Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Laminin β-1 Double Nickase Plasmid (h): sc-401105-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Laminin β-1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Laminin β-1 Double Nickase Plasmid (h) and Laminin β-1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LAMB1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Laminin β-1 Antibody (A-1): sc-17810
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Laminin β-1 Double Nickase Plasmid (h) | sc-401105-NIC | 20 µg | $410.00 | |||
Laminin β-1 Double Nickase Plasmid (h2) | sc-401105-NIC-2 | 20 µg | $410.00 |
LAMB1 encodes laminin β-1, a core subunit of multiple laminin heterotrimers that assemble into basement membranes and organize extracellular matrix architecture. Through interactions with integrins, dystroglycan, and other matrix components, laminin β-1 supports cell adhesion, polarity, migration, and survival, influencing signaling nodes such as focal adhesion kinase and PI3K/AKT as well as cytoskeletal dynamics. In human tissues, LAMB1 contributes to epithelial and endothelial barrier properties and guides developmental processes including neurite outgrowth and tissue morphogenesis. Altered laminin composition or disrupted basement membrane assembly involving LAMB1 has been associated with congenital structural defects and can modulate invasive and fibrotic phenotypes in disease-relevant models.
Laminin β-1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LAMB1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LAMB1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LAMB1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LAMB1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.