You may not log in without verifying your email address.
Please check your email (including your spam email) for the Welcome message that was sent.
Laemmli Sample Buffer (odor-free), 2X is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The DTT reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well.
1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Wu, M., et al. 2009. Diabetes Metab. Res. Rev. 25: 279-286. PMID: 19177471
3. Wu, M., et al. 2009. PLoS ONE. 4: e6430. PMID: 19641606
圣克鲁斯生物技术在生物医药研究市场的产品开发方面处于世界领先地位。欢迎 致电我们的免费电话1-800-457-3801。 版权 ©, 2007-2020圣克鲁斯生物技术。版权所有。“Santa Cruz Biotechnology”，圣克鲁斯生物技 术的 商标，“Santa Cruz Animal Health”，“San Juan Ranch”，“Supplement of Champions”，San Juan Ranch的商标，“Ultracruz”，“Chemcruz”，“Immunocruz”，“Exactacruz”和“EZ Touch”都是圣克鲁斯生物技术的注册商标。所有的商标都归其各自拥有者所有。