Date published: 2025-9-6
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LAMP1 Antibody (E-5) - Western Blotting - Image 4895
LAMP-1 抗体 (E-5): sc-17768. 对 HeLa (A), JAR (B) 和 ECV304 (C) 全细胞裂解液中 LAMP-1 的表达进行 Western 印迹分析.
LAMP-1 抗体 (E-5): sc-17768. 对 HeLa (A), JAR (B) 和 ECV304 (C) 全细胞裂解液中 LAMP-1 的表达进行 Western 印迹分析.

LAMP1 抗体 (E-5): sc-17768

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寫評論提問

说明书
  • LAMP1抗体(E-5)是小鼠单克隆IgG2a κ, 在94篇文献中引用，规格为200 µg/ml
  • 免疫human物种的LAMP-1 (lysosome-associated membrane glycoprotein-1)的氨基酸1-228
  • 抗-LAMP1 抗体 (E-5) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 LAMP-1
  • 抗LAMP1抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LAMP1 (E-5): sc-17768的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 LAMP1 抗体 (E-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LAMP1 抗体 (E-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 LAMP 抗体 包括 LAMP-1, LAMP-2和LAMP-5

LAMP-1抗体（E-5）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的LAMP-1。抗LAMP-1抗体（E-5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。LAMP-1，也称为CD107a，是一种糖基化的一型膜蛋白，主要位于溶酶体膜上，在维持溶酶体的完整性和功能方面发挥着至关重要的作用。 LAMP-1对于细胞废弃物的正常降解和细胞成分的循环利用至关重要，而细胞成分的循环利用对于细胞稳态至关重要。此外，LAMP-1还参与细胞粘附过程，并促进肿瘤侵袭和转移，这凸显了LAMP-1在正常生理和疾病状态中的重要性。在溶酶体贮积症中，LAMP-1的表达显著增加，这表明LAMP-1可能成为这些疾病的生物标志物。LAMP-1单克隆抗体（E-5）使研究人员能够深入了解溶酶体蛋白在各种生物过程和疾病机制中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LAMP1 抗体 (E-5) 参考文献:

  1. LAMP-1和LAMP-2，但不是LAMP-3，是活化诱导的人肥大细胞分泌的可靠标记物。  |  Grützkau, A., et al. 2004. Cytometry A. 61: 62-8. PMID: 15351990
  2. LAMP-1和LAMP-2在溶酶体生物发生和自噬中的作用。  |  Eskelinen, EL. 2006. Mol Aspects Med. 27: 495-502. PMID: 16973206
  3. FUT1对LAMP-1和LAMP-2的富集作用与乳腺癌细胞的溶酶体定位和自噬通量相关。  |  Tan, KP., et al. 2016. Cell Death Dis. 7: e2347. PMID: 27560716
  4. 溶酶体相关膜蛋白-1和-2（LAMP-1和LAMP-2）通过不同的模式组装。  |  Terasawa, K., et al. 2016. Biochem Biophys Res Commun. 479: 489-495. PMID: 27663661
  5. LAMP-1基因在高等级胶质瘤中过度表达。  |  Sarafian, VS., et al. 2018. APMIS. 126: 657-662. PMID: 29920782
  6. 主要溶酶体膜蛋白 LAMP-1 和 LAMP-2 参与 C2C12 肌母细胞的分化  |  Sakane, H. and Akasaki, K. 2018. Biol Pharm Bull. 41: 1186-1193. PMID: 30068868
  7. 狼疮性肾炎患者的CD107a+（LAMP-1）细胞毒性CD8+ T细胞。  |  Wiechmann, A., et al. 2021. Front Med (Lausanne). 8: 556776. PMID: 33834029
  8. 与 HIV-1 p55Gag 嵌合的 LAMP-1 在新生小鼠免疫中可诱导早期生殖中心形成和 AID 表达。  |  Teixeira, FME., et al. 2022. Vaccines (Basel). 10: PMID: 36016134
  9. 使用深度突变表位图谱和AlphaFold解析LAMP-1抗体的跨物种反应性。  |  Pruvost, T., et al. 2023. MAbs. 15: 2175311. PMID: 36797224

订购信息

产品名称产品编号规格价格数量收藏夹

LAMP1 抗体 (E-5)

sc-17768
200 µg/ml
$316.00

LAMP1 (E-5): m-IgG Fc BP-HRP 套装

sc-525484
200 µg Ab; 10 µg BP
$354.00

LAMP1 (E-5): m-IgG2a BP-HRP 套装

sc-546280
200 µg Ab; 10 µg BP
$354.00

LAMP1 抗体 (E-5) AC

sc-17768 AC
500 µg/ml, 25% agarose
$416.00

LAMP1 抗体 (E-5) HRP

sc-17768 HRP
200 µg/ml
$316.00

LAMP1 抗体 (E-5) FITC

sc-17768 FITC
200 µg/ml
$330.00

LAMP1 抗体 (E-5) PE

sc-17768 PE
200 µg/ml
$343.00

LAMP1 抗体 (E-5) Alexa Fluor® 488

sc-17768 AF488
200 µg/ml
$357.00

LAMP1 抗体 (E-5) Alexa Fluor® 546

sc-17768 AF546
200 µg/ml
$357.00

LAMP1 抗体 (E-5) Alexa Fluor® 594

sc-17768 AF594
200 µg/ml
$357.00

LAMP1 抗体 (E-5) Alexa Fluor® 647

sc-17768 AF647
200 µg/ml
$357.00

LAMP1 抗体 (E-5) Alexa Fluor® 680

sc-17768 AF680
200 µg/ml
$357.00

LAMP1 抗体 (E-5) Alexa Fluor® 790

sc-17768 AF790
200 µg/ml
$357.00

Why would you recommend this mouse monoclonal antibody, sc-17768: LAMP-1 (E-5) over the now discontinued rabbit polyclonal antibody, sc-5570: LAMP-1 (H-228)?

Asked by: cjMara
Thank you for your question. This mouse monoclonal antibody is raised against the identical recombinant protein immunogen used to generate the rabbit polyclonal (sc-5570). This monoclonal antibody offers improved specificity in the same applications, without any lot to lot variability. The antibody has published data in WB, IF, IHC(P) and FCM. And it has been featured in more publications than the polyclonal.
Answered by: Technical Support
Date published: 2016-12-30
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Rated 5 out of 5 by from Leptin signaling impairs macrophage defenses againThe dynamic interplay between metabolism and immune responses in health and disease, by which different immune cells impact on metabolic processes, are being increasingly appreciated. However, the potential of master regulators of metabolism to control innate immunity are less understood. Here, we studied the cross-talk between leptin signaling and macrophage function in the context of bacterial infections. We found that upon infection with Gram-negative pathogens, such as Salmonella Typhimurium, leptin receptor (Lepr) expression increased in both mouse and human macrophages. Unexpectedly, both genetic Lepr ablation in macrophages and global pharmacologic leptin antagonization augmented lysosomal functions, reduced S Typhimurium burden, and diminished inflammation in vitro and in vivo. Mechanistically, we show that leptin induction activates the mTORC2/Akt pathway and subsequently down-regulates Phlpp1 phosphatase, allowing for phosphorylated Akt to impair lysosomal-mediated pathogen clearance. These data highlight a link between leptin signaling, the mTORC2/Phlpp1/Akt axis, and lysosomal activity in macrophages and have important therapeutic implications for modulating innate immunity to combat Gram-negative bacterial infections.
Date published: 2021-08-26
Rated 1 out of 5 by from 3Dマトリジェル内の細胞染色には不向き？ホルマリン＋グルタルアルデヒド固定した3Dマトリジェル内の培養細胞に対して免疫染色したが、LAMP-1抗体(H4A3)に比べて綺麗に染まらなかった。
Date published: 2021-05-19
Rated 5 out of 5 by from sc-17768sc-17768 worked great for IHC and I highly recommend this product
Date published: 2019-12-04
Rated 5 out of 5 by from 很好！前几天申请了试用装，做免疫印迹用，肝癌细胞系（HepG2)的裂解液，稀释比例为1：500，结果挺好的，推荐使用！
Date published: 2018-01-25
Rated 5 out of 5 by from 功能出色Strong cytoplasm localization in IF in methanol-fixed monocyte-derived macrophages
Date published: 2017-11-02
Rated 5 out of 5 by from Fantastic!Produces strong, robust signal in Western blot and IHC. Great antibody
Date published: 2017-01-05
Rated 5 out of 5 by from Worked well in WB in human MDAWorked well in WB in human MDA-MB-231 cells, hTERT-BJ1 cells. -SCBT Publication Review
Date published: 2015-04-12
Rated 5 out of 5 by from Strong cytoplasm localization in IF in methanolStrong cytoplasm localization in IF in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-01
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LAMP1 抗体 (E-5) is rated 4.7 out of 5 by 12.
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