featuresWB 1:500 on mouse liver samples,band is very clear
Date published: 2017-05-08
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of LXRα/β expression in HeLa and THP-1 whole cell lysates.
Date published: 2017-02-01
Rated 5 out of 5 by from Worked well for co-IP in mouse macrophagesWe get pretty results in IP assay, our samples are mouse macrophages, The working dilution is 1: 100.
Date published: 2017-01-15
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LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) is rated 4.5 out of 5 by 10.
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