Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSP 40 CRISPR/Cas9 KO Plasmid (h): sc-402810
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSP 40 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HSP 40 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSP 40 Antibody (B-3): sc-398766
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 40 CRISPR/Cas9 KO Plasmid (h) | sc-402810 | 20 µg | $397.00 |
Overview
DNAJB1 encodes the human HSP40 co-chaperone (DnaJ homolog subfamily B member 1), a key regulator of proteostasis that stimulates HSP70 ATPase activity to promote folding, refolding, and triage of misfolded client proteins. Through its J-domain–dependent interaction with HSP70, HSP40 supports cytosolic protein quality control, stress responses, and coordination of chaperone-assisted degradation via proteasome and autophagy-linked pathways. DNAJB1 function is relevant to cellular resilience during heat shock and other proteotoxic stresses, and altered chaperone networks are frequently implicated in protein aggregation disorders and oncogenic stress adaptation. A recurrent disease-associated event involving DNAJB1 is the DNAJB1–PRKACA fusion observed in fibrolamellar carcinoma, highlighting the value of DNAJB1-focused models for studying chaperone biology in defined genetic contexts.
HSP 40 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the DNAJB1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the DNAJB1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the DNAJB1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HSP 40 protein expression.
This CRISPR knockout system enables efficient generation of DNAJB1-deficient cell models for investigation of HSP 40 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting DNAJB1 exon(s) critical for HSP 40 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple DNAJB1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HSP 40 CRISPR/Cas9 KO Plasmid (h) and HSP 40 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the DNAJB1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HSP 40 HDR Plasmid (h) and HSP 40 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by DNAJB1 homology arms to support homology-directed repair at defined DNAJB1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.