HEB Antibody (A-9): sc-515484

HEB Antibody (A-9) is a mouse monoclonal IgG2b antibody that detects HEB in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). HEB, a member of the basic helix-loop-helix (bHLH) protein family, plays a crucial role in the regulation of gene expression during muscle cell differentiation. HEB (A-9) antibody targets proteins primarily located in the nucleus, where HEB functions as a transcription factor that forms heterodimers with other bHLH proteins, such as Myo D, to bind to E box elements in the regulatory regions of muscle-specific genes. This interaction is vital for the activation of genes necessary for muscle development, highlighting HEB′s importance in myogenesis and muscle regeneration. The ability of HEB to form both homo- and hetero-oligomers allows for precise regulation of gene expression, ensuring that muscle cells differentiate correctly in response to developmental cues. Anti-HEB antibody (A-9) is an essential tool for researchers studying muscle biology and the molecular mechanisms underlying muscle differentiation and development.

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HEB Antibody (A-9) References:

1. Induction of a diverse T cell receptor gamma/delta repertoire by the helix-loop-helix proteins E2A and HEB in nonlymphoid cells.  |  Ghosh, JK., et al. 2001. J Exp Med. 193: 769-76. PMID: 11257142
2. Studies of the hemato-encephalic barrier. VI. The effect of diaphylline on the penicillin-permeability of the HEB with a note on the treatment of neurosyphilis.  |  KELENTEI, B. and BOCZKO, M. 1956. Confin Neurol. 16: 15-24. PMID: 13343820
3. Phase locking of a 1.5 Terahertz quantum cascade laser and use as a local oscillator in a heterodyne HEB receiver.  |  Rabanus, D., et al. 2009. Opt Express. 17: 1159-68. PMID: 19188942
4. HEB in the spotlight: Transcriptional regulation of T-cell specification, commitment, and developmental plasticity.  |  Braunstein, M. and Anderson, MK. 2012. Clin Dev Immunol. 2012: 678705. PMID: 22577461
5. HEB silencing induces anti-proliferative effects on U87MG cells cultured as neurospheres and monolayers.  |  Godoy, PR., et al. 2016. Mol Med Rep. 14: 5253-5260. PMID: 27779678
6. HEB is required for the specification of fetal IL-17-producing γδ T cells.  |  In, TSH., et al. 2017. Nat Commun. 8: 2004. PMID: 29222418
7. TCF-1 and HEB cooperate to establish the epigenetic and transcription profiles of CD4+CD8+ thymocytes.  |  Emmanuel, AO., et al. 2018. Nat Immunol. 19: 1366-1378. PMID: 30420627
8. Monoallelic Heb/Tcf12 Deletion Reduces the Requirement for NOTCH1 Hyperactivation in T-Cell Acute Lymphoblastic Leukemia.  |  Veiga, DFT., et al. 2022. Front Immunol. 13: 867443. PMID: 35401501
9. Stabilization of β-Catenin Directs HEB to Limit Thymic Selection.  |  Tousinas, G., et al. 2024. J Immunol. 213: 641-650. PMID: 38958395
10. Functional replacement of the mouse E2A gene with a human HEB cDNA.  |  Zhuang, Y., et al. 1998. Mol Cell Biol. 18: 3340-9. PMID: 9584174

The preferred HEB antibody is HEB Antibody (D-3): sc-28364.