Date published: 2025-9-8

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His-Tag 抗体 (AD1.1.10): sc-53073

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说明书
  • His-Tag抗体(AD1.1.10)是小鼠单克隆IgG1 κ, 在66篇文献中引用,规格为200 µg/ml
  • 针对6x His标签的多肽
  • His-Tag 抗体 (AD1.1.10) 推荐用于 WB, IP 和 IF,检测 fusion proteins encoded by polyhistidine expression vectors
  • 抗His-Tag抗体(AD1.1.10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取His-Tag (AD1.1.10): sc-53073免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是His-Tag Antibody (AD1.1.10) for WB applications. 的首选辅助检测试剂,这些试剂现与His-Tag Antibody (AD1.1.10) 打包提供(请参阅下面的订购信息)。

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関連項目

His-Tag抗体(AD1.1.10)是一种小鼠单克隆IgG1 kappa轻链抗体,针对6x His标签的多肽产生,设计用于诸如蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)等应用。His标签是通过基因工程技术在目标蛋白的N端或C端添加的六组组氨酸残基的短序列。该标签至关重要,因为它有助于纯化和检测重组蛋白。组氨酸残基对镍和钴等金属离子具有极强的亲和力,使标记的蛋白能够在亲和色谱法中有效地与金属螯合树脂结合。这样就可以一步完成纯化过程,不仅高效,而且能够保持蛋白质的天然结构和功能。His标签体积小,对蛋白质活性的干扰最小,是蛋白质研究的理想选择。对于从事蛋白质表达、结构功能分析和治疗药物开发的研究人员来说,理解并利用His标签非常重要,因为有了它,研究人员可以轻松分离和鉴定原本难以研究的蛋白质。抗His-Tag抗体(AD1.1.10)可特异性结合His-Tag序列,使研究人员能够在各种实验应用中检测和分析His-Tag蛋白,从而推进分子生物学、生物化学及相关领域的研究。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

His-Tag 抗体 (AD1.1.10)

sc-53073
200 µg/ml
$316.00

His-Tag (AD1.1.10): m-IgG Fc BP-HRP 套装

sc-526762
200 µg Ab; 10 µg BP
$354.00

His-Tag (AD1.1.10): m-IgG1 BP-HRP 套装

sc-532135
200 µg Ab; 20 µg BP
$354.00

His-probe 抗体 (AD1.1.10) AC

sc-53073 AC
500 µg/ml, 25% agarose
$416.00

His-probe 抗体 (AD1.1.10) HRP

sc-53073 HRP
200 µg/ml
$316.00

His-probe 抗体 (AD1.1.10) FITC

sc-53073 FITC
200 µg/ml
$330.00

His-probe 抗体 (AD1.1.10) PE

sc-53073 PE
200 µg/ml
$343.00

His-probe 抗体 (AD1.1.10) Alexa Fluor® 488

sc-53073 AF488
200 µg/ml
$357.00

His-probe 抗体 (AD1.1.10) Alexa Fluor® 546

sc-53073 AF546
200 µg/ml
$357.00

His-probe 抗体 (AD1.1.10) Alexa Fluor® 594

sc-53073 AF594
200 µg/ml
$357.00

His-probe 抗体 (AD1.1.10) Alexa Fluor® 647

sc-53073 AF647
200 µg/ml
$357.00

His-probe 抗体 (AD1.1.10) Alexa Fluor® 680

sc-53073 AF680
200 µg/ml
$357.00

His-probe 抗体 (AD1.1.10) Alexa Fluor® 790

sc-53073 AF790
200 µg/ml
$357.00

Can this antibody detect the His-tag on the N- and/or C-terminus?

Asked by: Nick
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical Service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-08-06

Does this antibody detect the His-tag on both the N- and the C-terminus?

Asked by: Nick
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical Service. Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-08-06

This antibody can detect His tagged at the middle of the protein construct by Western Blot (while many products can't). 

Asked by: Pattama
Thank you for your question. Please contact our Technical Service Department by phone, e-mail, or live chat. We are happy to help you with any questions regarding this antibody.
Answered by: SCBT Representative
Date published: 2022-03-09

If i use the HIS antibody for an IP to pull-down a HIS-Tagged protein, could imidazole be used to elute the HIS tag protein from the HIS antibody ?

Asked by: j1234t1234
Thank you for your question. We have not tested the antibody using this type of elution buffer. Please refer to the product citations on our website using this antibody for IP experiments, or contact us for further Technical Support.
Answered by: Technical Support
Date published: 2023-04-23

Is His-probe Antibody (AD1.1.10): sc-53073 available to useful to FACS?

Asked by: piliya
Thank you for your question. We have not tested this antibody, His-probe (AD1.1.10): sc-53073, for FACS or FCM and so these are not recommended applications at this time.
Answered by: Technical Support
Date published: 2023-04-23

Is His-probe Antibody (AD1.1.10): sc-53073 available to useful to ELISA? If not, please recommend.

Asked by: George C
Thank you for your question. We are aware of one publication citing use of this antibody, His-probe (AD1.1.10): sc-53073, for use in an ELISA application. Please refer to PMID number 25174000 on PubMed for this citation. As an alternative, we would recommend using our mouse monoclonal antibody, His-probe (H-3): sc-8036, which is very popular and has been cited in over 10 publications for use in ELISA applications. https://www.scbt.com/scbt/product/his-probe-antibody-h-3
Answered by: Technical Support
Date published: 2023-04-23

Is His-probe Antibody (AD1.1.10): sc-53073 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using His-probe (H-3), which is available conjugated to agarose, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. (https://www.scbt.com/scbt/product/his-probe-antibody-h-3)
Answered by: Technical Support 8
Date published: 2023-11-25
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Rated 5 out of 5 by from The antibody is great!The antibody works very well and is highly recommended, I trust santa cruz!
Date published: 2025-05-27
Rated 5 out of 5 by from Excellent!I bought this product. It's very easy to use. I recommend you to buy this product.
Date published: 2024-01-11
Rated 5 out of 5 by from 功能很出色实验室一直都是用的santa的各种抗体,总的来说效果还不错 实验室一直都是用的santa的各种抗体,总的来说效果还不错
Date published: 2023-09-12
Rated 5 out of 5 by from 功能很出色实验室用的都是santa的抗体,总的来说效果还不错,还可以,实验室用的都是santa的抗体,总的来说效果还不错,还可以
Date published: 2023-09-12
Rated 5 out of 5 by from GhhhOke. Good. Skkwmmekdmmsksmsksksoksjsjsjsjsbhwuwhwhhsh
Date published: 2023-08-09
Rated 4 out of 5 by from YesPhotoshop verry good thank you app Verry,verry good
Date published: 2023-07-28
Rated 4 out of 5 by from Good antibody for ELISAAntibody works well in ELISA to detect His-tagged proteins. Low or no background and detection of native and denatured proteins possible.
Date published: 2023-01-27
Rated 5 out of 5 by from Work well for Western BlotThis antibody works well to detect HIS tagged at the middle of the protein construct (while some antibodies from other makers can't).
Date published: 2022-03-08
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His-Tag 抗体 (AD1.1.10) is rated 4.8 out of 5 by 23.
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