HSP 47 Antibody (E-4): sc-398579
- HSP 47 Antibody (E-4) is a mouse monoclonal IgG1 λ, cited in 1 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 18-36 at the N-terminus of HSP 47 of human origin
- recommended for detection of HSP 47 of mouse, rat and human origin by WB, IP, IF and ELISA
- m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for HSP 47 Antibody (E-4) for WB applications. This reagent is now offered in a bundle with HSP 47 Antibody (E-4) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
HSP 47 Antibody (E-4) is a mouse monoclonal IgG1 antibody that detects HSP 47 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HSP 47, also known as collagen 1, serpinh1, collagen-binding protein 1 (CBP1), and gp46, is a crucial molecular chaperone that plays a significant role in the biosynthesis of collagen type I. HSP 47 is predominantly located in the endoplasmic reticulum of cells that produce procollagen, where HSP 47 assists in the proper folding and assembly of collagen molecules, ensuring their structural integrity and functionality. HSP 47′s location is essential for the secretion of procollagen from these cells, thereby influencing the extracellular matrix formation and tissue repair processes. Additionally, HSP 47 acts as a stress response molecule, sequestering abnormal procollagen following cellular insult, which highlights HSP 47′s protective role in maintaining collagen homeostasis. HSP 47 synthesis is upregulated by cytokines such as transforming growth factor beta (TGFβ) and interleukin-1 beta (IL-1β), further emphasizing HSP 47′s involvement in cellular stress responses and tissue remodeling.
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HSP 47 Antibody (E-4) References:
- Hsp47 a novel collagen binding serpin chaperone, autoantigen and therapeutic target. | Sauk, JJ., et al. 2005. Front Biosci. 10: 107-18. PMID: 15574354
- Ancestry & molecular evolutionary analyses of heat shock protein 47 kDa (HSP47/SERPINH1). | Kumar, A., et al. 2017. Sci Rep. 7: 10394. PMID: 28871169
- Mutational hotspots of HSP47 and its potential role in cancer and bone-disorders. | Parveen, A., et al. 2020. Genomics. 112: 552-566. PMID: 30986427
- Cell-specific ablation of Hsp47 defines the collagen-producing cells in the injured heart. | Khalil, H., et al. 2019. JCI Insight. 4: e128722. PMID: 31393098
- Heat shock protein 47 (HSP47) binds to discoidin domain-containing receptor 2 (DDR2) and regulates its protein stability. | Chen, J., et al. 2019. J Biol Chem. 294: 16846-16854. PMID: 31570520
- Mechanisms of procollagen and HSP47 sorting during ER-to-Golgi trafficking. | Omari, S., et al. 2020. Matrix Biol. 93: 79-94. PMID: 32562852
- New specific HSP47 functions in collagen subfamily chaperoning. | Köhler, A., et al. 2020. FASEB J. 34: 12040-12052. PMID: 32716577
- Profibrotic COVID-19 subphenotype exhibits enhanced localized ER-dependent HSP47+ expression in cardiac myofibroblasts in situ. | Jacobs, ER., et al. 2023. J Mol Cell Cardiol. 185: 1-12. PMID: 37839656
- Levels of Small Extracellular Vesicles Containing hERG-1 and Hsp47 as Potential Biomarkers for Cardiovascular Diseases. | Osorio, LA., et al. 2024. Int J Mol Sci. 25: PMID: 38732154
- Regulation and function of collagen-specific molecular chaperone, HSP47. | Nagata, K. and Hosokawa, N. 1996. Cell Struct Funct. 21: 425-30. PMID: 9118251
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 47 Antibody (E-4) | sc-398579 | 200 µg/ml | $322.00 | |||
HSP 47 Antibody (E-4): m-IgGλ BP-HRP Bundle | sc-524400 | 200 µg Ab, 40 µg BP | $361.00 | |||
HSP 47 (E-4) Neutralizing Peptide | sc-398579 P | 100 µg/0.5 ml | $69.00 |