Date published: 2025-9-9
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lactoferrin Antibody (B97) - Western Blotting - Image 12629
乳铁蛋白抗体 (B97): sc-53498. 人 PBL 全细胞裂解液中乳铁蛋白表达的 Western 印迹分析.
乳铁蛋白抗体 (B97): sc-53498. 人 PBL 全细胞裂解液中乳铁蛋白表达的 Western 印迹分析.

lactoferrin 抗体 (B97): sc-53498

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说明书
  • lactoferrin 抗体 B97 是小鼠单克隆 IgG1 κ，lactoferrin抗体, 在5篇文献中引用，规格为200 µg/ml
  • 全长纯化 human lactoferrin
  • lactoferrin 抗体 (B97) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA，检测human 来源的 lactoferrin
  • 抗lactoferrin抗体（B97）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取lactoferrin (B97): sc-53498的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 lactoferrin 抗体 (B97) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与lactoferrin 抗体 (B97) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

乳铁蛋白抗体（B97）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测人类乳铁蛋白。抗乳铁蛋白抗体（B97）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。乳铁蛋白是转铁蛋白家族的一员，通过结合铁并调节其可用性在铁代谢中发挥关键作用，这对包括免疫反应和细胞生长在内的各种生物过程至关重要。乳铁蛋白主要存在于牛奶、眼泪和白细胞中，在这些地方，乳铁蛋白不仅是一种铁结合蛋白，还具有抗菌特性。乳铁蛋白能够降解流感嗜血杆菌分泌的IgA1蛋白酶，从而增强人体IgA1抗体预防细菌定植的功效。乳铁蛋白能够通过干扰病毒融合和进入宿主细胞来抑制HIV-1复制，这凸显了其潜在的治疗应用。编码人乳铁蛋白的基因位于3p21.31号染色体上，乳铁蛋白的表达受各种生理和病理条件的影响，因此乳铁蛋白是免疫学研究中的重要生物标志物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

lactoferrin 抗体 (B97) 参考文献:

  1. 人类乳铁蛋白及其 N 端衍生物肽对抗生素耐药菌感染非常有效。  |  Nibbering, PH., et al. 2001. Infect Immun. 69: 1469-76. PMID: 11179314
  2. 一种牛奶蛋白乳铁蛋白能增强人类 T 细胞白血病病毒 I 型并抑制 HIV-1 感染。  |  Moriuchi, M. and Moriuchi, H. 2001. J Immunol. 166: 4231-6. PMID: 11238676
  3. 乳铁蛋白可调节人外周血单核细胞对植物血凝素和混合淋巴细胞反应的增殖反应。  |  Zimecki, M., et al. 2001. Arch Immunol Ther Exp (Warsz). 49: 147-54. PMID: 11348019
  4. 乳铁蛋白与宿主防御  |  Ward, PP., et al. 2002. Biochem Cell Biol. 80: 95-102. PMID: 11908649
  5. 无菌性和化脓性炎症中的乳铁蛋白  |  Lepanto, MS., et al. 2019. Molecules. 24: PMID: 30987256
  6. 利用乳铁蛋白纳米粒子靶向癌症：最新进展。  |  Kondapi, AK. 2020. Nanomedicine (Lond). 15: 2071-2083. PMID: 32779524
  7. 乳铁蛋白和脑内出血后血肿的解毒作用。  |  Zhao, X., et al. 2021. Biochem Cell Biol. 99: 97-101. PMID: 32886889
  8. 影响母乳乳铁蛋白的因素。  |  Briana, DD. and Papaevangelou, V. 2022. J Matern Fetal Neonatal Med. 35: 8111. PMID: 34362273
  9. 将乳转铁蛋白基因分配到人类第 3 号染色体和小鼠第 9 号染色体上。  |  Teng, CT., et al. 1987. Somat Cell Mol Genet. 13: 689-93. PMID: 3478818
  10. 乳铁蛋白在眼表的生物医学应用。  |  Regueiro, U., et al. 2023. Pharmaceutics. 15: PMID: 36986726
  11. 铁运输和储存蛋白  |  Aisen, P. and Listowsky, I. 1980. Annu Rev Biochem. 49: 357-93. PMID: 6996567
  12. 基于单克隆抗体的人类乳铁蛋白免疫测定。  |  Chung, S., et al. 1985. J Immunol Methods. 84: 135-41. PMID: 2415634

订购信息

产品名称产品编号规格价格数量收藏夹

lactoferrin 抗体 (B97)

sc-53498
200 µg/ml
$316.00

lactoferrin (B97): m-IgG Fc BP-HRP 套装

sc-526820
200 µg Ab; 10 µg BP
$354.00

lactoferrin (B97): m-IgG1 BP-HRP 套装

sc-532193
200 µg Ab; 20 µg BP
$354.00

lactoferrin 抗体 (B97) AC

sc-53498 AC
500 µg/ml, 25% agarose
$416.00

lactoferrin 抗体 (B97) HRP

sc-53498 HRP
200 µg/ml
$316.00

lactoferrin 抗体 (B97) FITC

sc-53498 FITC
200 µg/ml
$330.00

lactoferrin 抗体 (B97) PE

sc-53498 PE
200 µg/ml
$343.00

lactoferrin 抗体 (B97) Alexa Fluor® 488

sc-53498 AF488
200 µg/ml
$357.00

lactoferrin 抗体 (B97) Alexa Fluor® 546

sc-53498 AF546
200 µg/ml
$357.00

lactoferrin 抗体 (B97) Alexa Fluor® 594

sc-53498 AF594
200 µg/ml
$357.00

lactoferrin 抗体 (B97) Alexa Fluor® 647

sc-53498 AF647
200 µg/ml
$357.00

lactoferrin 抗体 (B97) Alexa Fluor® 680

sc-53498 AF680
200 µg/ml
$357.00

lactoferrin 抗体 (B97) Alexa Fluor® 790

sc-53498 AF790
200 µg/ml
$357.00

Can anti-human anti-lactoferrin sc-53498 monoclonal antibody be used for goat lactoferrin detection?

Asked by: DrMercaptych
Thank you for your question. We do not know if this antibody would recognize lactoferrin of goat origin. This is a monoclonal antibody raised against full length purified lactoferrin of human origin. The exact epitope recognized by this monoclonal antibody has not been determined. The full length human lactoferrin protein sequence does share ~71% sequence identity with the goat lactoferrin protein sequence. This is not great enough sequence identity for us to guarantee cross-reactivity, but there is certainly a chance that the antibody may cross-react with the goat protein.
Answered by: Technical Support
Date published: 2019-02-05

Can lactoferrin (B97): sc-53498 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, lactoferrin (B97): sc-53498 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Is ist possible, that the antibody detects fragments of LT at 40 and 35 kD?

Asked by: Simon
  • y_2025, m_8, d_27, h_5CST
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Rated 1 out of 5 by from Kein Signal im Western-BlotIch habe verschiedene Zelllysate bei einem Einsatz von je 30µg getestet: Hela; HEPG2; MDA-MB231 Sowohl die 1:500 als auch die 1:250 Verdünnung gaben kein Signal.
Date published: 2017-10-16
Rated 5 out of 5 by from Very good antibodyVery good antibody, I would recommend my friends to use.
Date published: 2017-07-15
Rated 5 out of 5 by from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, Flow Cytometry, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-24
Rated 5 out of 5 by from Performs well in WesternPerforms well in Western, good signal with human PBL whole cell lysate. -SCBT QC
Date published: 2015-01-09
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human liver tissue. -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from Great indirectGreat indirect, intracellular FCM data of fixed and permeabilized human peripheral blood leukocytes stained with lactoferrin . -SCBT QC
Date published: 2013-09-21
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lactoferrin 抗体 (B97) is rated 4.3 out of 5 by 6.
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