Laemmli Sample Buffer (odor-free), 2X A sample buffer formulated for protein samples and used in the Laemmli SDS-PAGE system.

Laemmli Sample Buffer (odor-free), 2X

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应用; A sample buffer formulated for protein samples and used in the Laemmli SDS-PAGE system
* 参考分析证明 大量特定数据 (包括水 含量).
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Laemmli Sample Buffer (odor-free), 2X is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The DTT reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.


参考文献

1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Wu, M., et al. 2009. Diabetes Metab. Res. Rev. 25: 279-286. PMID: 19177471
3. Wu, M., et al. 2009. PLoS ONE. 4: e6430. PMID: 19641606

用法 :
Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.
规划 :
2X solution contains 4% SDS, 20% glycerol, 10% 400 mM DTT, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8.
物理状态 :
Liquid
pH :
~6.8
保存 :
Store at -20° C
仅供科研使用。不可用于诊断或治疗。

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