Date published: 2025-9-6

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IGFBP3 抗体 (E-9): sc-374365

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说明书
  • IGFBP3 抗体 E-9 是小鼠单克隆 IgG1 κ,IGFBP3抗体, 在10篇文献中引用,规格为200 µg/ml
  • 免疫human物种的IGFBP3的氨基酸113-210
  • IGFBP3 抗体 (E-9) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 precursor and mature IGFBP3
  • 抗IGFBP3抗体(E-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IGFBP3 (E-9): sc-374365免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是IGFBP3 Antibody (E-9) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与IGFBP3 Antibody (E-9) 打包提供(请参阅下面的订购信息)。

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関連項目

IGFBP3抗体(E-9)是一种IgG1 κ小鼠单克隆IGFBP3抗体(也称为IBP3抗体),可通过WB、IP、IF和ELISA检测人源的IGFBP3蛋白。IGFBP3抗体(E-9)既有非偶联形式的抗IGFBP3抗体,也有多种偶联形式的抗IGFBP3抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。胰岛素样生长因子结合蛋白(IGFBPs)是与IGFs共同进化的一类同源蛋白,它们不仅作为可溶性IGFs的穿梭分子,而且对IGF信号系统具有一定的调控作用。IGFBPs与IGF的物理结合影响生长因子的生物利用度以及它们在细胞外环境中的浓度和分布。IGFBPs似乎还具有不依赖于IGFs的生物学活性。目前已经描述了7种IGFBPs,它们在组织分布、半衰期以及调节IGF与其受体的相互作用方面各不相同。IGFBP1受到胰岛素产生的负调控。IGFBP1基因在胎儿肝脏发育过程中以及营养变化和糖尿病反应中高水平表达。IGFBP2可能作为伴侣蛋白,将IGFs护送到靶组织,它在包括胎儿眼睛和胎儿大脑在内的几个人体组织中表达。IGFBP3是最丰富的IGFBP,与大约80%的血清IGFs形成复合物。IGFBP3和IGFBP4均由皮肤成纤维细胞在切口损伤后释放。IGFBP5由肌细胞分泌,可能在肌肉分化中起关键作用。IGFBP6与其他IGFBPs的不同之处在于它对IGF-II的亲和力最高。糖基化的人IGFBP6在中国仓鼠卵巢(CHO)细胞中表达,而非糖基化的重组人IGFBP-6在大肠杆菌中表达。IGFBP7是一种分泌蛋白,它以相对较低的亲和力与IGF-I和IGF-II结合,刺激前列环素的产生,也可能作为生长抑制因子发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IGFBP3 抗体 (E-9) 参考文献:

  1. 调节激肽:物质P和神经激肽受体表达在眼表疾病中的作用。  |  Schott, M., et al. Lancet. 364: 325; author reply 326-7. PMID: 15276379
  2. Immunomodulation by tachykinin neuropeptides.  |  Naspi, A., et al. 2014. PLoS One. 9: e98641. PMID: 24905466
  3. IGFBP-3 在内脏脂肪组织中与肥胖相关的胰岛素信号转导中与维生素 D 受体相互作用  |  Moreno-Santos, I., et al. 2017. Int J Mol Sci. 18: PMID: 29112142
  4. Tachykinin NK2 antagonist for treatments of various disease states.  |  Li, N., et al. 2022. J Trace Elem Med Biol. 69: 126877. PMID: 34678598
  5. Mammalian tachykinin receptors.  |  Chen, S., et al. 2024. Environ Toxicol.. PMID: 38366283
  6. 胰岛素样生长因子结合蛋白作为葡萄糖调节剂。  |  Baxter, RC. 1995. Metabolism. 44: 12-7. PMID: 7476305
  7. IGFBP-1 基因的结构和定位及其在肝脏再生过程中的表达。  |  Lee, J., et al. 1994. Hepatology. 19: 656-65. PMID: 7509771
  8. 胰岛素样生长因子  |  Schmid, C. 1995. Cell Biol Int. 19: 445-57. PMID: 7640658
  9. 调节新陈代谢的 IGF 系统。  |  Binoux, M. 1995. Diabete Metab. 21: 330-7. PMID: 8586149
  10. 正常和增生性疤痕成纤维细胞 IGFBPs 的差异表达。  |  Hathaway, CL., et al. 1996. J Surg Res. 60: 156-62. PMID: 8592408
  11. 胰岛素样生长因子结合蛋白(IGFBPs)及其调控动态。  |  Kelley, KM., et al. 1996. Int J Biochem Cell Biol. 28: 619-37. PMID: 8673727
  12. 胰岛素样生长因子结合蛋白(IGFBP)-7 的合成与特性。重组人 mac25 蛋白可特异性结合 IGF-I 和 -II。  |  Oh, Y., et al. 1996. J Biol Chem. 271: 30322-5. PMID: 8939990

订购信息

产品名称产品编号规格价格数量收藏夹

IGFBP3 抗体 (E-9)

sc-374365
200 µg/ml
$316.00

IGFBP3 (E-9): m-IgG Fc BP-HRP 套装

sc-529729
200 µg Ab; 10 µg BP
$354.00

IGFBP3 (E-9): m-IgGκ BP-HRP 套装

sc-522712
200 µg Ab, 40 µg BP
$354.00

IGFBP3 抗体 (E-9) AC

sc-374365 AC
500 µg/ml, 25% agarose
$416.00

IGFBP3 抗体 (E-9) HRP

sc-374365 HRP
200 µg/ml
$316.00

IGFBP3 抗体 (E-9) FITC

sc-374365 FITC
200 µg/ml
$330.00

IGFBP3 抗体 (E-9) PE

sc-374365 PE
200 µg/ml
$343.00

IGFBP3 抗体 (E-9) Alexa Fluor® 488

sc-374365 AF488
200 µg/ml
$357.00

IGFBP3 抗体 (E-9) Alexa Fluor® 546

sc-374365 AF546
200 µg/ml
$357.00

IGFBP3 抗体 (E-9) Alexa Fluor® 594

sc-374365 AF594
200 µg/ml
$357.00

IGFBP3 抗体 (E-9) Alexa Fluor® 647

sc-374365 AF647
200 µg/ml
$357.00

IGFBP3 抗体 (E-9) Alexa Fluor® 680

sc-374365 AF680
200 µg/ml
$357.00

IGFBP3 抗体 (E-9) Alexa Fluor® 790

sc-374365 AF790
200 µg/ml
$357.00

There is one product citation (reference 1) included on your datasheet where IGFBP3 (E-9) has been used in P(IHC), but IHC is not listed in the applications for E-9. Is there a reason why it's not recommended?

Asked by: LinS
Thank you for your question. We recommend using a conjugated form for iHC. Anti-IGFBP3 Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM.
Answered by: BlakeJ
Date published: 2023-01-26

Can IGFBP3 (E-9): sc-374365 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IGFBP3 (E-9): sc-374365 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 效果很好一个月前领取的贵公司的一抗,western blo做出来效果很好,条带曝出来很清晰,无杂带。客服态度特别好
Date published: 2017-07-07
Rated 3 out of 5 by from WBThere were a 30kd and 55kd WB band detected in human macrophage samples, and I am not sure whether it works or not.
Date published: 2017-01-19
Rated 5 out of 5 by from Satisfactory Western Blot data of IGFBP3Satisfactory Western Blot data of IGFBP3 in human plasma. -SCBT QC
Date published: 2015-02-22
Rated 5 out of 5 by from Satisfactory Western Blot data of human recombinantSatisfactory Western Blot data of human recombinant IGFBP3. -SCBT QC
Date published: 2013-09-30
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IGFBP3 抗体 (E-9) is rated 4.5 out of 5 by 4.
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