Date published: 2025-9-7
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Hemoglobin α Antibody (D-4) - Western Blotting - Image 372310
血红蛋白 α 抗体 (D-4) HRP: sc-514378 HRP. K-562 (A) 和 HEL 92. 1. 7 (B) 全细胞裂解液及人血浆 (C) 中血红蛋白 α 表达的直接 Western 印迹分析.
血红蛋白 α 抗体 (D-4) HRP: sc-514378 HRP. K-562 (A) 和 HEL 92. 1. 7 (B) 全细胞裂解液及人血浆 (C) 中血红蛋白 α 表达的直接 Western 印迹分析.

Hemoglobin α 抗体 (D-4): sc-514378

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说明书
  • Hemoglobin alpha 抗体 D-4 是小鼠单克隆 IgG1 κ，Hemoglobin α抗体, 在15篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Hemoglobin α的氨基酸62-142
  • Hemoglobin alpha 抗体 (D-4) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Hemoglobin α
  • 抗Hemoglobin alpha抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Hemoglobin α (D-4): sc-514378的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Hemoglobin α Antibody (D-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Hemoglobin α Antibody (D-4) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Hemoglobin抗体，包括Hemoglobin α, Hemoglobin β, Hemoglobin δ, Hemoglobin ε, Hemoglobin γ和Hemoglobin ζ

血红蛋白α抗体（D-4）是一种IgG1 κ小鼠单克隆血红蛋白α抗体（也称为血红蛋白亚基α抗体、血红蛋白四聚体α链抗体、α珠蛋白抗体、α-珠蛋白抗体、血红蛋白α抗体或血红蛋白亚基α抗体），可通过WB、IP、IF和ELISA检测人源的血红蛋白α蛋白。血红蛋白α抗体（D-4）有非偶联的抗血红蛋白α抗体形式，以及多种偶联形式的抗血红蛋白α抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。血红蛋白（Hgb）与四个铁结合、亚甲基连接的四吡咯环（血红素）偶联。α（16p13.3；5'-ζ-pseudoz-pseudo α2-pseudo α1-α2-α1-θ1-3'）和β（11p15.5）珠蛋白基因座决定了基本的血红蛋白结构。血红蛋白的珠蛋白部分由两条α链和两条β链组成，它们成对排列形成四聚体。这四个珠蛋白链中的每一个都与一个血红素基团共价结合。α链和β链之间的键比相似珠蛋白链之间的键弱，从而形成一个对氧结合和释放重要的裂解平面。当α1-β2裂解平面松弛时，对氧的亲和力高。当两个α1-β2界面紧密结合时，血红蛋白对氧的亲和力低。含有两条α链加两条β链的Hb A占循环血红蛋白总量的97%。剩余3%的循环血红蛋白由Hb A-2（由两条α链加两条δ链组成）和胎儿血红蛋白（Hb F，由两条α链和两条γ链组成）组成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Hemoglobin α 抗体 (D-4) 参考文献:

  1. 在无细胞系统中表达的人类血红蛋白（Hb）β-和γ-球蛋白链与α-球蛋白链组装成 Hb A 和 Hb F。  |  Adachi, K., et al. 2002. J Biol Chem. 277: 13415-20. PMID: 11827978
  2. 镰状血红蛋白纤维组装中相互作用的联系：α-链 AB 区突变产生的抑制作用被其 EF 角的突变所抵消。  |  Sudha, R., et al. 2004. J Biol Chem. 279: 20018-27. PMID: 14982923
  3. 人类α-血红蛋白通过其伴侣蛋白转移到β-血红蛋白：一种新状态的证据。  |  Baudin-Creuza, V., et al. 2004. J Biol Chem. 279: 36530-3. PMID: 15220346
  4. AHSP 介导稳定α-血红蛋白的分子机制。  |  Feng, L., et al. 2004. Cell. 119: 629-40. PMID: 15550245
  5. 血管壁中的血红蛋白 α。  |  Butcher, JT., et al. 2014. Free Radic Biol Med. 73: 136-42. PMID: 24832680
  6. 基于生物信息学分析的α和β血红蛋白在非小细胞肺癌中的作用。  |  Kang, N., et al. 2022. Mol Carcinog. 61: 587-602. PMID: 35394695
  7. 血红蛋白α链变异体扎拉与家族性无症状低氧血症有关。  |  Pasquarella, A., et al. 2022. J Hematol. 11: 190-195. PMID: 36406831
  8. 血红蛋白α的C终止子32-mer片段是一种具有抗菌特性的淀粉样肽。  |  Olari, LR., et al. 2023. Cell Mol Life Sci. 80: 151. PMID: 37198527
  9. 血红蛋白α是T淋巴细胞中对氧化还原反应敏感的线粒体相关蛋白。  |  Reed, EC., et al. 2024. Free Radic Biol Med. 227: 1-11. PMID: 39586383
  10. 人类 zeta-globin 基因复合物长度多态性的分子基础。  |  Goodbourn, SE., et al. 1983. Proc Natl Acad Sci U S A. 80: 5022-6. PMID: 6308667
  11. 人类α-球蛋白α1和α2基因座的同源性和协同进化。  |  Liebhaber, SA., et al. 1981. Nature. 290: 26-9. PMID: 7010180
  12. 血红蛋白的多重功能  |  Giardina, B., et al. 1995. Crit Rev Biochem Mol Biol. 30: 165-96. PMID: 7555018

订购信息

产品名称产品编号规格价格数量收藏夹

Hemoglobin α 抗体 (D-4)

sc-514378
200 µg/ml
$316.00

Hemoglobin α (D-4): m-IgG Fc BP-HRP 套装

sc-531102
200 µg Ab; 10 µg BP
$354.00

Hemoglobin α (D-4): m-IgGκ BP-HRP 套装

sc-524700
200 µg Ab, 40 µg BP
$354.00

Hemoglobin α (D-4): m-IgG1 BP-HRP 套装

sc-544397
200 µg Ab; 20 µg BP
$354.00

Hemoglobin α 抗体 (D-4) AC

sc-514378 AC
500 µg/ml, 25% agarose
$416.00

Hemoglobin α 抗体 (D-4) HRP

sc-514378 HRP
200 µg/ml
$316.00

Hemoglobin α 抗体 (D-4) FITC

sc-514378 FITC
200 µg/ml
$330.00

Hemoglobin α 抗体 (D-4) PE

sc-514378 PE
200 µg/ml
$343.00

Hemoglobin α 抗体 (D-4) Alexa Fluor® 488

sc-514378 AF488
200 µg/ml
$357.00

Hemoglobin α 抗体 (D-4) Alexa Fluor® 546

sc-514378 AF546
200 µg/ml
$357.00

Hemoglobin α 抗体 (D-4) Alexa Fluor® 594

sc-514378 AF594
200 µg/ml
$357.00

Hemoglobin α 抗体 (D-4) Alexa Fluor® 647

sc-514378 AF647
200 µg/ml
$357.00

Hemoglobin α 抗体 (D-4) Alexa Fluor® 680

sc-514378 AF680
200 µg/ml
$357.00

Hemoglobin α 抗体 (D-4) Alexa Fluor® 790

sc-514378 AF790
200 µg/ml
$357.00

What Is the Manufacturing Expiry date for this item? Can I get a COA for the same?

Asked by: Rishabh
Thank you for your question. To request a manufacture date please email the lot number to scbt@scbt.com . We do not provide an expiration date but we do have a 1 year warranty from date of purchase. We do not provide a COA or SDS for our antibodies since they are non- hazardous. Here is the data sheet : https://datasheets.scbt.com/sc-514378.pdf
Answered by: BlakeJ
Date published: 2024-08-06

Would like to know if there is any data regarding how does this antibody works with mouse-origin HBA and what kind of dilution could be suggested when using it to detect low abundant HBA (non-erythroid origin cells)

Asked by: LuísMonteiro
Thank you for your question. We do not recommend Hemoglobin α Antibody (D-4): sc-514378 for the detection of Hemoglobin α in murine samples. However, there is a publication that successfully used this antibody with murine samples in western blotting (PMID 29500821). The recommended starting dilution for human samples is 1:100 with a dilution range of 1:100-1:1000. For further information please contact technical service.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I want to run a Western blot using this Hemoglobin alpha monoclonal antibody using K-562 whole cell lysate. How much should I load on my gel?

Asked by: TinTin
We recommend loading ~50µg of cell lysate on your gel. You can find more about the recommended conditions in our Western blotting protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 4
Date published: 2017-02-08
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Rated 5 out of 5 by from very specificreally very good. No non-spec and very strong signal even with HSC-derived cells, not just with RBC
Date published: 2022-12-12
Rated 4 out of 5 by from Nice antibodyUsed with 20 ug of protein extract from erythroid precursors and worked nicely at 1:200 dilution. Nice specific bands.
Date published: 2021-04-22
Rated 5 out of 5 by from Worked wellWe used this antibody for WB of K562 cells. Loaded 20 ug protein, used primary Ab at 1/200 dilution, secondary at 1/1000 (anti-mouse-AP). Observed chemiluminescence band with lots of background (Immun-Star substrate), and a very clean band, no background using chromogenic substrate (BCIP/NBT).
Date published: 2018-08-22
Rated 4 out of 5 by from workedtwo month ago, i applied for this antibody and used it to detect the protein in Red blood cell sample, in generally, it worked, but instability.
Date published: 2017-03-04
Rated 5 out of 5 by from Good results in WBThis monoclonal Hemoglobin alpha Antibody (D-4) yields specific and reliable results in Western blotting of human K-562, HEL 92.1.7, and TF-1 whole cell lysates.
Date published: 2017-01-27
Rated 1 out of 5 by from IHC (P): majority of erythrocytes unstainedFormol-fixed (3,7% in tap water, 24h) normal human spleen specimens; antigen retrievals tested: 1) autoclaving in citrate buffer pH 6.0; 2) protease XIV; no pretreatment With all retrieval techniques the majority of erythrocytes remains unstained.
Date published: 2017-01-18
Rated 5 out of 5 by from Satisfactory Western Blot data of HemoglobinSatisfactory Western Blot data of Hemoglobin expression in human plasma. -SCBT QC
Date published: 2015-02-10
Rated 5 out of 5 by from Nice Western Blot data of Hemoglobin expressionNice Western Blot data of Hemoglobin expression in K-562, HEL 92. -SCBT QC
Date published: 2013-11-25
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Hemoglobin α 抗体 (D-4) is rated 4.3 out of 5 by 8.
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