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Janus Green B (CAS 2869-83-2)

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Alternate Names:
Diazin Green S
Application:
Janus Green B is a stain used for histology studies of mitochondria
CAS Number:
2869-83-2
Molecular Weight:
511.06
Molecular Formula:
C30H31N6•Cl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Janus Green B is a stain that interacts with DNA and has been used for histology studies for mitochondrial staining, in which its oxidation and reduction reveals alterations to the electron transfer chain. The stain has been utilized in studies for effective and rapid staining of insect chordotonal organs and peripheral nerves, where the nerves appear dark blue in comparison to non stained tissue. With the use of Janus Green B, rabbit lymphatic vessel studies demonstrate that threadlike structures within the lymphatic vessels, known as Bonghan ducts, contain a high density of mitochondria in their cells.


Janus Green B (CAS 2869-83-2) References

  1. Interactions of Janus Green B with double stranded DNA and the determination of DNA based on the measurement of enhanced resonance light scattering.  |  Huang, CZ., et al. 2000. Analyst. 125: 1267-72. PMID: 10984922
  2. Studies on the mechanism of Janus green B staining of mitochondria. IV. Reduction of Janus green B by isolated cell fractions.  |  COOPERSTEIN, SJ., et al. 1960. Anat Rec. 138: 49-66. PMID: 13695346
  3. Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method.  |  Lee, BC., et al. 2005. Anat Rec B New Anat. 286: 1-7. PMID: 16177995
  4. René Couteaux (1909-1999) and the morphological identification of synapses.  |  Tsuji, S. 2006. Biol Cell. 98: 503-9. PMID: 16842240
  5. Using Janus green B to study paraquat toxicity in rat liver mitochondria: role of ACE inhibitors (thiol and nonthiol ACEi).  |  Ghazi-Khansari, M., et al. 2006. Ann N Y Acad Sci. 1090: 98-107. PMID: 17384251
  6. Flow cytometric identification of two different rhodamine-123-stained mitochondrial populations in maize leaves.  |  Yang, J., et al. 2007. Protoplasma. 231: 249-52. PMID: 17922268
  7. Comparative analyses of cell disruption methods for mitochondrial isolation in high-throughput proteomics study.  |  Chaiyarit, S. and Thongboonkerd, V. 2009. Anal Biochem. 394: 249-58. PMID: 19622339
  8. Photo-Physical Characteristics of Janus Green B in Different Solvents and its Interaction Mechanism with Silver Nanoparticles.  |  Gawad, SAA., et al. 2024. J Fluoresc.. PMID: 38773030
  9. Janus Green B as a rapid, vital stain for peripheral nerves and chordotonal organs in insects.  |  Yack, JE. 1993. J Neurosci Methods. 49: 17-22. PMID: 7505866
  10. Binding of the cationic dye, Janus green B, as a measure of the specific surface area of crystalline silica in aqueous suspension.  |  Daniel, LN., et al. 1993. Toxicol Appl Pharmacol. 123: 62-7. PMID: 8236262

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Janus Green B, 5 g

sc-203740
5 g
$64.00

Janus Green B, 25 g

sc-203740A
25 g
$207.00

Janus Green B, 100 g

sc-203740B
100 g
$693.00

Janus Green B, 250 g

sc-203740C
250 g
$1359.00