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GR/NR3C1/Glucocorticoid Receptor Antibody (G-5) - Western Blotting - Image 391357
GR 抗体 (G-5) Alexa Fluor® 488: sc-393232 AF488. A-431 (A), HeLa (C) 和 NIH/3T3 (D) 全细胞裂解液及 A-431 核提取物 (B) 中 GR 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
GR 抗体 (G-5) Alexa Fluor® 488: sc-393232 AF488. A-431 (A), HeLa (C) 和 NIH/3T3 (D) 全细胞裂解液及 A-431 核提取物 (B) 中 GR 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5): sc-393232

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寫評論提問

说明书
  • GR/NR3C1/Glucocorticoid Receptor 抗体 G-5 是小鼠单克隆 IgG2b κ，GR/NR3C1/Glucocorticoid Receptor抗体, 在98篇文献中引用，规格为200 µg/ml
  • 免疫human物种的GR的氨基酸121-420
  • GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GR α and GR β
  • 抗GR/NR3C1/Glucocorticoid Receptor抗体（G-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-393232 X, 200 µg/0.1 ml)
  • 关于如何获取GR/NR3C1/Glucocorticoid Receptor (G-5): sc-393232的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

GR抗体（G-5）是一种IgG2b κ小鼠单克隆GR抗体（也称为糖皮质激素受体抗体或NR3C1抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源的GR蛋白。GR抗体（G-5）既可作为非偶联的抗GR抗体形式提供，也可作为多种偶联形式的抗GR抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。糖皮质激素受体（GR）是一种普遍表达的转录因子，介导糖皮质激素的作用。最丰富的同工型是GRα。GR诱导或抑制基因表达以响应糖皮质激素，介导细胞凋亡、细胞生长和分化等过程。GR抑制的一类重要基因由转录因子AP-1控制。GR也被证明是糖皮质激素诱导基因表达的限制因素。已经发现GR与HSP 90形成复合物，使非配体结合受体转录失活。更重要的是，缺乏信号传导域的突变GR保持组成型活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5)

sc-393232
200 µg/ml
$316.00

GR/NR3C1/Glucocorticoid Receptor (G-5): m-IgG Fc BP-HRP 套装

sc-526231
200 µg Ab; 10 µg BP
$354.00

GR/NR3C1/Glucocorticoid Receptor (G-5): m-IgG2b BP-HRP 套装

sc-549698
200 µg Ab; 10 µg BP
$354.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) X

sc-393232 X
200 µg/0.1 ml
$316.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) AC

sc-393232 AC
500 µg/ml, 25% agarose
$416.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) HRP

sc-393232 HRP
200 µg/ml
$316.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) FITC

sc-393232 FITC
200 µg/ml
$330.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) PE

sc-393232 PE
200 µg/ml
$343.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) Alexa Fluor® 488

sc-393232 AF488
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) Alexa Fluor® 546

sc-393232 AF546
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) Alexa Fluor® 594

sc-393232 AF594
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) Alexa Fluor® 647

sc-393232 AF647
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) Alexa Fluor® 680

sc-393232 AF680
200 µg/ml
$357.00

GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) Alexa Fluor® 790

sc-393232 AF790
200 µg/ml
$357.00

RRID?

Asked by: lyh15
Thank you for your question. RRID:AB_2687823
Answered by: Ling Chen
Date published: 2024-12-25

I have delta GR in which we have only the c-terminus of GR from 506 aa to the 794 aa so which antibody would be suitable for this deleted version of GR?

Asked by: KIRTIRAVAT
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service by phone or email. Please call (800)-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2024-10-17

is this antibody applicable to the IHC/IP/WB plant model system?

Asked by: KIRTIRAVAT
Thank you for your question. GR/NR3C1/Glucocorticoid Receptor Antibody (G-5): sc-393232 is recommended for detection of GR α and GR β of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2024-10-15

Setting: Western Blot 20 mcg protein of tissue lysate (mouse tissue); antibody diluted 1:1000 in 5%BSA. Problem: I have a good band at 95 kDa , but also a clear (unexpected) band around 50kDa. What could this be?

Asked by: Arnot
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

Does GR antibody (G-5): sc-393232 work in ChIP assay for rat muscle?

Asked by: jymrobin
Thank you for your question. This antibody has been published previously for ChIP applications (PMID: 30994266 and PMID: 30698747). This antibody is reactive with mouse, rat and human. We do not have any data specifically in rat muscle.
Answered by: Technical Support
Date published: 2019-07-18

How well does the antibody work with rat brain tissue for western blotting, and what would be the optimum concentration?

Asked by: nico315
Thank you for your question. We have not yet tested this antibody in rat brain tissue. We do have data in mouse brain tissue extract. The primary antibody was used at the starting dilution of 1:200, and loading 50ug tissue extract.
Answered by: Technical Support
Date published: 2019-07-13

Is the GR ANTIBODY (G-5) X sc-393232X the same as the GR ANTIBODY (G-5) sc-393232 but just at a higher concentration?

Asked by: MegCon
Thank you for your question. Yes, sc-393232 X is provided at a 10x higher concentration. It is provided as 200µg in 0.1ml while the standard product is provided as 200µg in 1ml.
Answered by: Tech Service
Date published: 2019-01-14

Hi, please let me know what concentration of the  GR Antibody (G-5): sc-393232 would be optimal for  Flow Cytometry.  Thank you.

Asked by: nacharya18
Thank you for your question. We have not yet tested this mouse monoclonal, GR Antibody (G-5): sc-393232, for Flow Cytometry so this application is not recommended at this time. We do offer this antibody directly conjugated to a variety of fluorochromes, including: phycoerythrin (sc-393232 PE), fluorescein (sc-393232 FITC), Alexa Fluor® 488 (sc-393232 AF488) or Alexa Fluor® 647 (sc-393232 AF647), 200 µg/ml, for IF, IHC(P) and FCM. If you purchase one of the direct conjugates, we can extend our product warranty to cover the application of Flow Cytometry. A link to our recommended protocol for FCM is below. We recommend using approximately 1 µg of fluorochrome-conjugated antibody to 100 µl of the prepared cell suspension (equal to 1 million cells) to each tube. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2017-03-03
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Rated 5 out of 5 by from Works well in mouse brain at 1:100!We bought this antibody last month. We ran a dilution series in mouse brain on free floating 50-micron sections for the following primary antibody dilution amounts: 1:50, 1:100, and 1:200 (+ a no primary control). There was minimal difference between these concentrations and no staining present in the no primary control so we plan to proceed by using it at 1:100.
Date published: 2021-12-14
Rated 4 out of 5 by from Good for WB of mouse hippocampusWe ordered the sample and proved it for WB of mice hippocampal tissue. Gave a nice specific band for the GR as assessed in WT and Emx1-Nr3c1 KO mice.
Date published: 2021-08-03
Rated 4 out of 5 by from Glucocorticoid receptor antibodyGood staining of mice hypothalamus. Sample: mouse cortex fixed with 4% PFA for 16hr at 4℃, followed by sucrose infiltration and OCT embedding. Staining: floatation staining for 48hr at 4℃ (1:300 or 1:500, 300 was better), followed by 2nd antibody staining. FYI, I have not tried shorter staining such as 1hr at RT or overnight at 4℃.
Date published: 2021-07-28
Rated 3 out of 5 by from Glucocorticoid receptor antibodyThis antibody works better in nuclear then in cytoplasmic fraction of liver (dilution 1:250).
Date published: 2021-02-17
Rated 3 out of 5 by from Glucocorticoid receptor antibodyThis antibody works better in nuclear then in cytoplasmic fraction of liver (dilution 1:250).
Date published: 2021-02-17
Rated 5 out of 5 by from Works excelent in westernblotGR was detected in mesenchymal stem cells and used at a dilution of 1;250 1 H at room temp
Date published: 2020-09-18
Rated 5 out of 5 by from PERFECT CHOICEThis antibody works well. Used 1:500 in tbs 0,1%tween to detect GR in different cell line extract (45gs of total proteins; MCF-7 breast cancer cells and KP2 and SUIT2 pancreatic cancer cells), detect only one specific band at 95 kDa. I’ll buy this antibody.
Date published: 2019-09-27
Rated 5 out of 5 by from Works well!It works well for ChIP on both mouse hepatocytes and mouse livers.
Date published: 2019-04-26
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GR/NR3C1/Glucocorticoid Receptor 抗体 (G-5) is rated 4.1 out of 5 by 28.
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