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GST 抗体 (PIP): sc-53585

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说明书
  • GST抗体(PIP)是一种亚美尼亚仓鼠单克隆 IgG1,在200 µg/ml处提供
  • 与GST反应,融合蛋白
  • 推荐用于 GST fusion proteins and glutathione-S-transferase (GST) of Schistosoma japonicum origin WB, IP 和 IF检测
  • 看 GST (B-14): sc-138,可查看有标记的 GST 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成GST 抗体 (PIP)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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    関連項目

    在大肠杆菌中表达真核基因编码区的质粒载体是常用的;这种表达载体通常编码含有部分原核和部分真核特异蛋白的杂合融合蛋白。例如,Smith和Johnson开发的pGEX.3X表达载体允许合成谷胱甘肽-S-转移酶(GST)和插入的cDNA序列编码的蛋白质之间的融合蛋白。来自这些GST融合蛋白的抗体可用于检查斑块和蛋白质印迹中的蛋白质表达,以及用于大肠杆菌中表达的蛋白质的免疫亲和纯化。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    GST 抗体 (PIP) 参考文献:

    1. 谷胱甘肽 S-转移酶(GST)基因家族分析。  |  Nebert, DW. and Vasiliou, V. 2004. Hum Genomics. 1: 460-4. PMID: 15607001
    2. 谷胱甘肽S-转移酶(GST)P1、GSTM1、运动、臭氧和学龄儿童哮喘发病率。  |  Islam, T., et al. 2009. Thorax. 64: 197-202. PMID: 18988661
    3. 谷胱甘肽S-转移酶(GST)融合蛋白的蛋白质-蛋白质相互作用鉴定。  |  Einarson, MB., et al. 2007. CSH Protoc. 2007: pdb.top11. PMID: 21357153
    4. 人正常唾液腺和肿瘤中胎盘谷胱甘肽S-转移酶(GST-pi)的表达。  |  Zieper, M., et al. 1994. Int J Oncol. 5: 961-6. PMID: 21559667
    5. 人脑中的谷胱甘肽S-转移酶。  |  Carder, PJ., et al. 1990. Neuropathol Appl Neurobiol. 16: 293-303. PMID: 2234311
    6. 研究谷胱甘肽S转移酶(GST)多态性在阿联酋人口高血压发展中的作用。  |  Hussain, K., et al. 2012. Iran Red Crescent Med J. 14: 479-82. PMID: 23105984
    7. 谷胱甘肽S-转移酶M1(GSTM1)缺失的功能补偿由另一个GST超家族成员GSTM2完成。  |  Bhattacharjee, P., et al. 2013. Sci Rep. 3: 2704. PMID: 24048194
    8. 4-溴-1,8-萘酰亚胺衍生物作为荧光底物,用于谷胱甘肽S-转移酶(GST)活性的活细胞成像。  |  Fujikawa, Y., et al. 2019. Talanta. 204: 633-640. PMID: 31357346
    9. 人类肌肉谷胱甘肽S-转移酶(GST-4)与人类肝脏GST-1具有高度同源性。  |  Board, PG., et al. 1988. Biochim Biophys Acta. 953: 214-7. PMID: 3281712
    10. 在嗜热四膜虫对 1-氯-2,4-二硝基苯处理的反应性 GST 基因中鉴定谷胱甘肽-S-转移酶 m19 和 m34。  |  Kapkaç, HA. and Arslanyolu, M. 2021. Eur J Protistol. 81: 125838. PMID: 34481325

    订购信息

    产品名称产品编号规格价格数量收藏夹

    GST 抗体 (PIP)

    sc-53585
    200 µg/ml
    $322.00

    What is the recommended starting dilution for Western blot for GST (PIP): sc-53585 monoclonal antibody?

    Asked by: Dr Ninau Qelp
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-53585.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by HF from Clear band observed in Western blot analysisClear band observed in Western blot analysis of recombinant GST under reducing conditions. -SCBT QC
    Date published: 2013-11-26
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    GST 抗体 (PIP) is rated 5.0 out of 5 by 1.
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