insulin R alpha抗体 (MA-20) recommended for detection of insulin Rα of mouse, rat and human origin by IP

insulin Rα 抗体 (MA-20): sc-57344

insulin Rα抗体 (MA-20) is rated 4.7 out of 5 by 3.
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说明书

    • insulin Rα抗体(MA-20)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
    • raised against placental insulin Rα of human oirgin
    • insulin R alpha 抗体 (MA-20) 推荐用于IP,检测mouse, rat 和 human 来源的insulin Rα
    • 抗-insulin R alpha 抗体 (MA-20) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取insulin Rα (MA-20): sc-57344免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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Are there any issues with using sc-57344: insulin Rα (MA-20) mouse monoclonal primary antibody on mouse samples?

Asked by: jenni
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-57344 conjugates.
Answered by: Technical Support
Date published: 2019-04-25
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Rated 5 out of 5 by from Got target band We tried the antibody dilution range from 1:300 to 1:800.We can get 125kda target band, The high conceration 1:300 may cause background and non specific bands, I suggested that the concentration is around 1:500, the exposure time should not be too long, we recommend controled in 3min,also I highly recommend do internal control on the same membrance at the same time to test the experiment conditions.
Date published: 2017-09-10
Rated 5 out of 5 by from Popular antibody! This is a popular clone for insulin R alpha detection. Great choice!
Date published: 2017-02-07
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