Date published: 2025-9-8

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Hephaestin 抗体 (C-7): sc-365365

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说明书
  • Hephaestin 抗体 C-7 是小鼠单克隆 IgG2b κ,Hephaestin抗体, 在8篇文献中引用,规格为200 µg/ml
  • 针对Hephaestin的human起源的N-terminal细胞外域内的氨基酸331-420
  • Hephaestin 抗体 (C-7) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Hephaestin
  • 抗Hephaestin抗体(C-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Hephaestin (C-7): sc-365365免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是Hephaestin Antibody (C-7) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Hephaestin Antibody (C-7) 打包提供(请参阅下面的订购信息)。

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関連項目

Hephaestin抗体(C-7)是一种IgG2b κ小鼠单克隆Hephaestin抗体(也称为Hephaestin抗体),可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学(IHC(P))和酶联免疫吸附测定(ELISA)检测人类来源的Hephaestin蛋白。Hephaestin抗体(C-7)既有非偶联形式的抗Hephaestin抗体,也有多种偶联形式的抗Hephaestin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Hephaestin是一种单次跨膜I型膜蛋白,属于多铜氧化酶家族蛋白。Hephaestin是一种铜依赖性铁氧化酶蛋白,对于铁从肠道肠细胞进入循环至关重要。它与铁转运蛋白1一起介导铁穿过基底外侧膜的运动。这是哺乳动物系统中铁和铜代谢之间的重要联系,因为铜缺乏会导致Hephaestin减少和铁吸收减少,从而导致贫血。每个Hephaestin单体可以结合六个铜离子,并受同源盒转录因子CDX2的调控。铁水平的升高会导致CDX2表达增加,进而Hephaestin增加。Hephaestin主要在肠道中检测到,但也在结肠、乳房、骨小梁细胞和成纤维细胞中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Hephaestin 抗体 (C-7) 参考文献:

  1. 膳食缺铜会降低雄性和雌性大鼠的铁吸收率和十二指肠肠细胞α蛋白。  |  Reeves, PG., et al. 2005. J Nutr. 135: 92-8. PMID: 15623839
  2. 肝素--细胞铁输出的铁氧化酶  |  Petrak, J. and Vyoral, D. 2005. Int J Biochem Cell Biol. 37: 1173-8. PMID: 15778082
  3. CDX2调节肠道和结肠上皮细胞中铁转运蛋白hephaestin的表达。  |  Hinoi, T., et al. 2005. Gastroenterology. 128: 946-61. PMID: 15825077
  4. 给缺铜大鼠补充膳食铜可恢复十二指肠α蛋白和铁的吸收。  |  Reeves, PG. and Demars, LC. 2005. Exp Biol Med (Maywood). 230: 320-5. PMID: 15855298
  5. 十二指肠 Dcytb 和 hephaestin mRNA 的表达不受体内铁平衡变化的显著影响。  |  Gleeson, F., et al. 2005. Blood Cells Mol Dis. 35: 303-8. PMID: 16137899
  6. 血铁和非血铁的吸收机制:从遗传性铁代谢紊乱中汲取的教训。  |  Anderson, GJ., et al. 2005. Biometals. 18: 339-48. PMID: 16158226
  7. 肠道铁运输的最新进展。  |  Anderson, GJ. and Frazer, DM. 2005. Curr Gastroenterol Rep. 7: 365-72. PMID: 16168234

订购信息

产品名称产品编号规格价格数量收藏夹

Hephaestin 抗体 (C-7)

sc-365365
200 µg/ml
$316.00

Hephaestin (C-7): m-IgGκ BP-HRP 套装

sc-522223
200 µg Ab, 40 µg BP
$354.00

Hephaestin (C-7): m-IgG2b BP-HRP 套装

sc-549015
200 µg Ab; 10 µg BP
$354.00

Hephaestin 抗体 (C-7) AC

sc-365365 AC
500 µg/ml, 25% agarose
$416.00

Hephaestin 抗体 (C-7) HRP

sc-365365 HRP
200 µg/ml
$316.00

Hephaestin 抗体 (C-7) FITC

sc-365365 FITC
200 µg/ml
$330.00

Hephaestin 抗体 (C-7) PE

sc-365365 PE
200 µg/ml
$343.00

Hephaestin 抗体 (C-7) Alexa Fluor® 488

sc-365365 AF488
200 µg/ml
$357.00

Hephaestin 抗体 (C-7) Alexa Fluor® 546

sc-365365 AF546
200 µg/ml
$357.00

Hephaestin 抗体 (C-7) Alexa Fluor® 594

sc-365365 AF594
200 µg/ml
$357.00

Hephaestin 抗体 (C-7) Alexa Fluor® 647

sc-365365 AF647
200 µg/ml
$357.00

Hephaestin 抗体 (C-7) Alexa Fluor® 680

sc-365365 AF680
200 µg/ml
$357.00

Hephaestin 抗体 (C-7) Alexa Fluor® 790

sc-365365 AF790
200 µg/ml
$357.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Hephaestin (C-7): sc-365365?

Asked by: TinTin
We recommend using one of our our exclusive Mouse IgG Binding Proteins conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Best antibody for WBUsed on human cells and mouse tissue and works great
Date published: 2022-04-05
Rated 5 out of 5 by from Ottimo funzionamento in immunoistochimicaHo acquistato questo articolo che ha dato un ottimo segnale specifico su tessuto di duodeno (controllo di espressione)
Date published: 2020-03-09
Rated 1 out of 5 by from IF in Heph KO vs Heph+/=We tested this antibody in Heph-/- and Heph+/+ retina and brain tissue. IF stain in +/+ tissue appeared non-specific. The staining pattern of the +/+ and -/- tissue was identical.
Date published: 2017-02-11
Rated 5 out of 5 by from Produced nice Western blot data of HephaestinProduced nice Western blot data of Hephaestin expression in K-562 whole cell lysate. -SCBT QC
Date published: 2013-04-12
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Hephaestin 抗体 (C-7) is rated 4.0 out of 5 by 4.
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