LIMK-1 Antibody (E-7): sc-48346

LIMK-1 Antibody (E-7) is a mouse monoclonal IgG1 antibody that detects LIMK-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LIMK-1, a member of the LIM kinase family, plays a crucial role in regulating actin cytoskeleton dynamics, which is essential for various cellular processes including cell motility, shape, and morphogenesis. By phosphorylating and inhibiting Cofilin, a key actin-binding protein, LIMK-1 stabilizes F-actin structures, thereby influencing cell movement and growth control. This regulation is particularly important in neuronal differentiation, as LIMK-1 has been shown to inhibit PC12 cell differentiation by interfering with downstream signaling events of mitogen-activated protein kinase (MAPK) activation. Expression patterns of LIMK-1 and LIMK-2 suggest distinct roles during development, with LIMK-1 being more prominent in certain tissues and developmental stages. A truncated form of LIMK-2 has been identified in adult testis, arising from an alternative initiation exon, further highlighting LIM kinases′ complexity and functional diversity in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

LIMK-1 Antibody (E-7) References:

1. Abnormal spine morphology and enhanced LTP in LIMK-1 knockout mice.  |  Meng, Y., et al. 2002. Neuron. 35: 121-33. PMID: 12123613
2. CXCL12/CXCR4 signaling counteracts docetaxel-induced microtubule stabilization via p21-activated kinase 4-dependent activation of LIM domain kinase 1.  |  Bhardwaj, A., et al. 2014. Oncotarget. 5: 11490-500. PMID: 25359780
3. Afzelin exhibits anti-cancer activity against androgen-sensitive LNCaP and androgen-independent PC-3 prostate cancer cells through the inhibition of LIM domain kinase 1.  |  Zhu, KC., et al. 2015. Oncol Lett. 10: 2359-2365. PMID: 26622852
4. Phospholipase C-η2 interacts with nuclear and cytoplasmic LIMK-1 during retinoic acid-stimulated neurite growth.  |  Arastoo, M., et al. 2016. Histochem Cell Biol. 145: 163-73. PMID: 26671787
5. Distribution of LIM domain kinase 1 in the olfactory bulb, cerebral cortex, hippocampus, and cerebellum of the App/PS+/- mice.  |  An, L., et al. 2015. Genet Mol Res. 14: 17244-51. PMID: 26681218
6. Dysregulation of LIMK-1/cofilin-1 pathway: A possible basis for alteration of neuronal morphology in experimental cerebral malaria.  |  Simhadri, PK., et al. 2017. Ann Neurol. 82: 429-443. PMID: 28843047
7. MiR-519d-3p suppresses breast cancer cell growth and motility via targeting LIM domain kinase 1.  |  Li, D., et al. 2018. Mol Cell Biochem. 444: 169-178. PMID: 29188531
8. Colchicine reduces platelet aggregation by modulating cytoskeleton rearrangement via inhibition of cofilin and LIM domain kinase 1.  |  Cimmino, G., et al. 2018. Vascul Pharmacol. 111: 62-70. PMID: 30287213
9. LIMK-1 and LIMK-2, two members of a LIM motif-containing protein kinase family.  |  Nunoue, K., et al. 1995. Oncogene. 11: 701-10. PMID: 7651734
10. Comparison of tissue distribution of two novel serine/threonine kinase genes containing the LIM motif (LIMK-1 and LIMK-2) in the developing rat.  |  Mori, T., et al. 1997. Brain Res Mol Brain Res. 45: 247-54. PMID: 9149099

The preferred LIMK-1 antibody is LIMK-1 Antibody (C-10): sc-28370.