Date published: 2025-9-7
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ICSBP Antibody (E-9) - Western Blotting - Image 355197
ICSBP 抗体 (E-9): sc-365042. M1 (A), BJAB (B) 和 NAMALWA (C) 全细胞裂解液中 ICSBP 表达的 Western 印迹分析.
ICSBP 抗体 (E-9): sc-365042. M1 (A), BJAB (B) 和 NAMALWA (C) 全细胞裂解液中 ICSBP 表达的 Western 印迹分析.

ICSBP 抗体 (E-9): sc-365042

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说明书
  • ICSBP 抗体 E-9 是小鼠单克隆 IgG2b κ，ICSBP抗体, 在24篇文献中引用，规格为200 µg/ml
  • 免疫human物种的ICSBP的氨基酸357-426
  • ICSBP 抗体 (E-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ICSBP
  • 抗ICSBP抗体（E-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-365042 X, 200 µg/0.1 ml)
  • 关于如何获取ICSBP (E-9): sc-365042的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 ICSBP 抗体 (E-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ICSBP 抗体 (E-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

ICSBP抗体（E-9）是一种IgG2b κ小鼠单克隆ICSBP抗体（也称为干扰素共识序列结合蛋白（ICSBP）抗体、ICSBP1抗体、干扰素调节因子8抗体、干扰素（IFN）调节因子（IRF）家族转录因子8抗体、IRF-8抗体、髓系细胞成熟相关转录因子抗体或髓系祖代单核细胞系介体抗体），它可以通过WB、IP、IF、IHC(P)和ELISA等方法检测小鼠、大鼠和人类的ICSBP蛋白。ICSBP抗体（E-9）既可以以非偶联的抗ICSBP抗体形式存在，也可以以多种偶联形式的抗ICSBP抗体存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。ICSBP（干扰素（IFN）共识序列结合蛋白，干扰素调节因子8；IRF-8）是一种转录因子，在树突状细胞和巨噬细胞分化过程中对IFN-γ介导的信号传导至关重要。ICSBP与TRAF6（在氨基酸残基356和305之间）发生物理相互作用，ICSBP与TRAF6的这种相互作用会调节TLR信号传导，并可能有助于IFN-γ和TLR信号通路之间的交叉对话。ICSBP通过下调Bcl-2来拮抗Bcr/Abl。已知ICSBP与染色质相互作用，并在巨噬细胞中与PU.1结合。ICSBP属于干扰素调节因子（IRF）家族，该家族还包括IRF-1、IRF-2和ISGF-3。这些蛋白质由位于N端区域的保守DNA结合域和不同的C端区域（充当调节域）组成。IRF家族蛋白与干扰素刺激反应元件（ISRE）结合，并调节IFN-α和IFN-β的表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ICSBP 抗体 (E-9) 参考文献:

  1. ICSBP 对小鼠 I 型干扰素产生细胞的发育以及 CD8alpha（+）树突状细胞的生成和活化至关重要。  |  Schiavoni, G., et al. 2002. J Exp Med. 196: 1415-25. PMID: 12461077
  2. ICSBP 在朗格汉斯细胞和真皮树突状细胞的正常发育和贩运过程中起着至关重要的作用。  |  Schiavoni, G., et al. 2004. Blood. 103: 2221-8. PMID: 14615368
  3. 干扰素共识序列结合蛋白（ICSBP；IRF-8）可拮抗 BCR/ABL 并下调 bcl-2。  |  Burchert, A., et al. 2004. Blood. 103: 3480-9. PMID: 14656881
  4. 干扰素调节因子ICSBP/IRF-8与PU.1结合可上调肿瘤抑制因子p15(Ink4b)在小鼠髓系细胞中的表达。  |  Schmidt, M., et al. 2004. Blood. 103: 4142-9. PMID: 14976051
  5. 分析 PU.1/ICSBP (IRF-8) 与各种 PU.1 突变体形成的复合物：大鼠 Icsbp (Irf-8) cDNA 的分子克隆。  |  Nakano, N., et al. 2005. Immunogenetics. 56: 871-7. PMID: 15688197
  6. IFN调节因子-4和-8控制树突状细胞亚群的发育及其功能多样性。  |  Tamura, T., et al. 2005. J Immunol. 174: 2573-81. PMID: 15728463
  7. 由 Cbl 介导的干扰素调节因子-8 的泛素依赖性降解可下调白细胞介素-12 的表达。  |  Xiong, H., et al. 2005. J Biol Chem. 280: 23531-9. PMID: 15837792
  8. 在活体巨噬细胞中可视化 IFN 调节因子 8 与染色质之间受伙伴调节的相互作用。  |  Laricchia-Robbio, L., et al. 2005. Proc Natl Acad Sci U S A. 102: 14368-73. PMID: 16183743
  9. IRF-8/干扰素（IFN）共识序列结合蛋白参与了Toll样受体（TLR）信号转导，并促成了TLR和IFN-gamma信号转导途径之间的交叉对话。  |  Zhao, J., et al. 2006. J Biol Chem. 281: 10073-80. PMID: 16484229
  10. ICSBP/IRF-8 在树突状细胞发育过程中对抗原摄取进行不同程度的调节，并影响 CD4+ T 细胞的抗原呈递。  |  Mattei, F., et al. 2006. Blood. 108: 609-17. PMID: 16569763
  11. 识别活化巨噬细胞中的 IRF-8 和 IRF-1 靶基因  |  Dror, N., et al. 2007. Mol Immunol. 44: 338-46. PMID: 16597464
  12. 干扰素调节因子 8 与干扰素调节因子 1, NF-kappaB 和 PU.1 合作调节 RANTES 基因转录。  |  Liu, J. and Ma, X. 2006. J Biol Chem. 281: 19188-95. PMID: 16707500

订购信息

产品名称产品编号规格价格数量收藏夹

ICSBP 抗体 (E-9)

sc-365042
200 µg/ml
$316.00

ICSBP (E-9): m-IgG Fc BP-HRP 套装

sc-529304
200 µg Ab; 10 µg BP
$354.00

ICSBP (E-9): m-IgGκ BP-HRP 套装

sc-522126
200 µg Ab, 40 µg BP
$354.00

ICSBP (E-9): m-IgG2b BP-HRP 套装

sc-550091
200 µg Ab; 10 µg BP
$354.00

ICSBP 抗体 (E-9) X

sc-365042 X
200 µg/0.1 ml
$316.00

ICSBP 抗体 (E-9) AC

sc-365042 AC
500 µg/ml, 25% agarose
$416.00

ICSBP 抗体 (E-9) HRP

sc-365042 HRP
200 µg/ml
$316.00

ICSBP 抗体 (E-9) FITC

sc-365042 FITC
200 µg/ml
$330.00

ICSBP 抗体 (E-9) PE

sc-365042 PE
200 µg/ml
$343.00

ICSBP 抗体 (E-9) Alexa Fluor® 488

sc-365042 AF488
200 µg/ml
$357.00

ICSBP 抗体 (E-9) Alexa Fluor® 546

sc-365042 AF546
200 µg/ml
$357.00

ICSBP 抗体 (E-9) Alexa Fluor® 594

sc-365042 AF594
200 µg/ml
$357.00

ICSBP 抗体 (E-9) Alexa Fluor® 647

sc-365042 AF647
200 µg/ml
$357.00

ICSBP 抗体 (E-9) Alexa Fluor® 680

sc-365042 AF680
200 µg/ml
$357.00

ICSBP 抗体 (E-9) Alexa Fluor® 790

sc-365042 AF790
200 µg/ml
$357.00

Is this antibody suitable for Flow Cytometry? If so, is there an appropriate Isotype control or peptide blocker? Thanks

Asked by: Kieran
Thank you for your question. We have not yet tested this antibody, ICSBP (E-9): sc-365042, for flow cytometry so we do not know if it is suitable for this application. This primary antibody is a mouse monoclonal IgG2b, and the corresponding unconjugated isotype control is normal mouse IgG2b: sc-3879 . This antibody is not raised against a peptide, so no blocking peptide is available. For a limited time we are offering a free 10µg sample of this antibody. If you would like to receive a sample and test it for flow cytometry, please contact our Technical Service Department. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-06-27

I am using ICSBP (E-9): sc-365042 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from It works wellIt works well in osteoclasts which are obtained from mouse.
Date published: 2021-07-21
Rated 4 out of 5 by from Good for Western Blot and IHCI used this antibody (IRF-8) for IHC staining of mouse eWAT (white adipose tissue) samples and worked very well however I couldnt optimize it for liver sample. WB analysis also nicely shows IRF8 band with some none specific bands.
Date published: 2020-12-19
Rated 5 out of 5 by from Works well for IP and WBI got a free sample a month ago and tested for both western blot and IP. It works very well for both.
Date published: 2018-01-27
Rated 5 out of 5 by from works very goodThis monoclonal antibody works very well for detection of IRF8 from human protein samples
Date published: 2017-06-12
Rated 5 out of 5 by from This monoclonal antibody works very wellThis monoclonal antibody works very well for detection of IRF8 from both mouse and human protein samples
Date published: 2015-11-02
Rated 5 out of 5 by from Robust signal in western blot analysis ofRobust signal in western blot analysis of ICSBP expression in IB4 and NAMALWA whole cell lysates. -SCBT QC
Date published: 2015-06-23
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-04-02
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ICSBP 抗体 (E-9) is rated 4.9 out of 5 by 7.
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