Date published: 2025-9-6

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H-Ras 抗体 (F235): sc-29

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说明书
  • H-Ras 抗体 F235 是小鼠单克隆 IgG1, 在180篇文献中引用,提供 50 µg/0.5 ml
  • 针对重组蛋白
  • 推荐用于 mouse, rat 和 human 来源的H-Ras p21 WB, IP, IF 和 IHC(P)检测
  • 看 H-Ras (259): sc-35,可查看有标记的 H-Ras 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取H-Ras (F235): sc-29免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 H-Ras 抗体 (F235) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与H-Ras 抗体 (F235) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

H-Ras抗体(F235)是一种针对重组蛋白的小鼠单克隆IgG1抗体,专门用于通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP)检测小鼠、大鼠和人类来源的H-Ras p21。H-Ras蛋白,又称Harvey鼠肉瘤病毒癌基因同源物,是Ras基因家族的一员,该家族还包括K-Ras和N-Ras。这些小GTP酶蛋白通过在非活性的GDP结合态和活性的GTP结合态之间循环,在调节细胞生长、分化以及信号转导方面起着关键作用。H-Ras的结构至关重要,它由一个负责GTP/GDP结合和固有GTP酶活性的保守G域以及一个可进行翻译后修饰以定位在细胞膜上的高度可变区域组成。G域的突变会削弱GTP的水解作用,导致H-Ras的持续激活,进而引发不受控制的细胞增殖和肿瘤发生。理解H-Ras结构的重要性在于其在致癌转化中的作用,因为异常激活会扰乱控制细胞增殖和凋亡的过程,使H-Ras成为癌症研究的重要焦点。H-Ras (F235) 单克隆抗体以非结合形式提供,是研究人员研究Ras介导的信号通路和各种癌症的分子机制的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

H-Ras 抗体 (F235) 参考文献:

  1. Ras 的阴暗面:细胞凋亡的调控。  |  Cox, AD. and Der, CJ. 2003. Oncogene. 22: 8999-9006. PMID: 14663478
  2. 前列腺癌进展过程中的 Ras 信号传导。  |  Weber, MJ. and Gioeli, D. 2004. J Cell Biochem. 91: 13-25. PMID: 14689577
  3. 人类 RAS 超家族蛋白和相关 GTP 酶。  |  Colicelli, J. 2004. Sci STKE. 2004: RE13. PMID: 15367757
  4. 肿瘤进展和转移中的致癌 Ras。  |  Giehl, K. 2005. Biol Chem. 386: 193-205. PMID: 15843165
  5. Ras 质膜信号平台。  |  Hancock, JF. and Parton, RG. 2005. Biochem J. 389: 1-11. PMID: 15954863
  6. 高尔基体上的 Ras 信号传导。  |  Quatela, SE. and Philips, MR. 2006. Curr Opin Cell Biol. 18: 162-7. PMID: 16488589
  7. 分区化的 Ras/MAPK 信号传导。  |  Mor, A. and Philips, MR. 2006. Annu Rev Immunol. 24: 771-800. PMID: 16551266
  8. Ras, PI(3)K 和 mTOR 信号控制着肿瘤细胞的生长。  |  Shaw, RJ. and Cantley, LC. 2006. Nature. 441: 424-30. PMID: 16724053
  9. 人类 DNA 中与哺乳动物肉瘤逆转录病毒转化基因相关的三个不同基因。  |  Wong-Staal, F., et al. 1981. Science. 213: 226-8. PMID: 6264598

订购信息

产品名称产品编号规格价格数量收藏夹

H-Ras 抗体 (F235)

sc-29
50 µg/0.5 ml
$316.00

H-Ras (F235): m-IgG Fc BP-HRP 套装

sc-538892
50 µg Ab; 10 µg BP
$354.00

H-Ras (F235): m-IgG1 BP-HRP 套装

sc-541127
50 µg Ab; 20 µg BP
$354.00

What secondary should I use for my Western blot?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-11

I work primarily with porcine samples. Do you know if sc-29 will cross-react?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 6
Date published: 2017-01-11
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Rated 5 out of 5 by from Clear and concise bands probedRequested a sample and used it to probe 293T lysates with conditional expression of Flag-H-RasV12. Clear bands and no spare bands with similar molecular weight.
Date published: 2023-01-31
Rated 5 out of 5 by from Good for IPJust performed IP using this antibody. Great separation and clean background.
Date published: 2019-05-14
Rated 4 out of 5 by from Great featuresI have not purchased this product. leave comments after purchase
Date published: 2017-12-05
Rated 1 out of 5 by from No SignalI tested this antibody by Western blotting HEK298 extracts. I loaded 30 ug of protein and used the antibody at 1:100 dilution. I also used the m-IgGk BP-HRP secondary from SCBT and upon developing the blot, there was no visible band.
Date published: 2017-08-10
Rated 5 out of 5 by from Superb !!Outstanding data for both western blot and IHC staining
Date published: 2017-01-29
Rated 5 out of 5 by from Great for IHCThe H-Ras Antibody (F235): sc-29 works very well for immunohistochemistry with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited over 60 times for further verification.
Date published: 2017-01-16
Rated 5 out of 5 by from Acceptable resultsAcceptable results in the immunohistochemical staining of formalin fixed paraffin embedded tissues of human ccolorectal (FFPE). The antibody was also used in IP and mouse fibroblasts.
Date published: 2017-01-16
Rated 5 out of 5 by from Acceptable results by IHC staining of formalinAcceptable results by IHC staining of formalin fixed, paraffin embedded (FFPE) human ccolorectal tissue.
Date published: 2015-11-11
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H-Ras 抗体 (F235) is rated 4.6 out of 5 by 14.
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