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CD44 抗体 (P2A1): sc-53298

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说明书
  • CD44抗体(P2A1)是小鼠单克隆IgG2a κ, 在8篇文献中引用,规格为200 µg/ml
  • 针对眼黑色素瘤细胞系(V+B2)
  • 推荐用于 human 来源的HCAM WB, IP, IF, IHC(P) 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 CD44 (DF1485): sc-7297,可查看有标记的 CD44 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是CD44 Antibody (P2A1) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与CD44 Antibody (P2A1) 打包提供(请参阅下面的订购信息)。
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関連項目

细胞黏附分子(CAMs)是一组密切相关的细胞表面糖蛋白,参与细胞与细胞之间的相互作用,在胚胎发生和发育中发挥重要作用。HCAM又称CD44、LHR、MDU2、MDU3、MIC4、Pgp1、HCELL、MUTCH-I或ECMR-III,是一种由742个氨基酸组成的单通道I型膜蛋白,参与造血、淋巴细胞活化和肿瘤转移。HCAM作为透明质酸(HA)的受体,与骨桥蛋白(OPN)等配体相互作用,介导细胞间和细胞-基质间的相互作用,从而在细胞黏附和细胞迁移中发挥重要作用。HCAM包含一个Link结构域,由于选择性剪接事件,HCAM被表达为多种亚型,其中一些被命名为CD44R, CDw44, CD44S, CD44H(造血)和CD44E(上皮)。虽然大多数HCAM剪接变异体在全身组织中都有表达,但有一种特殊的异构体,即CD44H,在癌组织中高水平表达,这表明CD44H剪接变异体在肿瘤进展中发挥重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD44 抗体 (P2A1) 参考文献:

  1. 新型肼羰基氨基甲基化聚苯乙烯(HCAM)树脂合成 P1-醛蛋白酶候选抑制剂的方法。  |  Siev, DV. and Semple, JE. 2000. Org Lett. 2: 19-22. PMID: 10814235
  2. 正常唾液腺组织和唾液腺恶性肿瘤中的粘附分子NCAM、HCAM、PECAM-1和ICAM-1。  |  Perschbacher, K., et al. 2004. J Oral Pathol Med. 33: 230-6. PMID: 15061711
  3. 免疫组化法检测腮腺 Warthin 肿瘤中细胞粘附分子 E-cadherin, desmoglein-2, β4-integrin, ICAM-1 和 HCAM(CD44s)的表达。  |  Andreadis, D., et al. 2005. Oral Oncol. 41: 799-805. PMID: 16043382
  4. 造血祖细胞表达H-CAM(CD44)归巢相关粘附分子。  |  Lewinsohn, DM., et al. 1990. Blood. 75: 589-95. PMID: 1688719
  5. 荧光生物传感器h钙调素(hCaM)L39C-单溴联苯(mBBr)/V91C-mBBr的开发,这是一种用于发现新的钙调素抑制剂和检测钙的新工具。  |  Gonzalez-Andrade, M., et al. 2011. J Med Chem. 54: 3875-84. PMID: 21495717
  6. Pgp-1的分子克隆和表达。人类H-CAM(Hermes)淋巴细胞归巢受体的鼠类同源物。  |  Zhou, DF., et al. 1989. J Immunol. 143: 3390-5. PMID: 2681416
  7. 开发新型hCaM-Alexa Fluor®生物传感器,用于检测多种配体。  |  Velázquez-López, I., et al. 2017. Anal Biochem. 516: 13-22. PMID: 27744023
  8. 肿瘤性人类组织肥大细胞表达粘附分子CD44/HCAM。  |  Horny, HP., et al. 1996. Virchows Arch. 429: 91-4. PMID: 8917709
  9. 小淋巴细胞和套细胞淋巴瘤中CD44(HCAM)的表达。  |  Aguilera, NS., et al. 1998. Hum Pathol. 29: 1134-9. PMID: 9781654
  10. CD44(H-CAM)在循环和骨髓毛细胞上的表达不同。  |  Rutella, S., et al. 1997. Eur J Histochem. 41 Suppl 2: 49-50. PMID: 9859779

订购信息

产品名称产品编号规格价格数量收藏夹

CD44 抗体 (P2A1)

sc-53298
200 µg/ml
$322.00

CD44 (P2A1): m-IgG Fc BP-HRP 套装

sc-536929
200 µg Ab; 10 µg BP
$361.00

CD44 (P2A1): m-IgGκ BP-HRP 套装

sc-534104
200 µg Ab; 40 µg BP
$361.00

CD44 (P2A1): m-IgG2a BP-HRP 套装

sc-547902
200 µg Ab; 10 µg BP
$361.00

CD44 抗体 (P2A1) FITC

sc-53298 FITC
200 µg/ml
$336.00

CD44 抗体 (P2A1) PE

sc-53298 PE
200 µg/ml
$349.00

anti-CD44 (HCAM-P2A1:sc53298) from Santa Cruz biotechnology, is a monoclonal antibody against CD44 raised against wild-type CD44 or a total antibody that detects other CD44 variants?

Asked by: Trupti Trivedi
Thank you for your question. sc-53298 HCAM (P2A1) has not been epitope mapped. Here is a link for more information on the protein : https://www.uniprot.org/uniprotkb/P16070/entry#sequences
Answered by: BlakeJ
Date published: 2024-08-01

Can HCAM (P2A1): sc-53298 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, HCAM (P2A1): sc-53298 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by jerojero from Excellent!This antibody works great in western blot analysis of HCAM expression in HUV-EC-C, human PBL, HeLa and WI 38 whole cell lysates.
Date published: 2017-01-19
Rated 4 out of 5 by SS from Robust bands detected in western blot analysisRobust bands detected in western blot analysis of HCAM expression in HeLa, U-937, human PBL, HUV-EC-C and CCRF-CEM whole cell lysates. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by TS from Exceptional immunoperoxidase membrane andExceptional immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by FL from Published immunohistochemical analysis ofPublished immunohistochemical analysis of HCAM in human brain, leptomeninges, spinal cord and eye sections. -SCBT Publication Review
Date published: 2015-01-24
Rated 5 out of 5 by JM from Strong bands representing HCAM present inStrong bands representing HCAM present in western blot when using HUV-EC-C, human PBL, HeLa and WI 38 whole cell lysates. -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by SF from Produced superb indirect FCM data for humanProduced superb indirect FCM data for human peripheral blood leukocytes stained with HCAM . -SCBT QC
Date published: 2013-06-25
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CD44 抗体 (P2A1) is rated 4.8 out of 5 by 6.
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