Date published: 2025-9-7
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ISGF-3γ p48 Antibody (H-10) - Western Blotting - Image 300148
ISGF-3γ p48 抗体 (H-10): sc-365893. 未转染 293T: sc-117752 (A), 人 ISGF-3γ p48 转染 293T: sc-115734 (B), Hep G2 (C), HeLa (D) 和 A549 (E) 全细胞裂解液中 ISGF-3γ p48 表达的 Western 印迹分析.
ISGF-3γ p48 抗体 (H-10): sc-365893. 未转染 293T: sc-117752 (A), 人 ISGF-3γ p48 转染 293T: sc-115734 (B), Hep G2 (C), HeLa (D) 和 A549 (E) 全细胞裂解液中 ISGF-3γ p48 表达的 Western 印迹分析.

ISGF-3γ p48 抗体 (H-10): sc-365893

4.8(10)
寫評論提問

说明书
  • ISGF-3 gamma p48 抗体 H-10 是小鼠单克隆 IgG1 κ，ISGF-3γ p48抗体, 在13篇文献中引用，规格为200 µg/ml
  • 免疫human物种的ISGF-3γ p48的氨基酸113-255
  • ISGF-3 gamma p48 抗体 (H-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 ISGF-3γ p48
  • 抗ISGF-3 gamma p48抗体（H-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-365893 X, 200 µg/0.1 ml)
  • 关于如何获取ISGF-3γ p48 (H-10): sc-365893的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 ISGF-3γ p48 抗体 (H-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ISGF-3γ p48 抗体 (H-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

ISGF-3γ p48抗体（H-10）是一种IgG1κ小鼠单克隆ISGF-3 gamma p48抗体（也称为干扰素调节因子9抗体、干扰素刺激转录因子3 Gamma 48kDa抗体、IFN-α响应转录因子亚基抗体、转录调节因子ISGF3亚基Gamma抗体、干扰素刺激基因因子3 Gamma抗体、ISGF3 P48亚基抗体、ISGF-3 Gamma抗体、ISGF3G抗体、IRF-9抗体、ISGF3抗体或P48抗体），通过WB、IP、IF、IHC（P）和ELISA检测人源ISGF-3 gamma p48蛋白。ISGF-3γ p48抗体（H-10）既有非偶联的抗ISGF-3 gamma p48抗体形式，也有多种偶联形式的抗ISGF-3 gamma p48抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。干扰素信号传导到细胞核是通过蛋白质酪氨酸的磷酸化来实现的，这些蛋白质被称为Stat（信号转导子和转录激活子）。该家族中第一个被描述的成员包括Stat1α p91、Stat1β p84（一种缺少38个COOH末端氨基酸的p91）和Stat2 p113。该家族的其他成员包括Stat3，它通过酪氨酸磷酸化被激活，作为DNA结合蛋白，响应表皮生长因子（EGF）和白细胞介素-6（IL-6），但不响应干扰素γ（IFN-γ）和Stat4。Stat1α p91（或Stat1β p84）和p113与p48形成复合物（称为ISGF-3），p48是一种蛋白质，序列分析表明它是干扰素调节（IRF）DNA结合蛋白家族的成员。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ISGF-3γ p48 抗体 (H-10) 参考文献:

  1. 人类干扰素调节因子 1（IRF-1）和 IRF-2 基因的结构和调控：对干扰素系统基因网络的影响。  |  Harada, H., et al. 1994. Mol Cell Biol. 14: 1500-9. PMID: 7507207
  2. 参与 gp130 介导的信号通路的新型 IFN 刺激基因因子 3 p91 相关转录因子 APRF 的分子克隆。  |  Akira, S., et al. 1994. Cell. 77: 63-71. PMID: 7512451
  3. γ干扰素激活基因所需的 Stat91 单个磷酸酪氨酸残基。  |  Shuai, K., et al. 1993. Science. 261: 1744-6. PMID: 7690989
  4. Stat4，一种在早期骨髓分化过程中表达的新型γ干扰素激活位点结合蛋白。  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
  5. Stat3：一种 STAT 家族成员，在表皮生长因子和白细胞介素-6 的作用下通过酪氨酸磷酸化激活。  |  Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
  6. Jak-STAT 通路和转录激活对 IFNs 及其他细胞外信号蛋白的反应。  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455
  7. 转录因子 p91 与表皮生长因子受体相互作用，并介导 c-fos 基因启动子的激活。  |  Fu, XY. and Zhang, JJ. 1993. Cell. 74: 1135-45. PMID: 8402883

订购信息

产品名称产品编号规格价格数量收藏夹

ISGF-3γ p48 抗体 (H-10)

sc-365893
200 µg/ml
$316.00

ISGF-3γ p48 (H-10): m-IgG Fc BP-HRP 套装

sc-529500
200 µg Ab; 10 µg BP
$354.00

ISGF-3γ p48 (H-10): m-IgGκ BP-HRP 套装

sc-522408
200 µg Ab, 40 µg BP
$354.00

ISGF-3γ p48 (H-10): m-IgG1 BP-HRP 套装

sc-543545
200 µg Ab; 20 µg BP
$354.00

ISGF-3γ p48 抗体 (H-10) X

sc-365893 X
200 µg/0.1 ml
$316.00

ISGF-3γ p48 抗体 (H-10) AC

sc-365893 AC
500 µg/ml, 25% agarose
$416.00

ISGF-3γ p48 抗体 (H-10) HRP

sc-365893 HRP
200 µg/ml
$316.00

ISGF-3γ p48 抗体 (H-10) FITC

sc-365893 FITC
200 µg/ml
$330.00

ISGF-3γ p48 抗体 (H-10) PE

sc-365893 PE
200 µg/ml
$343.00

ISGF-3γ p48 抗体 (H-10) Alexa Fluor® 488

sc-365893 AF488
200 µg/ml
$357.00

ISGF-3γ p48 抗体 (H-10) Alexa Fluor® 546

sc-365893 AF546
200 µg/ml
$357.00

ISGF-3γ p48 抗体 (H-10) Alexa Fluor® 594

sc-365893 AF594
200 µg/ml
$357.00

ISGF-3γ p48 抗体 (H-10) Alexa Fluor® 647

sc-365893 AF647
200 µg/ml
$357.00

ISGF-3γ p48 抗体 (H-10) Alexa Fluor® 680

sc-365893 AF680
200 µg/ml
$357.00

ISGF-3γ p48 抗体 (H-10) Alexa Fluor® 790

sc-365893 AF790
200 µg/ml
$357.00

I am using ISGF-3γ p48 (H-10): sc-365893 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great for WB!I am using this antibody to do WB and check endogenous IRF9 level before and after IFNalpha and IFNgamma treatment in Huh7.5, 2fTGH, A549 and HeLa cells. Nice clear one band. I am using SnapID system and 1:500 in 2ml 1%BSA for 30min. 30ug protein per lane. Semi-dry transfer. I did one trial for ChIP but the results were not good (1ug of ab per chromatin from 3x10^6 cells)
Date published: 2021-10-28
Rated 5 out of 5 by from Very clear signalThis antibody works well in western blot of whole cell extracts prepared from several human cell lines.
Date published: 2021-05-31
Rated 3 out of 5 by from It works for western blotting.It detected endogenous IRF9 in total protein extracts from immortalized HFFs (1:500 dilution in 5% milk/TBS-0.1% Tween-20, overnight at 4C), although the band was faint.
Date published: 2018-02-15
Rated 5 out of 5 by from good1:500 use,have a clear band in bv2 but not detect in hela,why？
Date published: 2017-10-21
Rated 5 out of 5 by from well performanceThis antibody is widely used for detect ISGF-3γ p48 protein,1:500 used for human liver cancer tissue lysates(RIPA Buffer)
Date published: 2017-07-01
Rated 5 out of 5 by from specific antibodyStrong single band in WB in mouse Peritoneal macrophages,good specificity.
Date published: 2017-05-13
Rated 5 out of 5 by from Works as expected for WBStrong clean signal in WB on Hep G2 and Jurkat whole cell lysates at 1:200 dilution.
Date published: 2017-01-30
Rated 5 out of 5 by from The effect was goodThe results of Western blot analysis showed that isgf-3 P48 expression of MCF7 cell lysate. The effect was good, clear and powerful in WB.
Date published: 2017-01-11
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ISGF-3γ p48 抗体 (H-10) is rated 4.8 out of 5 by 10.
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