Date published: 2025-9-6

021-6093-6350

SCBT Portrait Logo
Seach Input

Intersectin 抗体 (29): sc-136242

4.3(3)
寫評論提問

说明书
  • Intersectin 抗体 29 是小鼠单克隆 IgG1 (kappa轻链),Intersectin抗体,规格为200 µg/ml
  • 免疫mouse物种的Intersectin的氨基酸800-909
  • 推荐用于检测mouse, rat和human来源的Intersectin,由WBIP提供
  • 关于如何获取Intersectin (29): sc-136242免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Intersectin Antibody (29) for WB applications. 的首选辅助检测试剂,这些试剂现与Intersectin Antibody (29) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

Intersectin抗体(29)是一种IgG1 κ小鼠单克隆Intersectin抗体(也被称为Intersectin抗体),能够通过WB和IP检测小鼠、大鼠和人类来源的Intersectin蛋白。Intersectin抗体(29)以非偶联的抗Intersectin抗体形式提供。Intersectin也被称为Ese1或ITSN1,是细胞内吞机制的一个组成部分。Intersectin由两个N端Ese15同源(EH)结构域、一个中央高电荷区域和五个C端SH3结构域组成,这些都在很大程度上有助于Intersectin与内吞途径的其他组分的关联。EH结构域特别负责将Intersectin定向定位到靠近质膜的网格蛋白包被凹坑。在内吞囊泡内,SH3结构域促进Intersectin与Dynamin的结合,而中央结构域对于Intersectin与SNAP 25的关联至关重要。由于在一个终止密码子中的选择性剪接,产生了两种Intersectin的同种型,它们被命名为Intersectin-short和Intersectin-long(或IntersectinS和IntersectinL),以反映出一个延长的C端结构域。具有延长C端结构域的长形式在神经元中特异性表达;短形式在胶质细胞和非神经元细胞中均可检测到。相关蛋白质Intersectin-2和小鼠同源物Ese2也包含特征性的N端EH结构域、中央卷曲螺旋结构域和五个C端SH3结构域,并可能参与内吞支架复合物的形成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Intersectin 抗体 (29) 参考文献:

  1. EH和SH3结构域的Ese蛋白通过与dynamin和Eps15连接来调节内吞。  |  Sengar, AS., et al. 1999. EMBO J. 18: 1159-71. PMID: 10064583
  2. 含 SH3 域的蛋白在凝集素包被囊泡形成的不同步骤中发挥作用。  |  Simpson, F., et al. 1999. Nat Cell Biol. 1: 119-24. PMID: 10559884
  3. 内细胞蛋白 intersectin 是大鼠大脑中 Ras 交换因子 mSos1 的主要结合伙伴。  |  Tong, XK., et al. 2000. EMBO J. 19: 1263-71. PMID: 10716926

订购信息

产品名称产品编号规格价格数量收藏夹

Intersectin 抗体 (29)

sc-136242
200 µg/ml
$316.00

Intersectin (29): m-IgG Fc BP-HRP 套装

sc-539903
200 µg Ab; 10 µg BP
$354.00

Intersectin (29): m-IgG1 BP-HRP 套装

sc-541776
200 µg Ab; 20 µg BP
$354.00

What is the recommended concentration for primary antibody incubation?

Asked by: yl202111
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext.1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-10-11

How much sample do you recommend loading on the gel for Western blot detection using Intersectin (29): sc-136242?

Asked by: Cweed
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_136242, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 112ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good for Western BlotThis antibody works well in NTERA-2 cl.D1, NCI-H266, and NIH/3T3 whole cell lysates and mouse and rat brain tissue extracts. -SCBT QC
Date published: 2023-09-14
Rated 4 out of 5 by from WB for brain tissueGood for WB with band in the right place. Low background.
Date published: 2020-01-07
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_2
  • loc_zh_CN, sid_136242, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 13ms
  • REVIEWS, PRODUCT
Intersectin 抗体 (29) is rated 4.3 out of 5 by 3.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_2
  • loc_zh_CN, sid_136242, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 110ms
  • REVIEWS, PRODUCT