Date published: 2025-9-7
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HMG-1/HMGB1 Antibody (HAP46.5) - Western Blotting - Image 368033
HMG-1 抗体 (HAP46. 5): sc-56698. HeLa (A), Jurkat (B), MCF7 (C), NIH/3T3 (D) 和 PC-12 (E) 全细胞裂解液中 HMG-1 表达的 Western 印迹分析.
HMG-1 抗体 (HAP46. 5): sc-56698. HeLa (A), Jurkat (B), MCF7 (C), NIH/3T3 (D) 和 PC-12 (E) 全细胞裂解液中 HMG-1 表达的 Western 印迹分析.

HMG-1/HMGB1 抗体 (HAP46.5): sc-56698

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寫評論提問

说明书
  • HMG-1/HMGB1 抗体 HAP46.5 是小鼠单克隆 IgG1 κ，HMG-1/HMGB1抗体, 在59篇文献中引用，规格为50 µg/0.5 ml
  • 针对全长重组HMG-1，human起源
  • 推荐用于 mouse, rat， human 和 Arabadopsis 来源的HMG-1 WB, IP 和 ELISA检测; 也和以下物种反应，包括： canine
  • 关于如何获取HMG-1/HMGB1 (HAP46.5): sc-56698的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是HMG-1/HMGB1 Antibody (HAP46.5) for WB applications. 的首选辅助检测试剂，这些试剂现与HMG-1/HMGB1 Antibody (HAP46.5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加HMG抗体，包括HMG-1, HMG-14, HMG-2, HMG-20A, HMG-2L1, HMG-I, HMG-Y和HMGI-C

HMG-1 抗体（HAP46.5）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过西部印迹（WB）、免疫沉淀（IP）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人源的 HMG-1 蛋白。抗 HMG-1 抗体 (HAP46.5) 以非结合形式提供。包括 HMG-1 在内的高迁移率基团（HMG）蛋白在基因表达和染色质结构的调控中起着至关重要的作用。具体来说，HMG-1 与 Oct-2 和 NFκB 家族成员等各种转录因子相互作用，增强它们激活转录的能力。这种相互作用对于细胞对压力和炎症的反应至关重要，因为 HMG-1 可以作为一种信号分子影响免疫反应。HMG-1 主要位于细胞核中，与 DNA 结合后会引起结构变化，从而促进转录机制与特定基因的连接。HMG-1 有两个 DNA 结合域（称为 HMG 盒），可与单链和双链 DNA 结合，但主要诱导双链 DNA 发生构象变化。了解 HMG-1 的功能和相互作用对于阐明其在发育、分化和细胞对损伤的反应等各种生物过程中的作用至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HMG-1/HMGB1 抗体 (HAP46.5) 参考文献:

  1. HMG-1是小鼠内毒素致死性的晚期介质。  |  Wang, H., et al. 1999. Science. 285: 248-51. PMID: 10398600
  2. 高迁移率组蛋白1（HMG-1）刺激人类单核细胞中促炎细胞因子的合成。  |  Andersson, U., et al. 2000. J Exp Med. 192: 565-70. PMID: 10952726
  3. HMG-1被重新发现是一种细胞因子。  |  Yang, H., et al. 2001. Shock. 15: 247-53. PMID: 11303722
  4. 人类 HMG2 蛋白编码基因的结构。  |  Shirakawa, H. and Yoshida, M. 1992. J Biol Chem. 267: 6641-5. PMID: 1551873
  5. HMG 染色体蛋白及其基因的结构特征。  |  Bustin, M., et al. 1990. Biochim Biophys Acta. 1049: 231-43. PMID: 2200521
  6. 编码非组蛋白染色体蛋白 HMG-1 的人类胎盘 cDNA 克隆。  |  Wen, L., et al. 1989. Nucleic Acids Res. 17: 1197-214. PMID: 2922262
  7. 三唑类抗真菌药在曲霉菌中的次要作用机制由hmg1介导。  |  Rybak, JM., et al. 2024. Nat Commun. 15: 3642. PMID: 38684680
  8. 除 NF-kappa B 外，HMGI(Y) 和 Sp1 还调控 MGSA/GRO alpha 基因的转录。  |  Wood, LD., et al. 1995. Nucleic Acids Res. 23: 4210-9. PMID: 7479086
  9. 雷帕霉素可抑制白细胞介素 4 诱导的 HMG-I（Y）磷酸化。  |  Wang, DZ., et al. 1995. J Biol Chem. 270: 22924-32. PMID: 7559428
  10. 建筑转录因子 LEF-1 弯曲 DNA 的结构基础  |  Love, JJ., et al. 1995. Nature. 376: 791-5. PMID: 7651541
  11. 通过与高迁移率基团蛋白 I/Y 结合，封闭的环状 DNA 中出现了超重性变化。  |  Nissen, MS. and Reeves, R. 1995. J Biol Chem. 270: 4355-60. PMID: 7876198
  12. 转录因子和结构蛋白 HMG I(Y)可逆转 IFN beta 基因增强子中固有的 DNA 弯曲。  |  Falvo, JV., et al. 1995. Cell. 83: 1101-11. PMID: 8548798

订购信息

产品名称产品编号规格价格数量收藏夹

HMG-1/HMGB1 抗体 (HAP46.5)

sc-56698
50 µg/0.5 ml
$316.00

HMG-1/HMGB1 (HAP46.5): m-IgG Fc BP-HRP 套装

sc-539036
50 µg Ab; 10 µg BP
$354.00

HMG-1/HMGB1 (HAP46.5): m-IgG1 BP-HRP 套装

sc-541219
50 µg Ab; 20 µg BP
$354.00

Hi, I would like to order an Hmgb1 antibody for immunoprecipitation from human macrophage cells. Which one is the best? Sc-56698, Sc-130024, Sc-393213? Thanks!

Asked by: gulg_genetic2
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-11-02

能否应用于coip有相关文献支持嘛？

Asked by: Zqq414666
感谢您的提问。HMG-1/HMGB1 (HAP46.5): sc-56698 还没有经过CoIP实验的验证，因此，目前不推荐应用于CoIP. Thank you for your inquiry. We have not yet tested HMG-1/HMGB1 (HAP46.5): sc-56698 on CoIP. As a result, CoIP is not currently a recommended application for this antibody.
Answered by: Sen Li
Date published: 2021-07-14

Does this antibody works well in Co-Immunoprecipitation experiments? What about immunocytochemistry?

Asked by: Dana I
Thank you for your question. We have not tested the HMG-1 Antibody (HAP46.5): sc-56698 for co-immunoprecipitation experiments, nor are we aware of anyone citing the antibody for this research application. The antibody is recommended and warrantied for immunoprecipitation of the HMG-1 antigen. We do not currently recommend this antibody for immunocytochemistry (or IF). However, there is a publication citing use of the antibody for this research application, see PMID: 24091659.
Answered by: Technical Support
Date published: 2017-02-06

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using withHMG-1 (HAP46.5): sc-56698?

Asked by: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-13

Is this antibody reactive with avian samples?

Asked by: TinTin
Yes, this antibody is expected to pick up avian HMG-1. However, we recomment to contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2017-01-13
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Rated 4 out of 5 by from The antibody works well for western blotThe sc-56698 antibody detects hmg1 protein in the supernatant of rotavirus infected cells. The monomeric band weights 29 KDa.
Date published: 2024-02-20
Rated 5 out of 5 by from Very goodThis antibody is good in both sensitivity and specificity for detecting HMGB1.
Date published: 2021-08-19
Rated 5 out of 5 by from Great antibodyGreat antibody for WB. Used on PVDF membrane. Hela lysate and dilution 1/100 in blocking buffer 3%BSA 2% milk o/n 4C.
Date published: 2019-08-23
Rated 4 out of 5 by from GreatMy lab has purchased this antibody many times and we are very pleased
Date published: 2018-09-04
Rated 4 out of 5 by from Not very well.I couldn't get the predicted band at the dilution of 1:1000 in western blot,Maybe I should try again.
Date published: 2018-04-04
Rated 3 out of 5 by from works for WBI used this antibody for WB to test the expression of HMG-1 in 293T cells, the dilution ratio was 1:500. It works, but the bind was faint. I suggest reducing dilution ratio.
Date published: 2018-03-17
Rated 5 out of 5 by from good on WBI try this antibody on primary microglia and neuron to do Western Blot, the dilution is 1:400, It worked very good.
Date published: 2017-09-11
Rated 5 out of 5 by from ExcellentSingle band with out any background, excellent result
Date published: 2017-09-07
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HMG-1/HMGB1 抗体 (HAP46.5) is rated 4.7 out of 5 by 18.
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