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GST Antibody (A-6) - Western Blotting - Image 148973
GST 抗体 (A-6): sc-374171. 日本鼠重组 GST 融合蛋白的 Western 印迹分析.
GST Antibody (A-6) - Western Blotting - Image 148973
GST 抗体 (A-6): sc-374171. 日本鼠重组 GST 融合蛋白的 Western 印迹分析.
GST 抗体 (A-6): sc-374171. 日本鼠重组 GST 融合蛋白的 Western 印迹分析.

GST 抗体 (A-6): sc-374171

5.0(6)
寫評論提問
说明书
  • GST抗体(A-6)是小鼠单克隆IgG1 κ, 在37篇文献中引用，规格为200 µg/ml
  • 针对S. japonicum起源的GST的序列
  • GST 抗体 (A-6) 推荐用于WB, IP, IF 和 ELISA，检测Schistosoma japonicum 来源的 GST fusion proteins and glutathione-S-transferase (GST)
  • 抗GST抗体（A-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP和 m-IgG1 BP-HRP是GST Antibody (A-6) for WB applications. 的首选辅助检测试剂，这些试剂现与GST Antibody (A-6) 打包提供（请参阅下面的订购信息）。
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研究信息

関連項目

GST抗体（A-6）是一种IgG1κ小鼠单克隆GST抗体，可通过WB、IP、IF和ELISA检测日本血吸虫来源的GST融合蛋白和谷胱甘肽S-转移酶（GST）。GST抗体（A-6）既可作为非偶联抗GST抗体形式，也可作为多种偶联形式的抗GST抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。大肠杆菌中真核基因编码区表达的质粒载体是常用的；这种表达载体通常编码含有部分原核和部分真核特异蛋白的杂合融合蛋白。例如，Smith和Johnson开发的pGEX.3X表达载体允许合成谷胱甘肽-S-转移酶（GST）和插入的cDNA序列编码的蛋白质之间的融合蛋白。源自这些GST融合蛋白的抗体可用于检查斑块和蛋白质印迹中的蛋白质表达，以及用于大肠杆菌中表达的蛋白质的免疫亲和纯化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GST 抗体 (A-6) 参考文献:

  1. 谷胱甘肽S-转移酶（GST）P1基因座的多态性是乳腺癌风险调节因子。  |  Maugard, CM., et al. 2001. Int J Cancer. 91: 334-9. PMID: 11169956
  2. 使用GST融合蛋白对调节蛋白结构域进行结构分析。  |  Zhan, Y., et al. 2001. Gene. 281: 1-9. PMID: 11750122
  3. 转基因烟草植物中过表达的棉花谷胱甘肽S-转移酶（GST）对甲基紫精的抗性增强。  |  Yu, T., et al. 2003. J Plant Physiol. 160: 1305-11. PMID: 14658382
  4. 谷胱甘肽S-转移酶（GST）M1基因型缺失和GSTM1和GSTT1基因型缺失的组合是酒精性轻度肝功能障碍的危险因素。  |  Oniki, K., et al. 2007. Clin Pharmacol Ther. 81: 634-5. PMID: 17438538
  5. 切除的肺肿瘤中谷胱甘肽S-转移酶（GST）亚型的表达--与组织学类型和增殖指数的关系。  |  Hatton, T., et al. 1993. Int J Oncol. 3: 881-6. PMID: 21573447
  6. 肿瘤组织中胎盘谷胱甘肽S-转移酶（GST-pi）的升高以及癌症患者血清中的水平。  |  Tsuchida, S., et al. 1989. Cancer Res. 49: 5225-9. PMID: 2504486
  7. 玉米谷胱甘肽S-转移酶（GST I和GST II）的两个编码基因的分子分析和图谱。  |  Rossini, L., et al. 1995. Mol Gen Genet. 248: 535-9. PMID: 7476852
  8. 人类谷胱甘肽S-转移酶mu（GST mu）缺乏是吸烟者易患膀胱癌和喉癌的标志。  |  Lafuente, A., et al. 1993. Cancer Lett. 68: 49-54. PMID: 8422649
  9. 在DMBA诱导仓鼠口腔囊鳞状细胞癌变过程中，胎盘谷胱甘肽S-转移酶（GST-P）的顺序表达。  |  Chen, YK. and Lin, LM. 1996. J Oral Pathol Med. 25: 388-94. PMID: 8890054
  10. 有证据表明人类θ类谷胱甘肽S转移酶T1-1能催化小鼠肝脏和肺部致癌物二氯甲烷的活化。比较人类谷胱甘肽 S 转移酶 T1-1 与 Alpha, Mu 和 Pi 类谷胱甘肽 S 转移酶的组织分布。  |  Sherratt, PJ., et al. 1997. Biochem J. 326 (Pt 3): 837-46. PMID: 9307035

订购信息

产品名称产品编号规格价格数量收藏夹

GST 抗体 (A-6)

sc-374171
200 µg/ml
$322.00

GST (A-6): m-IgG Fc BP-HRP 套装

sc-527440
200 µg Ab; 10 µg BP
$361.00

GST (A-6): m-IgG1 BP-HRP 套装

sc-532813
200 µg Ab; 20 µg BP
$361.00

GST 抗体 (A-6) AC

sc-374171 AC
500 µg/ml, 25% agarose
$424.00

GST 抗体 (A-6) HRP

sc-374171 HRP
200 µg/ml
$322.00

GST 抗体 (A-6) FITC

sc-374171 FITC
200 µg/ml
$336.00

GST 抗体 (A-6) PE

sc-374171 PE
200 µg/ml
$349.00

GST 抗体 (A-6) Alexa Fluor® 488

sc-374171 AF488
200 µg/ml
$364.00

GST 抗体 (A-6) Alexa Fluor® 546

sc-374171 AF546
200 µg/ml
$364.00

GST 抗体 (A-6) Alexa Fluor® 594

sc-374171 AF594
200 µg/ml
$364.00

GST 抗体 (A-6) Alexa Fluor® 647

sc-374171 AF647
200 µg/ml
$364.00

GST 抗体 (A-6) Alexa Fluor® 680

sc-374171 AF680
200 µg/ml
$364.00

GST 抗体 (A-6) Alexa Fluor® 790

sc-374171 AF790
200 µg/ml
$364.00

Can I dilute this antibody in milk?

Asked by: Chuma2011
Thank you for your question. Yes, you can dilute this antibody in 5% milk for Western blot use.
Answered by: Technical Support
Date published: 2019-01-26

What secondary is best to use with GST (A-6): sc-374171 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with GST (A-6): sc-374171, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-12

At what molecular weight should the band be seen in Western Blot with GST (A-6): sc-374171 monoclonal antibody?

Asked by: jenniferc
Thank you for your question. The molecular weight expected varies depending on the fusion protein you are trying to detect. It should equal to the protein of interest plus the weight of the GST tag.
Answered by: Technical Support 10
Date published: 2023-09-04
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Rated 5 out of 5 by schniggi from very nice signal in western blot!used in western blot to detect GST-tagged proteins in budding yeast total protein extract - worked very well at 1:5000 dilution
Date published: 2018-02-08
Rated 5 out of 5 by Bill28 from Strong WB !!Very nice western blot data;strong signal, low background
Date published: 2017-01-28
Rated 5 out of 5 by Trav11 from Strong WB dataThe GST Antibody (A-6): sc-374171 provides good Western blot data showing clear band with minimal background.
Date published: 2017-01-11
Rated 5 out of 5 by JL from Worked well for WB on human CNE2 and DDPWorked well for WB on human CNE2 and DDP cell lysates. -SCBT Publication Review
Date published: 2015-04-15
Rated 5 out of 5 by ET from Published ChIP data using APublished ChIP data using A-6 on mouse thymocyte extracts. -SCBT Publication Review
Date published: 2015-01-17
Rated 5 out of 5 by QZ from Strong signal observed in Western blot analysisStrong signal observed in Western blot analysis of S. japonicum recombinant GST fusion protein. -SCBT QC
Date published: 2013-12-11
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GST 抗体 (A-6) is rated 5.0 out of 5 by 6.
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