Date published: 2025-9-6

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GRK 2 抗体 (C-9): sc-13143

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说明书
  • GRK 2 抗体 C-9 是小鼠单克隆 IgG2a κ,GRK 2抗体, 在58篇文献中引用,规格为200 µg/ml
  • 免疫human物种的GRK 2的氨基酸468-689
  • GRK 2 抗体 (C-9) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 GRK 2
  • 抗GRK 2抗体(C-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GRK 2 (C-9): sc-13143免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是GRK 2 Antibody (C-9) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与GRK 2 Antibody (C-9) 打包提供(请参阅下面的订购信息)。

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関連項目

GRK 2 抗体(C-9)是一种小鼠单克隆 IgG2a kappa 轻链抗体,可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人类来源的 GRK 2 蛋白。GRK 2 抗体(C-9)有非结合型和多种结合型,包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 结合物。GRK 2蛋白在调节G蛋白偶联受体信号传导中起着至关重要的作用,而G蛋白偶联受体信号传导对于许多生理过程(包括视觉、味觉和神经传递)至关重要。GRK 2主要位于细胞质和细胞膜中,在受体受刺激时激活,导致受体活化磷酸化。这种磷酸化对于受体的脱敏至关重要,可防止过度刺激并确保细胞对外部信号做出适当反应。GRK 2对受体活性的调节对于心血管健康尤为重要,因为GRK 2有助于调节心率和收缩力。了解GRK 2的功能和调节对于制定针对各种疾病(包括心力衰竭和高血压)的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GRK 2 抗体 (C-9) 参考文献:

  1. 受体激酶家族:犀牛蛋白激酶的初级结构显示出与β-肾上腺素能受体激酶的相似性。  |  Lorenz, W., et al. 1991. Proc Natl Acad Sci U S A. 88: 8715-9. PMID: 1656454
  2. β-肾上腺素能受体激酶 2 的克隆, 表达和染色体定位。受体激酶家族的新成员。  |  Benovic, JL., et al. 1991. J Biol Chem. 266: 14939-46. PMID: 1869533
  3. 关闭信号:β-肾上腺素能受体功能的脱敏。  |  Hausdorff, WP., et al. 1990. FASEB J. 4: 2881-9. PMID: 2165947
  4. GPCR活化和GRK2组装通过偏倚的细胞内激动剂。  |  Duan, J., et al. 2023. Nature. 620: 676-681. PMID: 37532940
  5. GRK2-YAP信号传导与肺动脉高压的发展有关。  |  Ye, P., et al. 2024. Chin Med J (Engl). 137: 846-858. PMID: 38242702
  6. GRK2抑制类风湿性关节炎中Flt-1+巨噬细胞的浸润及其促血管生成特性。  |  Yang, X., et al. 2024. Acta Pharm Sin B. 14: 241-255. PMID: 38261818
  7. 初级纤毛中的GRK2激酶在Hedgehog级联反应中启动SMOOTHENED-PKA信号传导。  |  Walker, MF., et al. 2024. PLoS Biol. 22: e3002685. PMID: 39138140
  8. G 蛋白偶联受体激酶家族成员 GRK5 的鉴定, 纯化和表征。  |  Premont, RT., et al. 1994. J Biol Chem. 269: 6832-41. PMID: 8120045
  9. 研究 G 蛋白偶联受体激酶的一种方法:体外纯化膜测定揭示了不同受体的特异性和 G beta gamma 亚基的调控。  |  Pei, G., et al. 1994. Proc Natl Acad Sci U S A. 91: 3633-6. PMID: 8170959
  10. β-肾上腺素能受体激酶的功能活性靶向结构域:G β gamma 介导的 II 型腺苷酸环化酶刺激抑制剂。  |  Inglese, J., et al. 1994. Proc Natl Acad Sci U S A. 91: 3637-41. PMID: 8170960
  11. G 蛋白偶联受体激酶的结构和机制。  |  Inglese, J., et al. 1993. J Biol Chem. 268: 23735-8. PMID: 8226899
  12. 一种嵌合的 beta 3/beta 2 肾上腺素能受体揭示了受体亚型特异性调节的结构基础。  |  Liggett, SB., et al. 1993. Proc Natl Acad Sci U S A. 90: 3665-9. PMID: 8386380

订购信息

产品名称产品编号规格价格数量收藏夹

GRK 2 抗体 (C-9)

sc-13143
200 µg/ml
$316.00

GRK 2 (C-9): m-IgG Fc BP-HRP 套装

sc-528269
200 µg Ab; 10 µg BP
$354.00

GRK 2 (C-9): m-IgGκ BP-HRP 套装

sc-520606
200 µg Ab, 40 µg BP
$354.00

GRK 2 抗体 (C-9) AC

sc-13143 AC
500 µg/ml, 25% agarose
$416.00

GRK 2 抗体 (C-9) HRP

sc-13143 HRP
200 µg/ml
$316.00

GRK 2 抗体 (C-9) FITC

sc-13143 FITC
200 µg/ml
$330.00

GRK 2 抗体 (C-9) PE

sc-13143 PE
200 µg/ml
$343.00

GRK 2 抗体 (C-9) Alexa Fluor® 488

sc-13143 AF488
200 µg/ml
$357.00

GRK 2 抗体 (C-9) Alexa Fluor® 546

sc-13143 AF546
200 µg/ml
$357.00

GRK 2 抗体 (C-9) Alexa Fluor® 594

sc-13143 AF594
200 µg/ml
$357.00

GRK 2 抗体 (C-9) Alexa Fluor® 647

sc-13143 AF647
200 µg/ml
$357.00

GRK 2 抗体 (C-9) Alexa Fluor® 680

sc-13143 AF680
200 µg/ml
$357.00

GRK 2 抗体 (C-9) Alexa Fluor® 790

sc-13143 AF790
200 µg/ml
$357.00

What is the purification method for the antibody cat no 13143?

Asked by: Pia Hansson
Thank you for your question. This mouse monoclonal antibody is produced using standard mouse monoclonal production methods. During purification, antibodies from subcloned isolated cells are purified with a protein G column, and tested for specificity throughout the process using ELISA and WB. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-03-17

What is the recommended secondary for this mouse primary antibody?

Asked by: AbPolly
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2023-11-03
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Rated 5 out of 5 by from 抗体不错,老板满意找了很久,帮老板找到这个抗体,效果不错,价格低,品质OK。。以后还会继续购买SCBT的抗体。。希望越做越好
Date published: 2018-06-08
Rated 4 out of 5 by from 功能出色这支抗体整体性能一般。以开始我是做出来了,后来师妹做的时候,不知道怎么回事,没有WORK。表现一般般。
Date published: 2018-01-13
Rated 5 out of 5 by from Great AntibodyI used this antibody to test my GRK2 protein that was produced through the baculovirus expression system in Sf9 cells. This antibody has worked twice for me now. I highly recommend this antibody for anyone working with Rattus norvegicus GRK2 protein.
Date published: 2017-10-17
Rated 5 out of 5 by from signal is very strongWe used this antibody to detect GRK 2, result is very specific band.
Date published: 2017-09-12
Rated 5 out of 5 by from strongI use the antibody to detect GRK 2 expression in HL-60 whole cell lysates, it performs very well.
Date published: 2017-08-15
Rated 5 out of 5 by from Good for WB1:500 diluted with bsa for the wb of rat protein,the band is strong ,but the background is a little black.
Date published: 2017-06-19
Rated 5 out of 5 by from strong bandNice and strong bands in detect human brain tissue with very low background.
Date published: 2017-06-03
Rated 5 out of 5 by from strong signalIF was used on a brain tissue extract of human, dilution 1:400 (1 ml of cell lysate),worked well ,got strong signal.
Date published: 2017-05-28
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GRK 2 抗体 (C-9) is rated 4.7 out of 5 by 14.
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