Date published: 2026-5-25

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LCAT Antibody (D-3): sc-377509

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Datasheets
  • LCAT Antibody (D-3) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
  • raised against amino acids 283-423 mapping near the C-terminus of LCAT of human origin
  • recommended for detection of precursor and mature LCAT of human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for LCAT Antibody (D-3) for WB applications. These reagents are now offered in bundles with LCAT Antibody (D-3) (see ordering information below).
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    LCAT Antibody (D-3) is a mouse monoclonal IgG2a antibody that detects lecithin-cholesterol acyltransferase (LCAT) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). LCAT plays a vital role in lipid metabolism by catalyzing the esterification of cholesterol, which is essential for cholesterol transport in the bloodstream. This enzymatic activity maintains proper lipid levels and prevents free cholesterol accumulation, which can lead to atherosclerosis and other cardiovascular diseases. Additionally, LCAT is primarily located in plasma, where LCAT interacts with high-density lipoproteins (HDL) to facilitate free cholesterol conversion into cholesteryl esters, promoting reverse cholesterol transport. LCAT deficiencies can result in significant health issues, such as fish-eye disease, a rare genetic disorder characterized by abnormal HDL metabolism, underscoring LCAT′s importance in maintaining cardiovascular health. Anti-LCAT antibody (D-3) serves as an invaluable tool for researchers studying lipid metabolism and related disorders, providing reliable detection and analysis of this critical protein.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    LCAT Antibody (D-3)

    sc-377509
    200 µg/ml
    $322.00

    LCAT Antibody (D-3): m-IgG Fc BP-HRP Bundle

    sc-538048
    200 µg Ab; 10 µg BP
    $361.00

    LCAT Antibody (D-3): m-IgGκ BP-HRP Bundle

    sc-535574
    200 µg Ab; 40 µg BP
    $361.00

    LCAT Antibody (D-3): m-IgG2a BP-HRP Bundle

    sc-548187
    200 µg Ab; 10 µg BP
    $361.00

    What is the recommended starting dilution for Western blot for LCAT (D-3): sc-377509 monoclonal antibody?

    Asked by: Dr Ninau Qelp
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-377509.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2026, m_5, d_24, h_14CST
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    Rated 5 out of 5 by from Produced positive Western blot data of LCATProduced positive Western blot data of LCAT expression in non-transfected and human LCAT transfected whole cell lysates. -SCBT QC
    Date published: 2013-07-29
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    LCAT Antibody (D-3) is rated 5.0 out of 5 by 1.
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