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IL-1α Antibody (ALF-161) - Western Blotting - Image 2611 — IL-1α (ALF-161): sc-12741. 小鼠重组 IL-1α 的 Western 印迹分析.

IL-1α (ALF-161): sc-12741. 小鼠重组 IL-1α 的 Western 印迹分析.

IL-1α 抗体 (ALF-161): sc-12741

Name: IL-1&alpha;抗体 (ALF-161)
Brand: SCBT
SKU: sc-12741
Price: 316.0 USD
Availability: InStock

4.0(4)

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说明书

IL-1α抗体（ALF-161）是一种亚美尼亚仓鼠单克隆 IgG IL-1α抗体，被引用于8篇文献，发布于200 µg/ml
针对全长纯化的mouse来源的重组IL-1α
IL-1 alpha 抗体 (ALF-161) 推荐用于 WB, IP, IF, FCM 和 ELISA，检测mouse, rat 和human 来源的 IL-1α
抗IL-1 alpha抗体（ALF-161）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
还提供无叠氮化物的中和，sc-12741 L，200 µg/0.1 ml
关于如何获取IL-1α (ALF-161): sc-12741的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
目前，我们还没有完成IL-1α 抗体 (ALF-161)的首选二抗检测试剂的鉴定。这项工作正在进行中。

查看产品引用文献(8)

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関連項目

此外 IL-1 抗体包括 IL-1 α, IL-1 β, IL-1F5和IL-1F9

IL-1α抗体（ALF-161）是一种仓鼠单克隆IgG抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的IL-1α。IL-1α抗体（ALF-161）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。IL-1α在免疫反应和炎症中起着关键作用，它作为关键介质，影响T和B淋巴细胞以及自然杀伤（NK）细胞的活性。IL-1α主要位于细胞质中，可在细胞激活时释放，这对于调节炎症反应至关重要。IL-1α在细胞质中的存在使其能够在炎症刺激期间快速释放，从而实现免疫细胞与周围组织之间的快速沟通。IL-1α参与多种生理过程，包括肾脏发育和软骨退化，并涉及多种病理状况，如类风湿性关节炎、炎症性肠病和动脉粥样硬化。尽管IL-1α和IL-1β的遗传起源不同，但它们与相同的受体结合，这凸显了IL-1α在协调免疫反应中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
IL-1α 抗体 (ALF-161)	sc-12741	200 µg/ml	$316.00
IL-1α 抗体 (ALF-161) L	sc-12741 L	200 µg/0.1 ml	$316.00
IL-1α 抗体 (ALF-161) AC	sc-12741 AC	500 µg/ml, 25% agarose	$416.00
IL-1α 抗体 (ALF-161) HRP	sc-12741 HRP	200 µg/ml	$316.00
IL-1α 抗体 (ALF-161) FITC	sc-12741 FITC	200 µg/ml	$330.00
IL-1α 抗体 (ALF-161) PE	sc-12741 PE	200 µg/ml	$343.00
IL-1α 抗体 (ALF-161) Alexa Fluor^® 488	sc-12741 AF488	200 µg/ml	$357.00
IL-1α 抗体 (ALF-161) Alexa Fluor^® 546	sc-12741 AF546	200 µg/ml	$357.00
IL-1α 抗体 (ALF-161) Alexa Fluor^® 594	sc-12741 AF594	200 µg/ml	$357.00
IL-1α 抗体 (ALF-161) Alexa Fluor^® 647	sc-12741 AF647	200 µg/ml	$357.00
IL-1α 抗体 (ALF-161) Alexa Fluor^® 680	sc-12741 AF680	200 µg/ml	$357.00
IL-1α 抗体 (ALF-161) Alexa Fluor^® 790	sc-12741 AF790	200 µg/ml	$357.00

Can IL-1α (ALF-161): sc-12741 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IL-1α (ALF-161): sc-12741 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by 31796433 from Mutant BRAF and MEK Inhibitors Regulate the TumorProtein lysates were prepared in Laemmli sample buffer, separated by SDS-PAGE, and proteins were transferred to polyvinylidene dif-luoride membranes. Immunoreactivity was detected using HRP-con-jugated secondary antibodies (CalBioTech) and chemiluminescence substrate (Thermo Fisher Scientific) on a Versadoc Imaging Sys-tem (Bio-Rad). Primary antibodies, all from Cell Signaling Technol-ogy unless otherwise stated, were as follows: anti-phospho-ERK1/2 (T202/Y204), anti-ERK2 (Santa Cruz Biotechnology), anti-GSDME (Abcam), anti–cleaved caspase-3, anti-BIM/BOD (Enzo Life Sci-ences), anti-IL1α (Santa Cruz Biotechnology), anti-HMGB1, and anti-GAPDH. Cell supernatants were harvested in the absence of FBS in culture medium to avoid distortion of SDS-PAGE. After centrifu-gation to remove cell debris, cell supernatants were concentrated 10×using Amicon Ultra 10K (Sigma-Aldrich). Concentrates were mixed with Laemmli sample buffer (Bio-Rad) and analyzed via Western blotting. For T-cell culture, concentrates were further washed twice with PBS and once with RPMI-1640 medium by spin, and sterilized by filtration through a 0.2-μm filter. Protein gel staining was per-formed using Coomassie Brilliant Blue R-250.

Date published: 2020-10-27

Rated 3 out of 5 by jiapangpang1993 from need more testsI can not get a clear band in a 1:1000 dilution,I will change its concentration to see whether it can work or not

Date published: 2017-03-02

Rated 5 out of 5 by vagabond123 from IHC-PWe have detected positive immunofluorescence signal in human IHC-P sclides

Date published: 2016-12-12

Rated 3 out of 5 by HF from Good specificity in WBGood specificity in WB. -SCBT QC

Date published: 2013-08-16

IL-1α 抗体 (ALF-161) is rated 4.0 out of 5 by 4.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	IL1A	Entrez Gene ID: 3552	2q13	NM_000575	UniProt ID: P01583	147760
Mouse	Il1a	Entrez Gene ID: 16175	2 F1	XM_006498793, XM_006498794, NM_010554	UniProt ID: P01582	N/A

IL-1α 抗体 (ALF-161): sc-12741

IL-1α 抗体 (ALF-161) 规格

替代产品名称: IL-1α

产品克隆名称: ALF-161

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG

氨基酸:

抗原表位靶标: IL-1α

抗原表位种属: mouse

产品反应性/检测: IL-1α

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, FCM, ELISA

瓶的信息: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 8篇各种科学出版物的引文

優選的 IL-1α 抗體是 IL-1α 抗體 (G-10): sc-271618.