ISG15抗体 (F-9) 推荐用于 mouse, rat 和 human 来源的ISG15 WB, IP, IF, IHC(P) 和 ELISA检测

ISG15 抗体 (F-9): sc-166755

ISG15抗体 (F-9) is rated 4.5 out of 5 by 24.
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说明书

    • 抗-ISG15 抗体 F-9 是小鼠单克隆 IgG1 κ,ISG15抗体, 在57篇文献中引用,规格为200 µg/ml
    • raised against amino acids 1-150 mapping at the N-terminus of ISG15 of human origin
    • 抗-ISG15 抗体 (F-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 ISG15
    • 抗-ISG15 抗体 (F-9) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取ISG15 (F-9): sc-166755免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 ISG15 Antibody (F-9) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供ISG15 Antibody (F-9)。 (见下方订购信息).
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-The datasheet specifies this is "a mouse monoclonal antibody raised against amino acids 1-150" but ISG15 is 165 aa. Is there more information on the epitope (not highlighted in the protein sequence tab)? -5% milk in TBS 0.1% Tween 4 C overnighincubation?

Asked by: autumnos
Thank you for your question. Yes, this information is correct, the antibody is raised against a recombinant protein corresponding to amino acids 1-150 of ISG15 of human origin. The exact epitope recognized by this monoclonal antibody has not been determined. We do recommend blocking in 5% nonfat milk in TBST (0.05% Tween-20) for one hour at room temperature, and incubating the primary antibody at the starting dilution of 1:200 in 5% nonfat milk + TBST for one hour at room temperature, or overnight at 4C.
Answered by: Technical Support
Date published: 2019-07-03

What is the light chain of ISG15 Antibody (F-9): sc-166755 ?

Asked by: cjMara
ISG15 Antibody (F-9) is a mouse monoclonal IgG1 with kappa light chain.
Answered by: Technical Support 9
Date published: 2017-09-13
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Rated 4 out of 5 by from Very good ISG15 antibody It works really well, single band, strong. No background. Samples are liver homogenates from bacteria-infected mice.
Date published: 2021-06-04
Rated 4 out of 5 by from Works in Western Clean and sharp band observed by Western blotting at expected height; worked well in lysates of various cell lines; as expected, higher expression upon IFN stimulation
Date published: 2020-09-28
Rated 5 out of 5 by from The antibody is good I got a free test antibody, the antibody is very good, it also can be used for IP.
Date published: 2019-02-26
Rated 5 out of 5 by from Strong signal F-9 antibody produces a strong signal on WB at 1:1000 dilution. The band observed is a bit "lighter" than expected but that's probably caused by using Tris-Glycine system.
Date published: 2018-02-02
Rated 5 out of 5 by from sc-166755 This antibody works great for WB (cell lysates; mouse)
Date published: 2018-01-02
Rated 5 out of 5 by from Nice IHC staining I used it for IHC of human cells an gives an intense and specific signal.
Date published: 2017-12-22
Rated 5 out of 5 by from Works good in WB This Ab with 1:1000 dilution was used for the detection of human samples and the interested band could be observed clearly in WB image.
Date published: 2017-09-28
Rated 5 out of 5 by from an excellent antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
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