Date published: 2026-10-27
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GST Antibody (1E5) - Western Blotting - Image 15625
GST 抗体 (1E5): sc-53909. 重组 GST 蛋白 (A) 和 GST 标记的 Max 融合蛋白 (B) 的 Western 印迹分析.
GST Antibody (1E5) - Western Blotting - Image 15625
GST 抗体 (1E5): sc-53909. 重组 GST 蛋白 (A) 和 GST 标记的 Max 融合蛋白 (B) 的 Western 印迹分析.
GST 抗体 (1E5): sc-53909. 重组 GST 蛋白 (A) 和 GST 标记的 Max 融合蛋白 (B) 的 Western 印迹分析.

GST 抗体 (1E5): sc-53909

4.5(8)
寫評論提問
说明书
  • GST 抗体 1E5 是小鼠单克隆 IgG2b, 在33篇文献中引用，提供 50 µg/500 µl
  • 针对重组谷胱甘肽S-转移酶
  • 推荐用于 GST WB 和 IP检测
  • 看 GST (B-14): sc-138，可查看有标记的 GST 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是GST Antibody (1E5) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与GST Antibody (1E5) 打包提供（请参阅下面的订购信息）。
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研究信息

関連項目

GST抗体（1E5）是一种IgG2b小鼠单克隆GST抗体，可通过WB和IP检测GST。GST抗体（1E5）可以作为非偶联抗GST抗体形式使用。大肠杆菌中真核基因编码区表达的质粒载体是常用的；这种表达载体通常编码含有部分原核和部分真核特异蛋白的杂合融合蛋白。例如，Smith和Johnson开发的pGEX.3X表达载体允许合成谷胱甘肽-S-转移酶（GST）和插入的cDNA序列编码的蛋白质之间的融合蛋白。源自这些GST融合蛋白的抗体可用于检查斑块和蛋白质印迹中的蛋白质表达，以及用于大肠杆菌中表达的蛋白质的免疫亲和纯化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GST 抗体 (1E5) 参考文献:

  1. 谷胱甘肽S-转移酶（GST）M1和GST T1基因型以及苯暴露对造血的影响。  |  Hsieh, LL., et al. 1999. Arch Toxicol. 73: 80-2. PMID: 10350187
  2. 在GSTM1阳性基因型患者的大肠腺癌中，谷胱甘肽S-转移酶（GST）mu表型的缺失。  |  Barker, HJ., et al. 2002. Cancer Lett. 177: 65-74. PMID: 11809532
  3. 谷胱甘肽S-转移酶（GST）M1、T1和N-乙酰转移酶2（NAT2）的多态性与吸烟引起的尿路上皮癌风险。  |  Tsukino, H., et al. 2004. Eur J Cancer Prev. 13: 509-14. PMID: 15548945
  4. 谷胱甘肽-S-转移酶（GST）M1基因缺失和GSTM1和GSTT1基因缺失的组合是丙戊酸治疗患者血清γ-谷氨酰转移酶升高的危险因素。  |  Fukushima, Y., et al. 2008. Clin Chim Acta. 389: 98-102. PMID: 18155166
  5. 谷胱甘肽S-转移酶（GST）基因变异与良性前列腺增生风险：北印度人群报告。  |  Konwar, R., et al. 2010. Asian Pac J Cancer Prev. 11: 1067-72. PMID: 21133626
  6. 谷胱甘肽S-转移酶PI（GST-PI）mRNA表达和DNA甲基化与非小细胞肺癌的发病机制和预后有关。  |  Grimminger, PP., et al. 2012. Lung Cancer. 78: 87-91. PMID: 22884253
  7. 乳腺癌中谷胱甘肽S-转移酶P1基因（GST-pi）的多态性。  |  Khabaz, MN. 2014. Pol J Pathol. 65: 141-6. PMID: 25119175
  8. 谷胱甘肽-S-转移酶（GST）多态性在大肠癌患者中的诊断潜力。  |  Waś, J., et al. 2018. Adv Clin Exp Med. 27: 1561-1566. PMID: 30106268
  9. 西兰花中的谷胱甘肽S-转移酶（GST）同工酶与哺乳动物的θ类谷胱甘肽S-转移酶具有显著的序列同源性。  |  Lopez, MF., et al. 1994. Biochim Biophys Acta. 1205: 29-38. PMID: 8142481
  10. 谷胱甘肽 S 转移酶 Kappa 类：大鼠线粒体 GST 的克隆特征和人类同源物的鉴定。  |  Pemble, SE., et al. 1996. Biochem J. 319 (Pt 3): 749-54. PMID: 8920976

订购信息

产品名称产品编号规格价格数量收藏夹

GST 抗体 (1E5)

sc-53909
50 µg/500 µl
$322.00

GST (1E5): m-IgG Fc BP-HRP 套装

sc-539012
50 µg Ab; 10 µg BP
$361.00

GST (1E5): m-IgG2b BP-HRP 套装

sc-549725
50 µg Ab; 10 µg BP
$361.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 5 out of 5 by cjMara from Excellent for WBThe (1E5) clone is well established and a popular choice for detection of GST fusion proteins in WB.
Date published: 2017-01-07
Rated 5 out of 5 by cxy4868 from Helpful!I used this GST antibody in my WB assay and recieved excellent result. It can indentify my GST tagged fusion protein specifically. So I think its a good product.
Date published: 2016-12-31
Rated 5 out of 5 by Haozai from GST antibody in WBI have already used this GST antibody in my WB assay and recieved excellent result. It can indentify my GST tagged fusion protein specifically.
Date published: 2016-11-24
Rated 1 out of 5 by Xiaoqing from Does not work in Western BlotDoes not work in Western Blot.
Date published: 2015-03-31
Rated 5 out of 5 by HC from Good results with PCGood results with PC-3, DU-145 and LNCaP cell lines in WB -SCBT Publication Review
Date published: 2015-02-19
Rated 5 out of 5 by LF from Used for WB with PCUsed for WB with PC-3, MDCK and COS-7 cell lines -SCBT Publication Review
Date published: 2014-01-09
Rated 5 out of 5 by FL from Detects recombinant GST and GSTDetects recombinant GST and GST-tagged proteins in WB -SCBT QC
Date published: 2013-12-13
Rated 5 out of 5 by SS from Worked in WB with transfected 293T cellsWorked in WB with transfected 293T cells -SCBT Publication Review
Date published: 2013-05-21
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GST抗体 (1E5) is rated 4.5 out of 5 by 8.
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